Abstract
Open-chest biopsies of lung were performed on three cynomolgus monkeys (Macaca fascicularis) 4-6 weeks before and immediately after exposure to 1.5 ppm of ozone for 1 week. Two control monkeys were subjected to comparable procedures, but were exposed to filtered air for 1 week immediately prior to the second biopsy. Collagen synthesis rate of each lung biopsy specimen was determined. Enhanced rates of collagen synthesis were observed in the lung biopsy specimens obtained from the monkeys exposed to ozone; elevated rates of collagen synthesis were not observed in lung biopsy specimens from the two monkeys undergoing identical surgical procedures but not exposed to ozone. We conclude that exposures of monkeys to high levels of ozone caused increased rates of collagen synthesis in their lungs, suggesting that the ozone-induced fibrotic changes observed previously in rat lungs also occur in lungs anatomically similar to those of humans. This experimental design, in which each animal serves as its own control, is a practical way to perform such exposure experiments using small numbers of valuable animals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 579-585 |
| Number of pages | 7 |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 30 1981 |
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ASJC Scopus subject areas
- Pharmacology
- Toxicology
Cite this
Ozone-induced alterations in collagen metabolism of monkey lungs : Use of biopsy-obtained lung tissue. / Last, Jerold A; Hesterberg, Thomas W.; Reiser, Karen M.; Cross, Carroll E; Amis, Terence C.; Gunn, Carolyn; Steffey, Eugene; Grandy, Jacqueline; Henrickson, Roy.
In: Toxicology and Applied Pharmacology, Vol. 60, No. 3, 30.09.1981, p. 579-585.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ozone-induced alterations in collagen metabolism of monkey lungs
T2 - Use of biopsy-obtained lung tissue
AU - Last, Jerold A
AU - Hesterberg, Thomas W.
AU - Reiser, Karen M.
AU - Cross, Carroll E
AU - Amis, Terence C.
AU - Gunn, Carolyn
AU - Steffey, Eugene
AU - Grandy, Jacqueline
AU - Henrickson, Roy
PY - 1981/9/30
Y1 - 1981/9/30
N2 - Open-chest biopsies of lung were performed on three cynomolgus monkeys (Macaca fascicularis) 4-6 weeks before and immediately after exposure to 1.5 ppm of ozone for 1 week. Two control monkeys were subjected to comparable procedures, but were exposed to filtered air for 1 week immediately prior to the second biopsy. Collagen synthesis rate of each lung biopsy specimen was determined. Enhanced rates of collagen synthesis were observed in the lung biopsy specimens obtained from the monkeys exposed to ozone; elevated rates of collagen synthesis were not observed in lung biopsy specimens from the two monkeys undergoing identical surgical procedures but not exposed to ozone. We conclude that exposures of monkeys to high levels of ozone caused increased rates of collagen synthesis in their lungs, suggesting that the ozone-induced fibrotic changes observed previously in rat lungs also occur in lungs anatomically similar to those of humans. This experimental design, in which each animal serves as its own control, is a practical way to perform such exposure experiments using small numbers of valuable animals.
AB - Open-chest biopsies of lung were performed on three cynomolgus monkeys (Macaca fascicularis) 4-6 weeks before and immediately after exposure to 1.5 ppm of ozone for 1 week. Two control monkeys were subjected to comparable procedures, but were exposed to filtered air for 1 week immediately prior to the second biopsy. Collagen synthesis rate of each lung biopsy specimen was determined. Enhanced rates of collagen synthesis were observed in the lung biopsy specimens obtained from the monkeys exposed to ozone; elevated rates of collagen synthesis were not observed in lung biopsy specimens from the two monkeys undergoing identical surgical procedures but not exposed to ozone. We conclude that exposures of monkeys to high levels of ozone caused increased rates of collagen synthesis in their lungs, suggesting that the ozone-induced fibrotic changes observed previously in rat lungs also occur in lungs anatomically similar to those of humans. This experimental design, in which each animal serves as its own control, is a practical way to perform such exposure experiments using small numbers of valuable animals.
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U2 - 10.1016/0041-008X(81)90345-8
DO - 10.1016/0041-008X(81)90345-8
M3 - Article
VL - 60
SP - 579
EP - 585
JO - Toxicology and Applied Pharmacology
JF - Toxicology and Applied Pharmacology
SN - 0041-008X
IS - 3
ER -