Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices

Dominique Debanne, Sami Boudkkazi, Emilie Campanac, Robert H. Cudmore, Pierre Giraud, Laure Fronzaroli-Molinieres, Edmond Carlier, Olivier Caillard

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Analysis of synaptic transmission, synaptic plasticity, axonal processing, synaptic timing or electrical coupling requires the simultaneous recording of both the pre- and postsynaptic compartments. Paired-recording technique of monosynaptically connected neurons is also an appropriate technique to probe the function of small molecules (calcium buffers, peptides or small proteins) at presynaptic terminals that are too small to allow direct whole-cell patch-clamp recording. We describe here a protocol for obtaining, in acute and cultured slices, synaptically connected pairs of cortical and hippocampal neurons, with a reasonably high probability. The protocol includes four main stages (acute/cultured slice preparation, visualization, recording and analysis) and can be completed in ∼4 h.

Original languageEnglish (US)
Pages (from-to)1559-1568
Number of pages10
JournalNature Protocols
Volume3
Issue number10
DOIs
StatePublished - Oct 3 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Debanne, D., Boudkkazi, S., Campanac, E., Cudmore, R. H., Giraud, P., Fronzaroli-Molinieres, L., Carlier, E., & Caillard, O. (2008). Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices. Nature Protocols, 3(10), 1559-1568. https://doi.org/10.1038/nprot.2008.147