PACAP activates PKA, PKC and Ca2+ signaling cascades in rat neuroepithelial cells

Chengji Zhou, Toshihiko Yada, Daisuke Kohno, Sakae Kikuyama, Ryusuke Suzuki, Hidekatsu Mizushima, Seiji Shioda

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Several studies have reported that the PAC1 receptor (PAC1-R), the specific receptor for PACAP, is expressed at early developmental stages. Here, we describe that the cytosolic Ca2+ concentration ([Ca2+]i) was increased by PACAP, but not VIP, in a concentration range from 10-12 to 10-8 M via the PAC1-R in isolated single cells from the rat neural fold. This activation of the cells by PACAP was mimicked by agonists and inhibited by antagonists of the cAMP/PKA and PLC/PKC cascades. These data indicate that PACAP/PAC1-R is linked to [Ca2+]i signaling via two G-protein-coupled protein kinase pathways and may thereby play an important role in early neurodevelopment.

Original languageEnglish (US)
Pages (from-to)1111-1117
Number of pages7
Issue number7
StatePublished - 2001
Externally publishedYes


  • Alternative splicing variants
  • cAMP/protein kinase A cascades
  • Cytosolic Ca concentration
  • Development
  • G-protein-coupled receptor
  • Neural fold
  • Phospholipase C/protein kinase C cascade
  • Primitive streak stage
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience


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