PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization

Lei Wan, Sean S. Molloy, Laurel Thomas, Gseping Liu, Yang Kevin Xiang, Sheree Lynn Rybak, Gary Thomas

Research output: Contribution to journalArticle

282 Citations (Scopus)

Abstract

We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS- 1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell- free assays indicate TGN localization of furin is directed by a PACS-1- mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.

Original languageEnglish (US)
Pages (from-to)205-216
Number of pages12
JournalCell
Volume94
Issue number2
DOIs
StatePublished - Jul 24 1998
Externally publishedYes

Fingerprint

trans-Golgi Network
Picture archiving and communication systems
Furin
Protein Transport
Sorting
Genes
Proteins
Membrane Proteins
IGF Type 2 Receptor
Clathrin
Capsid Proteins
Machinery
Assays
Peptide Hydrolases
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. / Wan, Lei; Molloy, Sean S.; Thomas, Laurel; Liu, Gseping; Xiang, Yang Kevin; Rybak, Sheree Lynn; Thomas, Gary.

In: Cell, Vol. 94, No. 2, 24.07.1998, p. 205-216.

Research output: Contribution to journalArticle

Wan, Lei ; Molloy, Sean S. ; Thomas, Laurel ; Liu, Gseping ; Xiang, Yang Kevin ; Rybak, Sheree Lynn ; Thomas, Gary. / PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. In: Cell. 1998 ; Vol. 94, No. 2. pp. 205-216.
@article{c3ea08e6598d4669a0e1d87e76944f98,
title = "PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization",
abstract = "We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS- 1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell- free assays indicate TGN localization of furin is directed by a PACS-1- mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.",
author = "Lei Wan and Molloy, {Sean S.} and Laurel Thomas and Gseping Liu and Xiang, {Yang Kevin} and Rybak, {Sheree Lynn} and Gary Thomas",
year = "1998",
month = "7",
day = "24",
doi = "10.1016/S0092-8674(00)81420-8",
language = "English (US)",
volume = "94",
pages = "205--216",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization

AU - Wan, Lei

AU - Molloy, Sean S.

AU - Thomas, Laurel

AU - Liu, Gseping

AU - Xiang, Yang Kevin

AU - Rybak, Sheree Lynn

AU - Thomas, Gary

PY - 1998/7/24

Y1 - 1998/7/24

N2 - We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS- 1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell- free assays indicate TGN localization of furin is directed by a PACS-1- mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.

AB - We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, is strictly dependent on PACS- 1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell- free assays indicate TGN localization of furin is directed by a PACS-1- mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.

UR - http://www.scopus.com/inward/record.url?scp=0032563306&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032563306&partnerID=8YFLogxK

U2 - 10.1016/S0092-8674(00)81420-8

DO - 10.1016/S0092-8674(00)81420-8

M3 - Article

C2 - 9695949

AN - SCOPUS:0032563306

VL - 94

SP - 205

EP - 216

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -