Abstract
Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1111-1117 |
| Number of pages | 7 |
| Journal | Peptides |
| Volume | 22 |
| Issue number | 7 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Alternative splicing variants
- cAMP/protein kinase A cascades
- Cytosolic Ca concentration
- Development
- G-protein-coupled receptor
- Neural fold
- PACAP
- Phospholipase C/protein kinase C cascade
- Primitive streak stage
- Rat
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells. / Zhou, Chengji; Yada, Toshihiko; Kohno, Daisuke; Kikuyama, Sakae; Suzuki, Ryusuke; Mizushima, Hidekatsu; Shioda, Seiji.
In: Peptides, Vol. 22, No. 7, 2001, p. 1111-1117.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells
AU - Zhou, Chengji
AU - Yada, Toshihiko
AU - Kohno, Daisuke
AU - Kikuyama, Sakae
AU - Suzuki, Ryusuke
AU - Mizushima, Hidekatsu
AU - Shioda, Seiji
PY - 2001
Y1 - 2001
N2 - Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.
AB - Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.
KW - Alternative splicing variants
KW - cAMP/protein kinase A cascades
KW - Cytosolic Ca concentration
KW - Development
KW - G-protein-coupled receptor
KW - Neural fold
KW - PACAP
KW - Phospholipase C/protein kinase C cascade
KW - Primitive streak stage
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0034964632&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034964632&partnerID=8YFLogxK
U2 - 10.1016/S0196-9781(01)00437-5
DO - 10.1016/S0196-9781(01)00437-5
M3 - Article
VL - 22
SP - 1111
EP - 1117
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 7
ER -