Ozone depletes tocopherols and tocotrienols topically applied to murine skin

Jens J. Thiele, Maret G. Traber, Maurizio Podda, Kenneth Tsang, Carroll E. Cross, Lester Packer

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRP-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalFEBS Letters
Issue number2-3
StatePublished - Jan 20 1997
Externally publishedYes


  • Antioxidant
  • Cutaneous lipid
  • Epidermis
  • Hairless mice
  • Malondialdehyde
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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