Ozone-induced adaptive and reactive cellular changes in respiratory bronchioles of bonnet monkeys

R. K. Moffatt, D. M. Hyde, Charles Plopper, W. S. Tyler, L. F. Putney

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

To characterize the response of respiratory bronchioles (RBs) to chronic high ambient levels of ozone, bonnet monkeys were exposed for 90 days to 0, 0.4, or 0.64 ppm ozone (UV photometric standard; 3 monkeys/exposure). Morphologic changes in respiratory bronchiolar epithelium and interstitium were evaluated quantitatively at both the light and transmission electron microscopic levels. Significant changes in respiratory bronchioles following exposure included: (1) a thicker wall and a narrower lumen, (2) a thicker epithelial compartment and a much thicker interstitial compartment, (3) shifts in epithelial cell populations with many more nonciliated bronchiolar epithelial cells and fewer squamous type I epithelial cells, (4) larger nonciliated bronchiolar epithelial cells with a larger compliment of cellular organelles associated with protein synthesis, (5) greater amounts of both interstitial fibers and amorphous ground substance, (6) greater numbers of interstitial smooth muscle cells per epithelial basal lamina surface area, and (7) greater volumes of interstitial smooth muscle, macrophages, mast cells, and neutrophils per epithelial basal lamina surface area. These observations imply that chronic ozone exposure causes a concentration-dependent reactive peribronchiolar inflammatory response and an adaptive response consisting of hypertrophy and hyperplasia of the nonciliated bronchiolar cell.

Original languageEnglish (US)
Pages (from-to)57-74
Number of pages18
JournalExperimental Lung Research
Volume12
Issue number1
DOIs
StatePublished - 1987

Fingerprint

Macaca radiata
Bronchioles
Ozone
Epithelial Cells
Basement Membrane
Muscle
Respiratory Mucosa
Macrophages
Mast Cells
Organelles
Hypertrophy
Smooth Muscle Myocytes
Hyperplasia
Haplorhini
Smooth Muscle
Neutrophils
Cells
Electrons
Light
Fibers

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Ozone-induced adaptive and reactive cellular changes in respiratory bronchioles of bonnet monkeys. / Moffatt, R. K.; Hyde, D. M.; Plopper, Charles; Tyler, W. S.; Putney, L. F.

In: Experimental Lung Research, Vol. 12, No. 1, 1987, p. 57-74.

Research output: Contribution to journalArticle

Moffatt, R. K. ; Hyde, D. M. ; Plopper, Charles ; Tyler, W. S. ; Putney, L. F. / Ozone-induced adaptive and reactive cellular changes in respiratory bronchioles of bonnet monkeys. In: Experimental Lung Research. 1987 ; Vol. 12, No. 1. pp. 57-74.
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