Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum

Jens J. Thiele, Maret G. Traber, Thomas G. Polefka, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The presence of ozone (O3) in photochemical smog is an important health concern. We hypothesized that the stratum corneum (SC), as the outermost skin layer and the permeability barrier of the skin, represents a sensitive target for O3-induced oxidative stress. To test this hypothesis, SKH-1 hairless mice were anesthetized and exposed for 2 h to O3 by using two strategies: (i) single exposures to 0 (n = 12), 1 (n = 4), 5 (n = 4), and 10 (n = 4) ppm; and (ii) repeated daily exposures to 0 ppm (controls; n = 4) and 1 ppm (n = 4) for six consecutive days. New techniques based on the removal of SC by tape stripping were used to analyze the biologic effects of O3 with respect to vitamin E depletion and lipid peroxidation. SC tissue was extracted from the tape and immediately analyzed by HPLC for vitamin E and malondialdehyde (MDA) concentrations. After in vivo exposure to increasing O3 doses, vitamin E was depleted and MDA formation was increased, both in a dose-dependent manner. Remarkably, repeated low-level O3 exposures resulted in cumulative oxidative effects in the SC: As compared with O3 exposures of 0 ppm (α-tocopherol, 8.95 ± 1.3 pmol per mg; γ-tocopherol, 3.00 ± 0.3 pmol per mg; MDA, 3.69 ± 0.3 pmol per mg), vitamin E was depleted (α-tocopherol, 2.90 ± 0.6 pmol per mg, p < 0.001; γ-tocopherol, 0.5 ± 0.1 pmol per mg, p < 0.001) and MDA levels were increased (4.5 ± 0.2; p < 0.01). This report demonstrates the unique susceptibility of the SC to oxidative damage upon exposure to O3.

Original languageEnglish (US)
Pages (from-to)753-757
Number of pages5
JournalJournal of Investigative Dermatology
Volume108
Issue number5
StatePublished - 1997

Fingerprint

Tocopherols
Ozone
Malondialdehyde
Vitamin E
Cornea
Lipid Peroxidation
Lipids
Tapes
Skin
Smog
Oxidative stress
Hairless Mouse
Health
Tissue
Permeability
Oxidative Stress
High Pressure Liquid Chromatography

Keywords

  • anti-oxidants
  • oxidative stress
  • skin
  • tocopherol

ASJC Scopus subject areas

  • Dermatology

Cite this

Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum. / Thiele, Jens J.; Traber, Maret G.; Polefka, Thomas G.; Cross, Carroll E; Packer, Lester.

In: Journal of Investigative Dermatology, Vol. 108, No. 5, 1997, p. 753-757.

Research output: Contribution to journalArticle

Thiele, Jens J. ; Traber, Maret G. ; Polefka, Thomas G. ; Cross, Carroll E ; Packer, Lester. / Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum. In: Journal of Investigative Dermatology. 1997 ; Vol. 108, No. 5. pp. 753-757.
@article{2a31e583110b48248b9750884c3cf093,
title = "Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum",
abstract = "The presence of ozone (O3) in photochemical smog is an important health concern. We hypothesized that the stratum corneum (SC), as the outermost skin layer and the permeability barrier of the skin, represents a sensitive target for O3-induced oxidative stress. To test this hypothesis, SKH-1 hairless mice were anesthetized and exposed for 2 h to O3 by using two strategies: (i) single exposures to 0 (n = 12), 1 (n = 4), 5 (n = 4), and 10 (n = 4) ppm; and (ii) repeated daily exposures to 0 ppm (controls; n = 4) and 1 ppm (n = 4) for six consecutive days. New techniques based on the removal of SC by tape stripping were used to analyze the biologic effects of O3 with respect to vitamin E depletion and lipid peroxidation. SC tissue was extracted from the tape and immediately analyzed by HPLC for vitamin E and malondialdehyde (MDA) concentrations. After in vivo exposure to increasing O3 doses, vitamin E was depleted and MDA formation was increased, both in a dose-dependent manner. Remarkably, repeated low-level O3 exposures resulted in cumulative oxidative effects in the SC: As compared with O3 exposures of 0 ppm (α-tocopherol, 8.95 ± 1.3 pmol per mg; γ-tocopherol, 3.00 ± 0.3 pmol per mg; MDA, 3.69 ± 0.3 pmol per mg), vitamin E was depleted (α-tocopherol, 2.90 ± 0.6 pmol per mg, p < 0.001; γ-tocopherol, 0.5 ± 0.1 pmol per mg, p < 0.001) and MDA levels were increased (4.5 ± 0.2; p < 0.01). This report demonstrates the unique susceptibility of the SC to oxidative damage upon exposure to O3.",
keywords = "anti-oxidants, oxidative stress, skin, tocopherol",
author = "Thiele, {Jens J.} and Traber, {Maret G.} and Polefka, {Thomas G.} and Cross, {Carroll E} and Lester Packer",
year = "1997",
language = "English (US)",
volume = "108",
pages = "753--757",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Ozone-exposure depletes vitamin E and induces lipid peroxidation in murine stratum corneum

AU - Thiele, Jens J.

AU - Traber, Maret G.

AU - Polefka, Thomas G.

AU - Cross, Carroll E

AU - Packer, Lester

PY - 1997

Y1 - 1997

N2 - The presence of ozone (O3) in photochemical smog is an important health concern. We hypothesized that the stratum corneum (SC), as the outermost skin layer and the permeability barrier of the skin, represents a sensitive target for O3-induced oxidative stress. To test this hypothesis, SKH-1 hairless mice were anesthetized and exposed for 2 h to O3 by using two strategies: (i) single exposures to 0 (n = 12), 1 (n = 4), 5 (n = 4), and 10 (n = 4) ppm; and (ii) repeated daily exposures to 0 ppm (controls; n = 4) and 1 ppm (n = 4) for six consecutive days. New techniques based on the removal of SC by tape stripping were used to analyze the biologic effects of O3 with respect to vitamin E depletion and lipid peroxidation. SC tissue was extracted from the tape and immediately analyzed by HPLC for vitamin E and malondialdehyde (MDA) concentrations. After in vivo exposure to increasing O3 doses, vitamin E was depleted and MDA formation was increased, both in a dose-dependent manner. Remarkably, repeated low-level O3 exposures resulted in cumulative oxidative effects in the SC: As compared with O3 exposures of 0 ppm (α-tocopherol, 8.95 ± 1.3 pmol per mg; γ-tocopherol, 3.00 ± 0.3 pmol per mg; MDA, 3.69 ± 0.3 pmol per mg), vitamin E was depleted (α-tocopherol, 2.90 ± 0.6 pmol per mg, p < 0.001; γ-tocopherol, 0.5 ± 0.1 pmol per mg, p < 0.001) and MDA levels were increased (4.5 ± 0.2; p < 0.01). This report demonstrates the unique susceptibility of the SC to oxidative damage upon exposure to O3.

AB - The presence of ozone (O3) in photochemical smog is an important health concern. We hypothesized that the stratum corneum (SC), as the outermost skin layer and the permeability barrier of the skin, represents a sensitive target for O3-induced oxidative stress. To test this hypothesis, SKH-1 hairless mice were anesthetized and exposed for 2 h to O3 by using two strategies: (i) single exposures to 0 (n = 12), 1 (n = 4), 5 (n = 4), and 10 (n = 4) ppm; and (ii) repeated daily exposures to 0 ppm (controls; n = 4) and 1 ppm (n = 4) for six consecutive days. New techniques based on the removal of SC by tape stripping were used to analyze the biologic effects of O3 with respect to vitamin E depletion and lipid peroxidation. SC tissue was extracted from the tape and immediately analyzed by HPLC for vitamin E and malondialdehyde (MDA) concentrations. After in vivo exposure to increasing O3 doses, vitamin E was depleted and MDA formation was increased, both in a dose-dependent manner. Remarkably, repeated low-level O3 exposures resulted in cumulative oxidative effects in the SC: As compared with O3 exposures of 0 ppm (α-tocopherol, 8.95 ± 1.3 pmol per mg; γ-tocopherol, 3.00 ± 0.3 pmol per mg; MDA, 3.69 ± 0.3 pmol per mg), vitamin E was depleted (α-tocopherol, 2.90 ± 0.6 pmol per mg, p < 0.001; γ-tocopherol, 0.5 ± 0.1 pmol per mg, p < 0.001) and MDA levels were increased (4.5 ± 0.2; p < 0.01). This report demonstrates the unique susceptibility of the SC to oxidative damage upon exposure to O3.

KW - anti-oxidants

KW - oxidative stress

KW - skin

KW - tocopherol

UR - http://www.scopus.com/inward/record.url?scp=0030983673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030983673&partnerID=8YFLogxK

M3 - Article

VL - 108

SP - 753

EP - 757

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 5

ER -