Pancreatitis following the intraductal injection of partially purified enterokinase in dogs

James B. Hammond, Nirmal S Mann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Experimental pancreatitis (PT) is induced by proximal and distal duodenal closure in the bile-duct-ligated dog, by causing duodeno-pancreatic reflux of lumenal secretions. It has been postulated that trypsin and enterokinase (EK) in the secretions activate trypsinogen within the pancreas, producing PT. There is supporting evidence for trypsin, but EK has not previously been investigated. To determine whether EK alone could cause PT, we injected saline suspensions of partially purified EK, and other test materials, into the duct of Wirsung of dogs and after 24 hr examined their pancreases and estimated the increment in serum amylase. Following 0.5% EK, both PT and hyperamylasemia (HA) ensued; HA without PT occurred when EK was inactivated by heat, administered with trypsin inhibitor (TI), or administered in more dilute solution. Injection of TI or of hog gastric mucin likewise leads to HA but not to PT. It is concluded that the PT observed was due to EK activity, and that therefore EK could contribute to the production of PT in the closed-duodenalloop model. The HA observed in the absence of PT is unexplained but appears to be related to the colloidal properties of the materials injected.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalThe American Journal of Digestive Diseases
Volume22
Issue number3
DOIs
StatePublished - Mar 1977
Externally publishedYes

Fingerprint

Enteropeptidase
Pancreatitis
Dogs
Hyperamylasemia
Injections
Trypsin Inhibitors
Trypsin
Pancreas
Gastric Mucins
Trypsinogen
Pancreatic Ducts
Amylases
Bile Ducts
Suspensions
Hot Temperature

ASJC Scopus subject areas

  • Gastroenterology
  • Medicine(all)

Cite this

Pancreatitis following the intraductal injection of partially purified enterokinase in dogs. / Hammond, James B.; Mann, Nirmal S.

In: The American Journal of Digestive Diseases, Vol. 22, No. 3, 03.1977, p. 182-188.

Research output: Contribution to journalArticle

@article{674b718bbad14f26be5ba8604b9f4e9a,
title = "Pancreatitis following the intraductal injection of partially purified enterokinase in dogs",
abstract = "Experimental pancreatitis (PT) is induced by proximal and distal duodenal closure in the bile-duct-ligated dog, by causing duodeno-pancreatic reflux of lumenal secretions. It has been postulated that trypsin and enterokinase (EK) in the secretions activate trypsinogen within the pancreas, producing PT. There is supporting evidence for trypsin, but EK has not previously been investigated. To determine whether EK alone could cause PT, we injected saline suspensions of partially purified EK, and other test materials, into the duct of Wirsung of dogs and after 24 hr examined their pancreases and estimated the increment in serum amylase. Following 0.5{\%} EK, both PT and hyperamylasemia (HA) ensued; HA without PT occurred when EK was inactivated by heat, administered with trypsin inhibitor (TI), or administered in more dilute solution. Injection of TI or of hog gastric mucin likewise leads to HA but not to PT. It is concluded that the PT observed was due to EK activity, and that therefore EK could contribute to the production of PT in the closed-duodenalloop model. The HA observed in the absence of PT is unexplained but appears to be related to the colloidal properties of the materials injected.",
author = "Hammond, {James B.} and Mann, {Nirmal S}",
year = "1977",
month = "3",
doi = "10.1007/BF01072274",
language = "English (US)",
volume = "22",
pages = "182--188",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Pancreatitis following the intraductal injection of partially purified enterokinase in dogs

AU - Hammond, James B.

AU - Mann, Nirmal S

PY - 1977/3

Y1 - 1977/3

N2 - Experimental pancreatitis (PT) is induced by proximal and distal duodenal closure in the bile-duct-ligated dog, by causing duodeno-pancreatic reflux of lumenal secretions. It has been postulated that trypsin and enterokinase (EK) in the secretions activate trypsinogen within the pancreas, producing PT. There is supporting evidence for trypsin, but EK has not previously been investigated. To determine whether EK alone could cause PT, we injected saline suspensions of partially purified EK, and other test materials, into the duct of Wirsung of dogs and after 24 hr examined their pancreases and estimated the increment in serum amylase. Following 0.5% EK, both PT and hyperamylasemia (HA) ensued; HA without PT occurred when EK was inactivated by heat, administered with trypsin inhibitor (TI), or administered in more dilute solution. Injection of TI or of hog gastric mucin likewise leads to HA but not to PT. It is concluded that the PT observed was due to EK activity, and that therefore EK could contribute to the production of PT in the closed-duodenalloop model. The HA observed in the absence of PT is unexplained but appears to be related to the colloidal properties of the materials injected.

AB - Experimental pancreatitis (PT) is induced by proximal and distal duodenal closure in the bile-duct-ligated dog, by causing duodeno-pancreatic reflux of lumenal secretions. It has been postulated that trypsin and enterokinase (EK) in the secretions activate trypsinogen within the pancreas, producing PT. There is supporting evidence for trypsin, but EK has not previously been investigated. To determine whether EK alone could cause PT, we injected saline suspensions of partially purified EK, and other test materials, into the duct of Wirsung of dogs and after 24 hr examined their pancreases and estimated the increment in serum amylase. Following 0.5% EK, both PT and hyperamylasemia (HA) ensued; HA without PT occurred when EK was inactivated by heat, administered with trypsin inhibitor (TI), or administered in more dilute solution. Injection of TI or of hog gastric mucin likewise leads to HA but not to PT. It is concluded that the PT observed was due to EK activity, and that therefore EK could contribute to the production of PT in the closed-duodenalloop model. The HA observed in the absence of PT is unexplained but appears to be related to the colloidal properties of the materials injected.

UR - http://www.scopus.com/inward/record.url?scp=0017614713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017614713&partnerID=8YFLogxK

U2 - 10.1007/BF01072274

DO - 10.1007/BF01072274

M3 - Article

C2 - 842525

AN - SCOPUS:0017614713

VL - 22

SP - 182

EP - 188

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 3

ER -