Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells

Akiko Kubota, Rui Kano, Takuya Mizuno, Masaharu Hisasue, Peter F Moore, Toshihiro Watari, Hajime Tsujimoto, Atsuhiko Hasegawa

Research output: Contribution to journalArticle

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Abstract

Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/d/. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.

Original languageEnglish (US)
Pages (from-to)835-837
Number of pages3
JournalJournal of Veterinary Medical Science
Volume64
Issue number9
DOIs
StatePublished - Sep 2002

Fingerprint

Parathyroid Hormone-Related Protein
parathyroid hormone
lymphoma
Lymphoma
Dogs
hypercalcemia
Hypercalcemia
dogs
proteins
cells
Canidae
assays
cell lines
Calcium
calcium
sampling
Cell Line
Messenger RNA

Keywords

  • Hypercalcemia
  • Lymphoma cell
  • mRNA
  • Parathyroid hormone-related protein
  • RT-PCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells. / Kubota, Akiko; Kano, Rui; Mizuno, Takuya; Hisasue, Masaharu; Moore, Peter F; Watari, Toshihiro; Tsujimoto, Hajime; Hasegawa, Atsuhiko.

In: Journal of Veterinary Medical Science, Vol. 64, No. 9, 09.2002, p. 835-837.

Research output: Contribution to journalArticle

Kubota, A, Kano, R, Mizuno, T, Hisasue, M, Moore, PF, Watari, T, Tsujimoto, H & Hasegawa, A 2002, 'Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells', Journal of Veterinary Medical Science, vol. 64, no. 9, pp. 835-837. https://doi.org/10.1292/jvms.64.835
Kubota, Akiko ; Kano, Rui ; Mizuno, Takuya ; Hisasue, Masaharu ; Moore, Peter F ; Watari, Toshihiro ; Tsujimoto, Hajime ; Hasegawa, Atsuhiko. / Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells. In: Journal of Veterinary Medical Science. 2002 ; Vol. 64, No. 9. pp. 835-837.
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