Abstract
The generation of asymmetry in the one-cell embryo of Caenorhabditis elegans is necessary to establish the anterior-posterior axis and to ensure the proper identity of early blastomeres. Maternal-effect lethal mutations with a partitioning defective phenotype (par) have identified several genes involved in this process. We have identified a new gene, par-6, which acts in conjunction with other par genes to properly localize cytoplasmic components in the early embryo. The early phenotypes of par-6 embryos include the generation of equal-sized blastomeres, improper localization of P granules and SKN-1 protein, and abnormal second division cleavage patterns. Overall, this phenotype is very similar to that caused by mutations in a previously described gene, par-3. The probable basis for this similarity is revealed by our genetic and immunolocalization results; par-6 acts through par-3 by localizing or maintaining the PAR-3 protein at the cell periphery. In addition, we find that loss-of-function par-6 mutations act as dominant bypass suppressors of loss-of-function mutations in par-2.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3133-3140 |
| Number of pages | 8 |
| Journal | Development |
| Volume | 122 |
| Issue number | 10 |
| State | Published - 1996 |
| Externally published | Yes |
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Keywords
- Assymmetry
- Axis
- Caenorhabditis elegans
- Cell polarity
- Embryogenesis
- par-6
- Suppression
ASJC Scopus subject areas
- Anatomy
- Cell Biology
Cite this
par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3. / Watts, Jennifer L.; Etemad-Moghadam, Bijan; Guo, Su; Boyd, Lynn; Draper, Bruce W.; Mello, Craig C.; Priess, James R.; Kemphues, Kenneth J.
In: Development, Vol. 122, No. 10, 1996, p. 3133-3140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - par-6, a gene involved in the establishment of asymmetry in early C. elegans embryos, mediates the asymmetric localization of PAR-3
AU - Watts, Jennifer L.
AU - Etemad-Moghadam, Bijan
AU - Guo, Su
AU - Boyd, Lynn
AU - Draper, Bruce W.
AU - Mello, Craig C.
AU - Priess, James R.
AU - Kemphues, Kenneth J.
PY - 1996
Y1 - 1996
N2 - The generation of asymmetry in the one-cell embryo of Caenorhabditis elegans is necessary to establish the anterior-posterior axis and to ensure the proper identity of early blastomeres. Maternal-effect lethal mutations with a partitioning defective phenotype (par) have identified several genes involved in this process. We have identified a new gene, par-6, which acts in conjunction with other par genes to properly localize cytoplasmic components in the early embryo. The early phenotypes of par-6 embryos include the generation of equal-sized blastomeres, improper localization of P granules and SKN-1 protein, and abnormal second division cleavage patterns. Overall, this phenotype is very similar to that caused by mutations in a previously described gene, par-3. The probable basis for this similarity is revealed by our genetic and immunolocalization results; par-6 acts through par-3 by localizing or maintaining the PAR-3 protein at the cell periphery. In addition, we find that loss-of-function par-6 mutations act as dominant bypass suppressors of loss-of-function mutations in par-2.
AB - The generation of asymmetry in the one-cell embryo of Caenorhabditis elegans is necessary to establish the anterior-posterior axis and to ensure the proper identity of early blastomeres. Maternal-effect lethal mutations with a partitioning defective phenotype (par) have identified several genes involved in this process. We have identified a new gene, par-6, which acts in conjunction with other par genes to properly localize cytoplasmic components in the early embryo. The early phenotypes of par-6 embryos include the generation of equal-sized blastomeres, improper localization of P granules and SKN-1 protein, and abnormal second division cleavage patterns. Overall, this phenotype is very similar to that caused by mutations in a previously described gene, par-3. The probable basis for this similarity is revealed by our genetic and immunolocalization results; par-6 acts through par-3 by localizing or maintaining the PAR-3 protein at the cell periphery. In addition, we find that loss-of-function par-6 mutations act as dominant bypass suppressors of loss-of-function mutations in par-2.
KW - Assymmetry
KW - Axis
KW - Caenorhabditis elegans
KW - Cell polarity
KW - Embryogenesis
KW - par-6
KW - Suppression
UR - http://www.scopus.com/inward/record.url?scp=0029850113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029850113&partnerID=8YFLogxK
M3 - Article
C2 - 8898226
AN - SCOPUS:0029850113
VL - 122
SP - 3133
EP - 3140
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 10
ER -