Oxygen radical injury in the immature isolated rabbit heart

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We speculated that the increased vulnerability of the immature rabbit heart to global ischemia might be due to an increased susceptibility to free radical injury. To evaluate this, we exposed newborn (age 2.4 ± 0.3 days, n=20) (mean ± SEM), juvenile (2 to 3 weeks, mean 16.6 ± 0.5 days, n=20), and adult (5 to 7 months old, n=20) isolated, idovolumic, Krebs perfused rabbit hearts to oxygen radicals or cumene hydroperoxide. Control hearts showed no deterioration in left ventricular developed pressure over 60 min (newborns = 104 ± 11%, juveniles = 101 ± 7%, and adults = 113 ± 12% of baseline, n=5 for each age group). After only 30 min of oxygen radical exposure, the newborn group developed pressure decreased to 49 ± 6% of the baseline value, while juveniles and adults were functioning at 70 ± 10% and 83 ± 6% of baseline, respectively (n=10 for each age group) (P<0.05, newborn different from adult group). In contrast to the oxygen radical protocol, the hearts exposed to cumene hydroperoxide showed no significant difference between the age groups in deterioration of left ventricular function. There was no significant difference between the age groups in ATP content or thiobarbituric reactive substances following the oxygen radical exposure. We conclude that the newborn rabbit heart is significantly more vulnerable than the adult heart to the toxic effects of oxygen radicais. This may account, in part, for age related differences in response to global ischemia and reperfusion.

Original languageEnglish (US)
Pages (from-to)243-249
Number of pages7
JournalJournal of Developmental Physiology
Volume16
Issue number4
StatePublished - 1991

Fingerprint

Reactive Oxygen Species
Rabbits
Wounds and Injuries
Age Groups
Newborn Infant
Ischemia
Poisons
Ventricular Pressure
Left Ventricular Function
Reperfusion
Free Radicals
Adenosine Triphosphate
Oxygen
Pressure
cumene hydroperoxide

ASJC Scopus subject areas

  • Developmental Biology
  • Physiology

Cite this

Oxygen radical injury in the immature isolated rabbit heart. / Parrish, Mark D.

In: Journal of Developmental Physiology, Vol. 16, No. 4, 1991, p. 243-249.

Research output: Contribution to journalArticle

@article{a242c40d0a684b1ab9d0abf7f1f8e7e7,
title = "Oxygen radical injury in the immature isolated rabbit heart",
abstract = "We speculated that the increased vulnerability of the immature rabbit heart to global ischemia might be due to an increased susceptibility to free radical injury. To evaluate this, we exposed newborn (age 2.4 ± 0.3 days, n=20) (mean ± SEM), juvenile (2 to 3 weeks, mean 16.6 ± 0.5 days, n=20), and adult (5 to 7 months old, n=20) isolated, idovolumic, Krebs perfused rabbit hearts to oxygen radicals or cumene hydroperoxide. Control hearts showed no deterioration in left ventricular developed pressure over 60 min (newborns = 104 ± 11{\%}, juveniles = 101 ± 7{\%}, and adults = 113 ± 12{\%} of baseline, n=5 for each age group). After only 30 min of oxygen radical exposure, the newborn group developed pressure decreased to 49 ± 6{\%} of the baseline value, while juveniles and adults were functioning at 70 ± 10{\%} and 83 ± 6{\%} of baseline, respectively (n=10 for each age group) (P<0.05, newborn different from adult group). In contrast to the oxygen radical protocol, the hearts exposed to cumene hydroperoxide showed no significant difference between the age groups in deterioration of left ventricular function. There was no significant difference between the age groups in ATP content or thiobarbituric reactive substances following the oxygen radical exposure. We conclude that the newborn rabbit heart is significantly more vulnerable than the adult heart to the toxic effects of oxygen radicais. This may account, in part, for age related differences in response to global ischemia and reperfusion.",
author = "Parrish, {Mark D}",
year = "1991",
language = "English (US)",
volume = "16",
pages = "243--249",
journal = "Journal of Developmental Physiology",
issn = "0141-9846",
number = "4",

}

TY - JOUR

T1 - Oxygen radical injury in the immature isolated rabbit heart

AU - Parrish, Mark D

PY - 1991

Y1 - 1991

N2 - We speculated that the increased vulnerability of the immature rabbit heart to global ischemia might be due to an increased susceptibility to free radical injury. To evaluate this, we exposed newborn (age 2.4 ± 0.3 days, n=20) (mean ± SEM), juvenile (2 to 3 weeks, mean 16.6 ± 0.5 days, n=20), and adult (5 to 7 months old, n=20) isolated, idovolumic, Krebs perfused rabbit hearts to oxygen radicals or cumene hydroperoxide. Control hearts showed no deterioration in left ventricular developed pressure over 60 min (newborns = 104 ± 11%, juveniles = 101 ± 7%, and adults = 113 ± 12% of baseline, n=5 for each age group). After only 30 min of oxygen radical exposure, the newborn group developed pressure decreased to 49 ± 6% of the baseline value, while juveniles and adults were functioning at 70 ± 10% and 83 ± 6% of baseline, respectively (n=10 for each age group) (P<0.05, newborn different from adult group). In contrast to the oxygen radical protocol, the hearts exposed to cumene hydroperoxide showed no significant difference between the age groups in deterioration of left ventricular function. There was no significant difference between the age groups in ATP content or thiobarbituric reactive substances following the oxygen radical exposure. We conclude that the newborn rabbit heart is significantly more vulnerable than the adult heart to the toxic effects of oxygen radicais. This may account, in part, for age related differences in response to global ischemia and reperfusion.

AB - We speculated that the increased vulnerability of the immature rabbit heart to global ischemia might be due to an increased susceptibility to free radical injury. To evaluate this, we exposed newborn (age 2.4 ± 0.3 days, n=20) (mean ± SEM), juvenile (2 to 3 weeks, mean 16.6 ± 0.5 days, n=20), and adult (5 to 7 months old, n=20) isolated, idovolumic, Krebs perfused rabbit hearts to oxygen radicals or cumene hydroperoxide. Control hearts showed no deterioration in left ventricular developed pressure over 60 min (newborns = 104 ± 11%, juveniles = 101 ± 7%, and adults = 113 ± 12% of baseline, n=5 for each age group). After only 30 min of oxygen radical exposure, the newborn group developed pressure decreased to 49 ± 6% of the baseline value, while juveniles and adults were functioning at 70 ± 10% and 83 ± 6% of baseline, respectively (n=10 for each age group) (P<0.05, newborn different from adult group). In contrast to the oxygen radical protocol, the hearts exposed to cumene hydroperoxide showed no significant difference between the age groups in deterioration of left ventricular function. There was no significant difference between the age groups in ATP content or thiobarbituric reactive substances following the oxygen radical exposure. We conclude that the newborn rabbit heart is significantly more vulnerable than the adult heart to the toxic effects of oxygen radicais. This may account, in part, for age related differences in response to global ischemia and reperfusion.

UR - http://www.scopus.com/inward/record.url?scp=0026387884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026387884&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 243

EP - 249

JO - Journal of Developmental Physiology

JF - Journal of Developmental Physiology

SN - 0141-9846

IS - 4

ER -