Parallel analysis of individual biological cells using multifocal laser tweezers Raman spectroscopy

Rui Liu, Douglas S. Taylor, Dennis L Matthews, James W Chan

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying singlecell dynamics of cell populations and for the development of highthroughput Raman based cytometers.

Original languageEnglish (US)
Pages (from-to)1308-1310
Number of pages3
JournalApplied Spectroscopy
Volume64
Issue number11
DOIs
StatePublished - Nov 2010

Keywords

  • Multifocal
  • Optical tweezers
  • Raman spectroscopy

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

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