Abstract
To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRP-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-170 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 401 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jan 20 1997 |
| Externally published | Yes |
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Keywords
- Antioxidant
- Cutaneous lipid
- Epidermis
- Hairless mice
- Malondialdehyde
- Vitamin E
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Ozone depletes tocopherols and tocotrienols topically applied to murine skin. / Thiele, Jens J.; Traber, Maret G.; Podda, Maurizio; Tsang, Kenneth; Cross, Carroll E; Packer, Lester.
In: FEBS Letters, Vol. 401, No. 2-3, 20.01.1997, p. 167-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ozone depletes tocopherols and tocotrienols topically applied to murine skin
AU - Thiele, Jens J.
AU - Traber, Maret G.
AU - Podda, Maurizio
AU - Tsang, Kenneth
AU - Cross, Carroll E
AU - Packer, Lester
PY - 1997/1/20
Y1 - 1997/1/20
N2 - To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRP-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.
AB - To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRP-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.
KW - Antioxidant
KW - Cutaneous lipid
KW - Epidermis
KW - Hairless mice
KW - Malondialdehyde
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=0031036035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031036035&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(96)01463-9
DO - 10.1016/S0014-5793(96)01463-9
M3 - Article
C2 - 9013880
AN - SCOPUS:0031036035
VL - 401
SP - 167
EP - 170
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -