Abstract
To determine if acute stress activates pancreatic noradrenergic nerves, pancreatic norepinehrine (NE) output (spillover) was measured in halothane-anesthetized dogs. Central neuroglucopenia, induced by intravenous 2-deoxy-D-glucose ([2-DG] 600 mg/kg + 13.5 mg/kg-1 per min-1) increased pancreatic NE output from a baseline of 380 ± 100 to 1,490 ± 340 pg/min (Δ = 1,110 ± 290 pg/min, P < 0.01). Surgical denervation of the pancreas reduced this response by 90% (Δ = +120 ± 50 pg/min, P < 0.01 vs. intact innervation), suggesting that 2-DG activated pancreatic nerves by increasing the central sympathetic outflow to the pancreas rather than by actin directly on nerves within the pancreas itself. These experiments provide the first direct evidence of stress-induced activation of pancreatic noradrenergic nerves in vivo. In contrast, neither hemorrhagic hypotension (50 mmHg) nor hypoxia (6-8% O2) increased pancreatic NE output (Δ = +80 ± 110 and -20 ± 60 pg/min, respectively, P < 0.01 vs. neuroglucopenia) despite both producing increases of arterial plasma NE and epinephrine similar to glucopenia. The activation of pancreatic noradrenergic nerves is thus stress specific. Furthermore, because both glucopenia and hypotension increased arterial NE, yet only glucopenia activated pancreatic nerves, it is suggested that a regionally selective pattern of sympathetic activation can be elicited by acute stress, a condition in which sympathetic activation has traditionally been thought to be generalized and nondiscrete.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1538-1545 |
| Number of pages | 8 |
| Journal | Journal of Clinical Investigation |
| Volume | 82 |
| Issue number | 5 |
| State | Published - 1988 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
Cite this
Pancreatic noradrenergic nerves are activated by neuroglucopenia but not by hypotension or hypoxia in the dog. Evidence for stress-specific and regionally selective activation of the sympathetic nervous system. / Havel, Peter J; Veith, R. C.; Dunning, B. E.; Taborsky, G. J.
In: Journal of Clinical Investigation, Vol. 82, No. 5, 1988, p. 1538-1545.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pancreatic noradrenergic nerves are activated by neuroglucopenia but not by hypotension or hypoxia in the dog. Evidence for stress-specific and regionally selective activation of the sympathetic nervous system
AU - Havel, Peter J
AU - Veith, R. C.
AU - Dunning, B. E.
AU - Taborsky, G. J.
PY - 1988
Y1 - 1988
N2 - To determine if acute stress activates pancreatic noradrenergic nerves, pancreatic norepinehrine (NE) output (spillover) was measured in halothane-anesthetized dogs. Central neuroglucopenia, induced by intravenous 2-deoxy-D-glucose ([2-DG] 600 mg/kg + 13.5 mg/kg-1 per min-1) increased pancreatic NE output from a baseline of 380 ± 100 to 1,490 ± 340 pg/min (Δ = 1,110 ± 290 pg/min, P < 0.01). Surgical denervation of the pancreas reduced this response by 90% (Δ = +120 ± 50 pg/min, P < 0.01 vs. intact innervation), suggesting that 2-DG activated pancreatic nerves by increasing the central sympathetic outflow to the pancreas rather than by actin directly on nerves within the pancreas itself. These experiments provide the first direct evidence of stress-induced activation of pancreatic noradrenergic nerves in vivo. In contrast, neither hemorrhagic hypotension (50 mmHg) nor hypoxia (6-8% O2) increased pancreatic NE output (Δ = +80 ± 110 and -20 ± 60 pg/min, respectively, P < 0.01 vs. neuroglucopenia) despite both producing increases of arterial plasma NE and epinephrine similar to glucopenia. The activation of pancreatic noradrenergic nerves is thus stress specific. Furthermore, because both glucopenia and hypotension increased arterial NE, yet only glucopenia activated pancreatic nerves, it is suggested that a regionally selective pattern of sympathetic activation can be elicited by acute stress, a condition in which sympathetic activation has traditionally been thought to be generalized and nondiscrete.
AB - To determine if acute stress activates pancreatic noradrenergic nerves, pancreatic norepinehrine (NE) output (spillover) was measured in halothane-anesthetized dogs. Central neuroglucopenia, induced by intravenous 2-deoxy-D-glucose ([2-DG] 600 mg/kg + 13.5 mg/kg-1 per min-1) increased pancreatic NE output from a baseline of 380 ± 100 to 1,490 ± 340 pg/min (Δ = 1,110 ± 290 pg/min, P < 0.01). Surgical denervation of the pancreas reduced this response by 90% (Δ = +120 ± 50 pg/min, P < 0.01 vs. intact innervation), suggesting that 2-DG activated pancreatic nerves by increasing the central sympathetic outflow to the pancreas rather than by actin directly on nerves within the pancreas itself. These experiments provide the first direct evidence of stress-induced activation of pancreatic noradrenergic nerves in vivo. In contrast, neither hemorrhagic hypotension (50 mmHg) nor hypoxia (6-8% O2) increased pancreatic NE output (Δ = +80 ± 110 and -20 ± 60 pg/min, respectively, P < 0.01 vs. neuroglucopenia) despite both producing increases of arterial plasma NE and epinephrine similar to glucopenia. The activation of pancreatic noradrenergic nerves is thus stress specific. Furthermore, because both glucopenia and hypotension increased arterial NE, yet only glucopenia activated pancreatic nerves, it is suggested that a regionally selective pattern of sympathetic activation can be elicited by acute stress, a condition in which sympathetic activation has traditionally been thought to be generalized and nondiscrete.
UR - http://www.scopus.com/inward/record.url?scp=0024253725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024253725&partnerID=8YFLogxK
M3 - Article
C2 - 3183052
AN - SCOPUS:0024253725
VL - 82
SP - 1538
EP - 1545
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 5
ER -