PAF- and bradykinin-induced hyperpermeability of rat venules is independent of actin-myosin contraction

R. H. Adamson, M. Zeng, G. N. Adamson, J. F. Lenz, F. E. Curry

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


We tested the hypothesis that acutely induced hyperpermeability is dependent on actin-myosin contractility by using individually perfused mesentery venules of pentobarbital-anesthetized rats. Venule hydraulic conductivity (Lp) was measured to monitor hyperpermeability response to the platelet-activating factor (PAF) 1-O-hexadecyl-2-acetylsn-glycero-3-phosphocholine or bradykinin. Perfusion with PAF (10 nM) induced a robust transient high Lp [24.3 ± 1.7 × 10-7 cm/(s·cmH2O)] that peaked in 8.9 ± 0.5 min and then returned toward control Lp [1.6 ± 0.1 × 10-7 cm/(s·cmH2O)]. Reconstruction of venular segments with the use of transmission electron microscopy of serial sections confirmed that PAF induces paracellular inflammatory gaps. Specific inhibition of myosin light chain kinase (MLCK) with 1-10 μM 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride (ML-7) failed to block the PAF Lp response or change the time-to-peak Lp. ML-7 reduced baseline Lp 50% at 40 min of pretreatment. ML-7 also increased the rate of recovery from PAF hyperpermeability measured as the decrease of half-time of recovery from 4.8 ± 0.7 to 3.2 ± 0.3 min. Inhibition of myosin ATPase with 5-20 mM 2,3-butanedione 2-monoxime also failed to alter the hyperpermeability response to PAF. Similar results were found using ML-7 to modulate responses. These experiments indicate that an actin-myosin contractile mechanism modulated by MLCK does not contribute significantly to the robust initial increase in permeability of rat venular microvessels exposed to two common inflammatory mediators. The results are consistent with paracellular gap formation by local release of endothelial-endothelial cell adhesion structures in the absence of contraction by the actin-myosin network.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number1 54-1
StatePublished - Jul 1 2003


  • Bradykinin
  • Inflammatory gaps
  • ML-7
  • Myosin light chain kinase
  • Platelet-activating factor
  • Vascular endothelium

ASJC Scopus subject areas

  • Physiology


Dive into the research topics of 'PAF- and bradykinin-induced hyperpermeability of rat venules is independent of actin-myosin contraction'. Together they form a unique fingerprint.

Cite this