P-selectin mediates neutrophil adhesion to endothelial cell borders

Alan R. Burns, Robert A. Bowden, Yasunori Abe, David C. Walker, Scott I. Simon, Mark L. Entman, C. Wayne Smith

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

During an acute inflammatory response, endothelial P-selectin (CD62P) can mediate the initial capture of neutrophils from the free flowing bloodstream. P-selectin is stored in secretory granules (Weibel-Palade bodies) and is rapidly expressed on the endothelial surface after stimulation with histamine or thrombin. Because neutrophil transmigration occurs preferentially at endothelial borders, we wished to determine whether P- selectin-dependent neutrophil capture (adhesion) occurs at endothelial cell borders. Under static or hydrodynamic flow (2 dyn/cm2) conditions, histamine (10-4 M) or thrombin (0.2 U/mL) treatment induced preferential (≥75%) neutrophil adhesion to the cell borders of endothelial monolayers. Blocking antibody studies established that neutrophil adhesion was completely P- selectin dependent. P-selectin surface expression increased significantly after histamine treatment and P-selectin immunostaining was concentrated along endothelial borders. We conclude that preferential P-selectin expression along endothelial borders may be an important mechanism for targeting neutrophil migration at endothelial borders.

Original languageEnglish (US)
Pages (from-to)299-306
Number of pages8
JournalJournal of Leukocyte Biology
Volume65
Issue number3
StatePublished - 1999
Externally publishedYes

Keywords

  • Adhesion molecules
  • Histamine
  • Inflammation
  • Leukocytes

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'P-selectin mediates neutrophil adhesion to endothelial cell borders'. Together they form a unique fingerprint.

Cite this