Paracrine role of soluble guanylate cyclase and type III nitric oxide synthase in ovine fetal pulmonary circulation: A double labeling immunohistochemical study

Ching Tzao, Peter A. Nickerson, James A. Russell, Bernice K. Noble, Robin H Steinhorn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Endothelial nitric oxide synthase (eNOS) or NOS-III in the endothelium catalyzes production of nitric oxide (NO). Nitric oxide diffuses freely into vascular smooth muscle, where it activates soluble guanylate cyclase (sGC) to produce guanosine 3′,5′-cyclic monophosphate (cGMP) and causes vasorelaxation. The NO/cGMP pathway is an important signaling pathway in the control of perinatal pulmonary circulation. An exact colocalization of NOS-III in the pulmonary endothelium and sGC in the vascular smooth muscle was demonstrated using a double immunolabeling technique. The sGC immunoreactivity was higher in resistant pulmonary vessels and veins than in conduit arteries, whereas NOS-III immunoreactivity was higher in conduit arteries than in veins. These results demonstrated anatomically in situ a paracrine role of NOS-III and sGC in the regulation of fetal pulmonary circulation and suggested a heterogeneous distribution of NOS-III and sGC within fetal ovine pulmonary vasculature. Our results provided an anatomic basis that supported previous functional studies on perinatal control of pulmonary circulation.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalHistochemistry and Cell Biology
Volume119
Issue number2
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

pulmonary circulation
Pulmonary Circulation
Nitric Oxide Synthase Type III
Nitric oxide
nitric oxide
Labeling
marking
Sheep
endothelium
smooth muscle
arteries
veins
Nitric Oxide
Muscle
Vascular Smooth Muscle
Endothelium
guanosines
Arteries
Lung
Pulmonary Veins

Keywords

  • Double immunolabeling
  • Fetus
  • Lung
  • NOS
  • sGC

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Paracrine role of soluble guanylate cyclase and type III nitric oxide synthase in ovine fetal pulmonary circulation : A double labeling immunohistochemical study. / Tzao, Ching; Nickerson, Peter A.; Russell, James A.; Noble, Bernice K.; Steinhorn, Robin H.

In: Histochemistry and Cell Biology, Vol. 119, No. 2, 01.02.2003, p. 125-130.

Research output: Contribution to journalArticle

Tzao, Ching ; Nickerson, Peter A. ; Russell, James A. ; Noble, Bernice K. ; Steinhorn, Robin H. / Paracrine role of soluble guanylate cyclase and type III nitric oxide synthase in ovine fetal pulmonary circulation : A double labeling immunohistochemical study. In: Histochemistry and Cell Biology. 2003 ; Vol. 119, No. 2. pp. 125-130.
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