Pancreatic sympathetic nerves contribute to increased glucagon secretion during severe hypoglycemia in dogs

Peter J Havel, Thomas O. Mundinger, Gerald J. Taborsky

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

To determine if pancreatic sympathetic nerves can contribute to increased glucagon secretion during hypoglycemia, plasma glucagon and pancreatic glucagon secretion in situ were measured before and during insulin-induced hypoglycemia in three groups of halothane-anesthetized dogs. All dogs were bilaterally vagotomized to eliminate the input from pancreatic parasympathetic nerves. One group of dogs received only vagotomy (VAGX). A second group was vagotomized and adrenalectomized (VAGX + ADX). A third group received vagotomy, adrenalectomy, plus surgical denervation of the pancreas (VAGX + ADX + NERVX) to prevent activation of pancreatic sympathetic nerves. In dogs with VAGX only, hypoglycemia increased plasma epinephrine (Epi), pancreatic norepinephrine (NE) output (+320 ± 140 pg/min, P < 0.05), arterial plasma glucagon (+28 ± 12 pg/ml, P < 0.01), and pancreatic glucagon output (+ 1,470 ± 370 pg/min, P < 0.01). The addition of ADX eliminated the increase of Epi but did not increase pancreatic NE output (+370 ± 190 pg/min P < 0.025), arterial plasma glucagon (+20 ± 5 pg/ml, P < 0.01), or pancreatic glucagon output (+ 810 ± 200 pg/min, P < 0.01). In contrast, the addition of pancreatic denervation eliminated the increase of pancreatic NE output (-20 ± 40 pg/min, P < 0.05 vs. VAGX), the arterial glucagon (+ 1 ± 2 pg/ml, P < 0.01 vs. VAGX), and pancreatic glucagon output responses (+210 ± 280 pg/min, P < 0.025 vs. VAGX) to hypoglycemia. Thus activation of pancreatic sympathetic nerves can contribute to the increased glucagon secretion during severe insulin-induced hypoglycemia in dogs.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume270
Issue number1 33-1
StatePublished - 1996

Fingerprint

Glucagon
Hypoglycemia
Dogs
Plasmas
Norepinephrine
Vagotomy
Denervation
Epinephrine
Chemical activation
Insulin
Adrenalectomy
Halothane
Pancreas

Keywords

  • adrenalectomy
  • epinephrine
  • norepinephrine
  • pancreatic denervation
  • vagotomy

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Pancreatic sympathetic nerves contribute to increased glucagon secretion during severe hypoglycemia in dogs. / Havel, Peter J; Mundinger, Thomas O.; Taborsky, Gerald J.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 270, No. 1 33-1, 1996.

Research output: Contribution to journalArticle

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abstract = "To determine if pancreatic sympathetic nerves can contribute to increased glucagon secretion during hypoglycemia, plasma glucagon and pancreatic glucagon secretion in situ were measured before and during insulin-induced hypoglycemia in three groups of halothane-anesthetized dogs. All dogs were bilaterally vagotomized to eliminate the input from pancreatic parasympathetic nerves. One group of dogs received only vagotomy (VAGX). A second group was vagotomized and adrenalectomized (VAGX + ADX). A third group received vagotomy, adrenalectomy, plus surgical denervation of the pancreas (VAGX + ADX + NERVX) to prevent activation of pancreatic sympathetic nerves. In dogs with VAGX only, hypoglycemia increased plasma epinephrine (Epi), pancreatic norepinephrine (NE) output (+320 ± 140 pg/min, P < 0.05), arterial plasma glucagon (+28 ± 12 pg/ml, P < 0.01), and pancreatic glucagon output (+ 1,470 ± 370 pg/min, P < 0.01). The addition of ADX eliminated the increase of Epi but did not increase pancreatic NE output (+370 ± 190 pg/min P < 0.025), arterial plasma glucagon (+20 ± 5 pg/ml, P < 0.01), or pancreatic glucagon output (+ 810 ± 200 pg/min, P < 0.01). In contrast, the addition of pancreatic denervation eliminated the increase of pancreatic NE output (-20 ± 40 pg/min, P < 0.05 vs. VAGX), the arterial glucagon (+ 1 ± 2 pg/ml, P < 0.01 vs. VAGX), and pancreatic glucagon output responses (+210 ± 280 pg/min, P < 0.025 vs. VAGX) to hypoglycemia. Thus activation of pancreatic sympathetic nerves can contribute to the increased glucagon secretion during severe insulin-induced hypoglycemia in dogs.",
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