Abstract
Endothelial nitric oxide synthase (eNOS) or NOS-III in the endothelium catalyzes production of nitric oxide (NO). Nitric oxide diffuses freely into vascular smooth muscle, where it activates soluble guanylate cyclase (sGC) to produce guanosine 3′,5′-cyclic monophosphate (cGMP) and causes vasorelaxation. The NO/cGMP pathway is an important signaling pathway in the control of perinatal pulmonary circulation. An exact colocalization of NOS-III in the pulmonary endothelium and sGC in the vascular smooth muscle was demonstrated using a double immunolabeling technique. The sGC immunoreactivity was higher in resistant pulmonary vessels and veins than in conduit arteries, whereas NOS-III immunoreactivity was higher in conduit arteries than in veins. These results demonstrated anatomically in situ a paracrine role of NOS-III and sGC in the regulation of fetal pulmonary circulation and suggested a heterogeneous distribution of NOS-III and sGC within fetal ovine pulmonary vasculature. Our results provided an anatomic basis that supported previous functional studies on perinatal control of pulmonary circulation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| Journal | Histochemistry and Cell Biology |
| Volume | 119 |
| Issue number | 2 |
| State | Published - Feb 1 2003 |
| Externally published | Yes |
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Keywords
- Double immunolabeling
- Fetus
- Lung
- NOS
- sGC
ASJC Scopus subject areas
- Cell Biology
- Instrumentation
Cite this
Paracrine role of soluble guanylate cyclase and type III nitric oxide synthase in ovine fetal pulmonary circulation : A double labeling immunohistochemical study. / Tzao, Ching; Nickerson, Peter A.; Russell, James A.; Noble, Bernice K.; Steinhorn, Robin H.
In: Histochemistry and Cell Biology, Vol. 119, No. 2, 01.02.2003, p. 125-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Paracrine role of soluble guanylate cyclase and type III nitric oxide synthase in ovine fetal pulmonary circulation
T2 - A double labeling immunohistochemical study
AU - Tzao, Ching
AU - Nickerson, Peter A.
AU - Russell, James A.
AU - Noble, Bernice K.
AU - Steinhorn, Robin H
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Endothelial nitric oxide synthase (eNOS) or NOS-III in the endothelium catalyzes production of nitric oxide (NO). Nitric oxide diffuses freely into vascular smooth muscle, where it activates soluble guanylate cyclase (sGC) to produce guanosine 3′,5′-cyclic monophosphate (cGMP) and causes vasorelaxation. The NO/cGMP pathway is an important signaling pathway in the control of perinatal pulmonary circulation. An exact colocalization of NOS-III in the pulmonary endothelium and sGC in the vascular smooth muscle was demonstrated using a double immunolabeling technique. The sGC immunoreactivity was higher in resistant pulmonary vessels and veins than in conduit arteries, whereas NOS-III immunoreactivity was higher in conduit arteries than in veins. These results demonstrated anatomically in situ a paracrine role of NOS-III and sGC in the regulation of fetal pulmonary circulation and suggested a heterogeneous distribution of NOS-III and sGC within fetal ovine pulmonary vasculature. Our results provided an anatomic basis that supported previous functional studies on perinatal control of pulmonary circulation.
AB - Endothelial nitric oxide synthase (eNOS) or NOS-III in the endothelium catalyzes production of nitric oxide (NO). Nitric oxide diffuses freely into vascular smooth muscle, where it activates soluble guanylate cyclase (sGC) to produce guanosine 3′,5′-cyclic monophosphate (cGMP) and causes vasorelaxation. The NO/cGMP pathway is an important signaling pathway in the control of perinatal pulmonary circulation. An exact colocalization of NOS-III in the pulmonary endothelium and sGC in the vascular smooth muscle was demonstrated using a double immunolabeling technique. The sGC immunoreactivity was higher in resistant pulmonary vessels and veins than in conduit arteries, whereas NOS-III immunoreactivity was higher in conduit arteries than in veins. These results demonstrated anatomically in situ a paracrine role of NOS-III and sGC in the regulation of fetal pulmonary circulation and suggested a heterogeneous distribution of NOS-III and sGC within fetal ovine pulmonary vasculature. Our results provided an anatomic basis that supported previous functional studies on perinatal control of pulmonary circulation.
KW - Double immunolabeling
KW - Fetus
KW - Lung
KW - NOS
KW - sGC
UR - http://www.scopus.com/inward/record.url?scp=0037297349&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037297349&partnerID=8YFLogxK
M3 - Article
C2 - 12610731
AN - SCOPUS:0037297349
VL - 119
SP - 125
EP - 130
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 2
ER -