Oxygen radical production by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils

Lisa A Tell, Mahendra B. Kabbur, Wayne L. Smith, Laurie Gage, James S Cullor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Oxidative metabolic burst activity by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils was indirectly evaluated by measuring the oxidation of nonfluorescent intracellular 2′,7′-dichlorofluorescin (DCFH) to fluorescent 2′,7′-dichlorofluorescein (DCF) with flow cytometry. The results were recorded as mean channel fluorescence intensity. Phorbol myristate acetate at 50 ng/ml was used to activate the cells. Elephant heterophils and bovine neutrophils exhibited similar abilities to oxidize intracellular DCFH to DCF, a fluorescent product. A wide range of mean channel fluorescence intensity (1,803-7,393) was observed among individual elephants, and the range of intensities was similar to that observed in bovine neutrophils, supporting the concept of functional heterogeneity of heterophils among elephants.

Original languageEnglish (US)
Pages (from-to)402-407
Number of pages6
JournalJournal of Zoo and Wildlife Medicine
Volume30
Issue number3
StatePublished - Sep 1999

Fingerprint

Elephas maximus
heterophils
elephant
neutrophils
cattle
reactive oxygen species
Reactive Oxygen Species
Elephantidae
Neutrophils
Holstein
oxygen
fluorescence
Fluorescence
flow cytometry
Respiratory Burst
Tetradecanoylphorbol Acetate
acetate
acetates
oxidation
Flow Cytometry

Keywords

  • Cattle
  • Elephant
  • Flow cytometry
  • Heterophils
  • Neutrophils
  • Oxidative product formation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • veterinary(all)

Cite this

Oxygen radical production by Asian elephant (Elephas maximus) heterophils and Holstein cattle (Bos taurus) neutrophils. / Tell, Lisa A; Kabbur, Mahendra B.; Smith, Wayne L.; Gage, Laurie; Cullor, James S.

In: Journal of Zoo and Wildlife Medicine, Vol. 30, No. 3, 09.1999, p. 402-407.

Research output: Contribution to journalArticle

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