Abstract
During an acute inflammatory response, endothelial P-selectin (CD62P) can mediate the initial capture of neutrophils from the free flowing bloodstream. P-selectin is stored in secretory granules (Weibel-Palade bodies) and is rapidly expressed on the endothelial surface after stimulation with histamine or thrombin. Because neutrophil transmigration occurs preferentially at endothelial borders, we wished to determine whether P- selectin-dependent neutrophil capture (adhesion) occurs at endothelial cell borders. Under static or hydrodynamic flow (2 dyn/cm2) conditions, histamine (10-4 M) or thrombin (0.2 U/mL) treatment induced preferential (≥75%) neutrophil adhesion to the cell borders of endothelial monolayers. Blocking antibody studies established that neutrophil adhesion was completely P- selectin dependent. P-selectin surface expression increased significantly after histamine treatment and P-selectin immunostaining was concentrated along endothelial borders. We conclude that preferential P-selectin expression along endothelial borders may be an important mechanism for targeting neutrophil migration at endothelial borders.
Original language | English (US) |
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Pages (from-to) | 299-306 |
Number of pages | 8 |
Journal | Journal of Leukocyte Biology |
Volume | 65 |
Issue number | 3 |
State | Published - 1999 |
Externally published | Yes |
Keywords
- Adhesion molecules
- Histamine
- Inflammation
- Leukocytes
ASJC Scopus subject areas
- Cell Biology