Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells

Akiko Kubota, Rui Kano, Takuya Mizuno, Masaharu Hisasue, Peter F Moore, Toshihiro Watari, Hajime Tsujimoto, Atsuhiko Hasegawa

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/d/. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.

Original languageEnglish (US)
Pages (from-to)835-837
Number of pages3
JournalJournal of Veterinary Medical Science
Volume64
Issue number9
DOIs
StatePublished - Sep 2002

Keywords

  • Hypercalcemia
  • Lymphoma cell
  • mRNA
  • Parathyroid hormone-related protein
  • RT-PCR

ASJC Scopus subject areas

  • veterinary(all)

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    Kubota, A., Kano, R., Mizuno, T., Hisasue, M., Moore, P. F., Watari, T., Tsujimoto, H., & Hasegawa, A. (2002). Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells. Journal of Veterinary Medical Science, 64(9), 835-837. https://doi.org/10.1292/jvms.64.835