Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines

Chun Wang, Jun Yuan, Hua Xun Wu, Yan Chang, Qingtong Wang, Yu Jing Wu, Li Hua Liu, Wei Wei

Research output: Contribution to journalArticle

44 Scopus citations


Objective: Paeoniflorin (Pae) was previously reported to inhibit inflammation in the skin of mice with allergic contact dermatitis (ACD); however, the mechanism remains unclear. The primary purpose of this study was to investigate the effect of Pae on the regulation of cytokine production in a murine model of ACD. Methods: ACD was induced in the mice by repeated application of dinitrochlorobenzene (DNCB) to their skin. Cutaneous inflammation was evaluated by measuring ear swelling and by histological examination. The cytokine levels were measured by enzyme-linked immunosorbent assays. Results: The results showed that topical application of DNCB caused obvious swelling and inflammatory cell infiltration. Treatment with Pae (70 or 140 mg/kg/d) significantly inhibited the cutaneous inflammation and decreased thymocyte proliferation in the mice with ACD. Additional data indicated that Pae increased interleukin-4 (IL-4) and IL-10 production but reduced IL-2 and IL-17 levels in the serum as well as in thymocyte and splenocyte culture supernatants. As expected, IL-2 and IL-17 levels in the serum displayed a significant positive correlation with the severity of skin inflammation. In contrast, IL-4 and IL-10 levels were negatively correlated with the inflammation. Conclusions: The anti-inflammatory action of Pae in the murine model of ACD may be related to its regulation of an imbalanced cytokine production.

Original languageEnglish (US)
Pages (from-to)1035-1044
Number of pages10
JournalInflammation Research
Issue number12
StatePublished - Dec 1 2013
Externally publishedYes



  • Allergic contact dermatitis
  • Interleukin -17
  • Interleukin-10
  • Interleukin-2
  • Interleukin-4
  • Paeoniflorin

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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