PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization

Lei Wan, Sean S. Molloy, Laurel Thomas, Gseping Liu, Yang Kevin Xiang, Sheree Lynn Rybak, Gary Thomas

Research output: Contribution to journalArticle

287 Scopus citations

Abstract

We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS- 1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell- free assays indicate TGN localization of furin is directed by a PACS-1- mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.

Original languageEnglish (US)
Pages (from-to)205-216
Number of pages12
JournalCell
Volume94
Issue number2
DOIs
StatePublished - Jul 24 1998
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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