Abstract
Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.
Original language | English (US) |
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Pages (from-to) | 1111-1117 |
Number of pages | 7 |
Journal | Peptides |
Volume | 22 |
Issue number | 7 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Alternative splicing variants
- cAMP/protein kinase A cascades
- Cytosolic Ca concentration
- Development
- G-protein-coupled receptor
- Neural fold
- PACAP
- Phospholipase C/protein kinase C cascade
- Primitive streak stage
- Rat
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience