Ozone exposure activates oxidative stress responses in murine skin

Giuseppe Valacchi, Albert Van der Vliet, Bettina C. Schock, Tatsuya Okamoto, Ute Obermuller-Jevic, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


Ozone (O3) is among the most reactive environmental oxidant to which skin is exposed. O3 exposure has previously been shown to induce antioxidant depletion as well as lipid and protein oxidation in the outermost skin layer, the stratum corneum (SC), but little is known regarding the potential effects of O3 on the skin epidermis and dermis. To evaluate such skin responses to O3, SKH-1 hairless mice were exposed for 2 h to 8.0 ppm O3 or to ambient air. O3 exposure caused a significant increase in skin carbonyls (28%) compared to the skin of air exposed control animals. An evident increase in 4-hydroxynonenal-protein adducts was detected after O3 exposure. O3 exposure caused a rapid up-regulation of HSP27 (20-fold), and more delayed induction of HSP70 (2.8-fold) and heme oxygenase-1 (5-fold). O3 exposure also led to the induction of nitric oxide synthase (iNOS) 6-12 h following O3 exposure. We conclude that skin exposure to high levels of O3 not only affects antioxidant levels and oxidation markers in the SC, but also induces stress responses in the active layers of the skin, most likely by indirect mechanisms, since it is unlikely that O3 itself penetrates the protective SC layers.

Original languageEnglish (US)
Pages (from-to)163-170
Number of pages8
Issue number1-2
StatePublished - Sep 30 2002


  • Heat stress proteins
  • Heme oxygenase
  • Hydroxynonenal
  • Keratinocytes
  • Nitric oxide

ASJC Scopus subject areas

  • Toxicology


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