Ozone-induced enhancement of airway hyperreactivity in rhesus macaques: Effects of antioxidant treatment

Cameron H. Flayer, Erik D. Larson, Anjali Joseph, Sean Kao, Wenxiu Qu, Austin Van Haren, Christopher M. Royer, Lisa A. Miller, John P. Capitanio, Thais Sielecki, Melpo Christofidou-Solomidou, Angela Haczku

Research output: Contribution to journalArticle

Abstract

Background: Ozone (O3) inhalation elicits airway inflammation and impairs treatment responsiveness in asthmatic patients. The underlying immune mechanisms have been difficult to study because of the lack of relevant experimental models. Rhesus macaques spontaneously have asthma and have a similar immune system to human subjects. Objectives: We sought to investigate mucosal immune changes after O3 inhalation in a clinically relevant nonhuman primate asthma model and to study the effects of an antioxidant synthetic lignan (synthetic secoisolariciresinol diglucoside [LGM2605]). Methods: A cohort of macaques (n = 17) previously characterized with airway hyperreactivity (AHR) to methacholine was assessed (day 1). Macaques were treated (orally) with LGM2605 (25 mg/kg) or placebo twice per day for 7 days, exposed to 0.3 ppm O3 or air for 6 hours (on day 7), and studied 12 hours later (day 8). Lung function, blood and bronchoalveolar lavage (BAL) fluid immune cell profile, and bronchial brushing and blood cell mRNA expression were assessed. Results: O3 induced significant BAL fluid neutrophilia and eosinophilia and increased AHR and expression of IL6 and IL25 mRNA in the airway epithelium together with increased BAL fluid group 2 innate lymphoid cell (ILC2s), CD1c+ myeloid dendritic cell, and CD4+ T-cell counts and diminished surfactant protein D expression. Although LGM2605 attenuated some of the immune and inflammatory changes, it completely abolished O3-induced AHR. Conclusion: ILC2s, CD1c+ myeloid dendritic cells, and CD4+ T cells are selectively involved in O3-induced asthma exacerbation. The inflammatory changes were partially prevented by antioxidant pretreatment with LGM2605, which had an unexpectedly disproportionate protective effect on AHR.

Original languageEnglish (US)
JournalJournal of Allergy and Clinical Immunology
DOIs
StateAccepted/In press - Jan 1 2019

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Ozone
Bronchoalveolar Lavage Fluid
Macaca mulatta
Antioxidants
Asthma
Macaca
Myeloid Cells
Dendritic Cells
Inhalation
Pulmonary Surfactant-Associated Protein D
T-Lymphocytes
Lignans
Messenger RNA
Methacholine Chloride
Eosinophilia
Therapeutics
CD4 Lymphocyte Count
Primates
Immune System
Interleukin-6

Keywords

  • air pollution
  • airway inflammation
  • Asthma
  • nonhuman primate
  • therapies (investigational)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ozone-induced enhancement of airway hyperreactivity in rhesus macaques : Effects of antioxidant treatment. / Flayer, Cameron H.; Larson, Erik D.; Joseph, Anjali; Kao, Sean; Qu, Wenxiu; Van Haren, Austin; Royer, Christopher M.; Miller, Lisa A.; Capitanio, John P.; Sielecki, Thais; Christofidou-Solomidou, Melpo; Haczku, Angela.

In: Journal of Allergy and Clinical Immunology, 01.01.2019.

Research output: Contribution to journalArticle

Flayer, Cameron H. ; Larson, Erik D. ; Joseph, Anjali ; Kao, Sean ; Qu, Wenxiu ; Van Haren, Austin ; Royer, Christopher M. ; Miller, Lisa A. ; Capitanio, John P. ; Sielecki, Thais ; Christofidou-Solomidou, Melpo ; Haczku, Angela. / Ozone-induced enhancement of airway hyperreactivity in rhesus macaques : Effects of antioxidant treatment. In: Journal of Allergy and Clinical Immunology. 2019.
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abstract = "Background: Ozone (O3) inhalation elicits airway inflammation and impairs treatment responsiveness in asthmatic patients. The underlying immune mechanisms have been difficult to study because of the lack of relevant experimental models. Rhesus macaques spontaneously have asthma and have a similar immune system to human subjects. Objectives: We sought to investigate mucosal immune changes after O3 inhalation in a clinically relevant nonhuman primate asthma model and to study the effects of an antioxidant synthetic lignan (synthetic secoisolariciresinol diglucoside [LGM2605]). Methods: A cohort of macaques (n = 17) previously characterized with airway hyperreactivity (AHR) to methacholine was assessed (day 1). Macaques were treated (orally) with LGM2605 (25 mg/kg) or placebo twice per day for 7 days, exposed to 0.3 ppm O3 or air for 6 hours (on day 7), and studied 12 hours later (day 8). Lung function, blood and bronchoalveolar lavage (BAL) fluid immune cell profile, and bronchial brushing and blood cell mRNA expression were assessed. Results: O3 induced significant BAL fluid neutrophilia and eosinophilia and increased AHR and expression of IL6 and IL25 mRNA in the airway epithelium together with increased BAL fluid group 2 innate lymphoid cell (ILC2s), CD1c+ myeloid dendritic cell, and CD4+ T-cell counts and diminished surfactant protein D expression. Although LGM2605 attenuated some of the immune and inflammatory changes, it completely abolished O3-induced AHR. Conclusion: ILC2s, CD1c+ myeloid dendritic cells, and CD4+ T cells are selectively involved in O3-induced asthma exacerbation. The inflammatory changes were partially prevented by antioxidant pretreatment with LGM2605, which had an unexpectedly disproportionate protective effect on AHR.",
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AU - Kao, Sean

AU - Qu, Wenxiu

AU - Van Haren, Austin

AU - Royer, Christopher M.

AU - Miller, Lisa A.

AU - Capitanio, John P.

AU - Sielecki, Thais

AU - Christofidou-Solomidou, Melpo

AU - Haczku, Angela

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