Abstract
To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRP-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.
Original language | English (US) |
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Pages (from-to) | 167-170 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 401 |
Issue number | 2-3 |
DOIs | |
State | Published - Jan 20 1997 |
Externally published | Yes |
Keywords
- Antioxidant
- Cutaneous lipid
- Epidermis
- Hairless mice
- Malondialdehyde
- Vitamin E
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology