Ozone depletes tocopherols and tocotrienols topically applied to murine skin

Jens J. Thiele, Maret G. Traber, Maurizio Podda, Kenneth Tsang, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRP-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalFEBS Letters
Volume401
Issue number2-3
DOIs
StatePublished - Jan 20 1997
Externally publishedYes

Fingerprint

Tocotrienols
Tocopherols
Ozone
Vitamin E
Skin
Malondialdehyde
Hairless Mouse
Demonstrations
Lipids

Keywords

  • Antioxidant
  • Cutaneous lipid
  • Epidermis
  • Hairless mice
  • Malondialdehyde
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ozone depletes tocopherols and tocotrienols topically applied to murine skin. / Thiele, Jens J.; Traber, Maret G.; Podda, Maurizio; Tsang, Kenneth; Cross, Carroll E; Packer, Lester.

In: FEBS Letters, Vol. 401, No. 2-3, 20.01.1997, p. 167-170.

Research output: Contribution to journalArticle

Thiele, JJ, Traber, MG, Podda, M, Tsang, K, Cross, CE & Packer, L 1997, 'Ozone depletes tocopherols and tocotrienols topically applied to murine skin', FEBS Letters, vol. 401, no. 2-3, pp. 167-170. https://doi.org/10.1016/S0014-5793(96)01463-9
Thiele, Jens J. ; Traber, Maret G. ; Podda, Maurizio ; Tsang, Kenneth ; Cross, Carroll E ; Packer, Lester. / Ozone depletes tocopherols and tocotrienols topically applied to murine skin. In: FEBS Letters. 1997 ; Vol. 401, No. 2-3. pp. 167-170.
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