Parallel self-induction of TNF-α and apoptosis in the thymus of mice after burn injury

Kiho Cho, Lee K. Adamson, David G Greenhalgh

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background. Burn injury often causes multiple organ failure as well as skin damage. Several studies suggest that TNF-α plays an important role in postinjury immunosuppression by altering lymphoid tissues. We investigated the regulation of TNF-α expression and apoptosis in the spleen and thymus of mice after burn injury. Materials and methods. C57BLKS/J mice were subjected to 18% TBSA full-thickness burn and the spleen and thymus were harvested at various time points (3 h to 29 days). The expression of TNF-α mRNA and protein in tissue extracts was analyzed by RT-PCR and ELISA. Apoptosis was measured by flow cytometry using annexin V staining. Results. Burn injury induced TNF-α mRNA expression in the thymus at Day 1 and it returned to the basal levels at Day 14 and thereafter. Similarly, TNF-α mRNA up-regulation peaked between Day 1 and Day 3 in the spleen. Induction of TNF-α protein peaked at Day 1 in the thymus, whereas, TNF-α protein was unchanged in the spleen after burn injury. There was a twofold increase in apoptotic cells at Day 1 in the thymus, which is consistent with mRNA and protein data. In contrast, burn injury did not change apoptotic events in the spleen. Conclusions. The parallel induction of TNF-α mRNA, TNF-α protein, and apoptosis suggests that TNF-α may contribute to immunosuppression after burn injury by inducing apoptosis in the thymus.

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalJournal of Surgical Research
Volume98
Issue number1
DOIs
StatePublished - Jun 1 2001

Fingerprint

Thymus Gland
Apoptosis
Spleen
Wounds and Injuries
Messenger RNA
Proteins
Immunosuppression
Tissue Extracts
Multiple Organ Failure
Annexin A5
Lymphoid Tissue
Flow Cytometry
Up-Regulation
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Polymerase Chain Reaction
Skin

Keywords

  • Apoptosis
  • Burn injury
  • Mice
  • Spleen
  • Thymus
  • TNF-α

ASJC Scopus subject areas

  • Surgery

Cite this

Parallel self-induction of TNF-α and apoptosis in the thymus of mice after burn injury. / Cho, Kiho; Adamson, Lee K.; Greenhalgh, David G.

In: Journal of Surgical Research, Vol. 98, No. 1, 01.06.2001, p. 9-15.

Research output: Contribution to journalArticle

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abstract = "Background. Burn injury often causes multiple organ failure as well as skin damage. Several studies suggest that TNF-α plays an important role in postinjury immunosuppression by altering lymphoid tissues. We investigated the regulation of TNF-α expression and apoptosis in the spleen and thymus of mice after burn injury. Materials and methods. C57BLKS/J mice were subjected to 18{\%} TBSA full-thickness burn and the spleen and thymus were harvested at various time points (3 h to 29 days). The expression of TNF-α mRNA and protein in tissue extracts was analyzed by RT-PCR and ELISA. Apoptosis was measured by flow cytometry using annexin V staining. Results. Burn injury induced TNF-α mRNA expression in the thymus at Day 1 and it returned to the basal levels at Day 14 and thereafter. Similarly, TNF-α mRNA up-regulation peaked between Day 1 and Day 3 in the spleen. Induction of TNF-α protein peaked at Day 1 in the thymus, whereas, TNF-α protein was unchanged in the spleen after burn injury. There was a twofold increase in apoptotic cells at Day 1 in the thymus, which is consistent with mRNA and protein data. In contrast, burn injury did not change apoptotic events in the spleen. Conclusions. The parallel induction of TNF-α mRNA, TNF-α protein, and apoptosis suggests that TNF-α may contribute to immunosuppression after burn injury by inducing apoptosis in the thymus.",
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N2 - Background. Burn injury often causes multiple organ failure as well as skin damage. Several studies suggest that TNF-α plays an important role in postinjury immunosuppression by altering lymphoid tissues. We investigated the regulation of TNF-α expression and apoptosis in the spleen and thymus of mice after burn injury. Materials and methods. C57BLKS/J mice were subjected to 18% TBSA full-thickness burn and the spleen and thymus were harvested at various time points (3 h to 29 days). The expression of TNF-α mRNA and protein in tissue extracts was analyzed by RT-PCR and ELISA. Apoptosis was measured by flow cytometry using annexin V staining. Results. Burn injury induced TNF-α mRNA expression in the thymus at Day 1 and it returned to the basal levels at Day 14 and thereafter. Similarly, TNF-α mRNA up-regulation peaked between Day 1 and Day 3 in the spleen. Induction of TNF-α protein peaked at Day 1 in the thymus, whereas, TNF-α protein was unchanged in the spleen after burn injury. There was a twofold increase in apoptotic cells at Day 1 in the thymus, which is consistent with mRNA and protein data. In contrast, burn injury did not change apoptotic events in the spleen. Conclusions. The parallel induction of TNF-α mRNA, TNF-α protein, and apoptosis suggests that TNF-α may contribute to immunosuppression after burn injury by inducing apoptosis in the thymus.

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