Parallel analysis of individual biological cells using multifocal laser tweezers Raman spectroscopy

Rui Liu, Douglas S. Taylor, Dennis L Matthews, James W Chan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying singlecell dynamics of cell populations and for the development of highthroughput Raman based cytometers.

Original languageEnglish (US)
Pages (from-to)1308-1310
Number of pages3
JournalApplied Spectroscopy
Volume64
Issue number11
DOIs
StatePublished - Nov 2010

Fingerprint

laser spectroscopy
Raman spectroscopy
Lasers
cells
time sharing
crosstalk
beads
lasers
slits
charge coupled devices
Polystyrenes
polystyrene
acquisition
Crosstalk
semiconductor lasers
trapping
Charge coupled devices
traps
spectrometers
Semiconductor lasers

Keywords

  • Multifocal
  • Optical tweezers
  • Raman spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Parallel analysis of individual biological cells using multifocal laser tweezers Raman spectroscopy. / Liu, Rui; Taylor, Douglas S.; Matthews, Dennis L; Chan, James W.

In: Applied Spectroscopy, Vol. 64, No. 11, 11.2010, p. 1308-1310.

Research output: Contribution to journalArticle

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