Abstract
Background. Burn injury often causes multiple organ failure as well as skin damage. Several studies suggest that TNF-α plays an important role in postinjury immunosuppression by altering lymphoid tissues. We investigated the regulation of TNF-α expression and apoptosis in the spleen and thymus of mice after burn injury. Materials and methods. C57BLKS/J mice were subjected to 18% TBSA full-thickness burn and the spleen and thymus were harvested at various time points (3 h to 29 days). The expression of TNF-α mRNA and protein in tissue extracts was analyzed by RT-PCR and ELISA. Apoptosis was measured by flow cytometry using annexin V staining. Results. Burn injury induced TNF-α mRNA expression in the thymus at Day 1 and it returned to the basal levels at Day 14 and thereafter. Similarly, TNF-α mRNA up-regulation peaked between Day 1 and Day 3 in the spleen. Induction of TNF-α protein peaked at Day 1 in the thymus, whereas, TNF-α protein was unchanged in the spleen after burn injury. There was a twofold increase in apoptotic cells at Day 1 in the thymus, which is consistent with mRNA and protein data. In contrast, burn injury did not change apoptotic events in the spleen. Conclusions. The parallel induction of TNF-α mRNA, TNF-α protein, and apoptosis suggests that TNF-α may contribute to immunosuppression after burn injury by inducing apoptosis in the thymus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-15 |
| Number of pages | 7 |
| Journal | Journal of Surgical Research |
| Volume | 98 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1 2001 |
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Keywords
- Apoptosis
- Burn injury
- Mice
- Spleen
- Thymus
- TNF-α
ASJC Scopus subject areas
- Surgery
Cite this
Parallel self-induction of TNF-α and apoptosis in the thymus of mice after burn injury. / Cho, Kiho; Adamson, Lee K.; Greenhalgh, David G.
In: Journal of Surgical Research, Vol. 98, No. 1, 01.06.2001, p. 9-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Parallel self-induction of TNF-α and apoptosis in the thymus of mice after burn injury
AU - Cho, Kiho
AU - Adamson, Lee K.
AU - Greenhalgh, David G
PY - 2001/6/1
Y1 - 2001/6/1
N2 - Background. Burn injury often causes multiple organ failure as well as skin damage. Several studies suggest that TNF-α plays an important role in postinjury immunosuppression by altering lymphoid tissues. We investigated the regulation of TNF-α expression and apoptosis in the spleen and thymus of mice after burn injury. Materials and methods. C57BLKS/J mice were subjected to 18% TBSA full-thickness burn and the spleen and thymus were harvested at various time points (3 h to 29 days). The expression of TNF-α mRNA and protein in tissue extracts was analyzed by RT-PCR and ELISA. Apoptosis was measured by flow cytometry using annexin V staining. Results. Burn injury induced TNF-α mRNA expression in the thymus at Day 1 and it returned to the basal levels at Day 14 and thereafter. Similarly, TNF-α mRNA up-regulation peaked between Day 1 and Day 3 in the spleen. Induction of TNF-α protein peaked at Day 1 in the thymus, whereas, TNF-α protein was unchanged in the spleen after burn injury. There was a twofold increase in apoptotic cells at Day 1 in the thymus, which is consistent with mRNA and protein data. In contrast, burn injury did not change apoptotic events in the spleen. Conclusions. The parallel induction of TNF-α mRNA, TNF-α protein, and apoptosis suggests that TNF-α may contribute to immunosuppression after burn injury by inducing apoptosis in the thymus.
AB - Background. Burn injury often causes multiple organ failure as well as skin damage. Several studies suggest that TNF-α plays an important role in postinjury immunosuppression by altering lymphoid tissues. We investigated the regulation of TNF-α expression and apoptosis in the spleen and thymus of mice after burn injury. Materials and methods. C57BLKS/J mice were subjected to 18% TBSA full-thickness burn and the spleen and thymus were harvested at various time points (3 h to 29 days). The expression of TNF-α mRNA and protein in tissue extracts was analyzed by RT-PCR and ELISA. Apoptosis was measured by flow cytometry using annexin V staining. Results. Burn injury induced TNF-α mRNA expression in the thymus at Day 1 and it returned to the basal levels at Day 14 and thereafter. Similarly, TNF-α mRNA up-regulation peaked between Day 1 and Day 3 in the spleen. Induction of TNF-α protein peaked at Day 1 in the thymus, whereas, TNF-α protein was unchanged in the spleen after burn injury. There was a twofold increase in apoptotic cells at Day 1 in the thymus, which is consistent with mRNA and protein data. In contrast, burn injury did not change apoptotic events in the spleen. Conclusions. The parallel induction of TNF-α mRNA, TNF-α protein, and apoptosis suggests that TNF-α may contribute to immunosuppression after burn injury by inducing apoptosis in the thymus.
KW - Apoptosis
KW - Burn injury
KW - Mice
KW - Spleen
KW - Thymus
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=0035370442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035370442&partnerID=8YFLogxK
U2 - 10.1006/jsre.2001.6157
DO - 10.1006/jsre.2001.6157
M3 - Article
C2 - 11368531
AN - SCOPUS:0035370442
VL - 98
SP - 9
EP - 15
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 1
ER -