PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells

Chengji Zhou; Toshihiko Yada; Daisuke Kohno; Sakae Kikuyama; Ryusuke Suzuki; Hidekatsu Mizushima; Seiji Shioda

doi:10.1016/S0196-9781(01)00437-5

PACAP activates PKA, PKC and Ca²⁺ signaling cascades in rat neuroepithelial cells

Chengji Zhou, Toshihiko Yada, Daisuke Kohno, Sakae Kikuyama, Ryusuke Suzuki, Hidekatsu Mizushima, Seiji Shioda

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Several studies have reported that the PAC₁ receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca²⁺ concentration ([Ca²⁺]_i) was increased by PACAP, but not VIP, in a concentration range from 10^-12 to 10^-8 M via the PAC₁-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC₁-R is linked to [Ca²⁺]_i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.

Original language	English (US)
Pages (from-to)	1111-1117
Number of pages	7
Journal	Peptides
Volume	22
Issue number	7
DOIs	https://doi.org/10.1016/S0196-9781(01)00437-5
State	Published - 2001
Externally published	Yes

Keywords

Alternative splicing variants
cAMP/protein kinase A cascades
Cytosolic Ca concentration
Development
G-protein-coupled receptor
Neural fold
PACAP
Phospholipase C/protein kinase C cascade
Primitive streak stage
Rat

ASJC Scopus subject areas

Biochemistry
Endocrinology
Physiology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S0196-9781(01)00437-5

Cite this

@article{c76e813ebe7e443abb992c8982ef22b7,

title = "PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells",

abstract = "Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.",

keywords = "Alternative splicing variants, cAMP/protein kinase A cascades, Cytosolic Ca concentration, Development, G-protein-coupled receptor, Neural fold, PACAP, Phospholipase C/protein kinase C cascade, Primitive streak stage, Rat",

author = "Chengji Zhou and Toshihiko Yada and Daisuke Kohno and Sakae Kikuyama and Ryusuke Suzuki and Hidekatsu Mizushima and Seiji Shioda",

year = "2001",

doi = "10.1016/S0196-9781(01)00437-5",

language = "English (US)",

volume = "22",

pages = "1111--1117",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "7",

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TY - JOUR

T1 - PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells

AU - Zhou, Chengji

AU - Yada, Toshihiko

AU - Kohno, Daisuke

AU - Kikuyama, Sakae

AU - Suzuki, Ryusuke

AU - Mizushima, Hidekatsu

AU - Shioda, Seiji

PY - 2001

Y1 - 2001

N2 - Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.

AB - Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.

KW - Alternative splicing variants

KW - cAMP/protein kinase A cascades

KW - Cytosolic Ca concentration

KW - Development

KW - G-protein-coupled receptor

KW - Neural fold

KW - PACAP

KW - Phospholipase C/protein kinase C cascade

KW - Primitive streak stage

KW - Rat

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DO - 10.1016/S0196-9781(01)00437-5

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AN - SCOPUS:0034964632

VL - 22

SP - 1111

EP - 1117

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 7

ER -