Oximetry with the NMR signals of hemoglobin Val E11 and Tyr C7

Hongtao Xie, Ulrike Kreutzer, Thomas Jue

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The NMR visibility of the signals from erythrocyte hemoglobin (Hb) presents an opportunity to assess the vascular PO2 (partial pressure of oxygen) in vivo to gather insight into the regulation of O2 transport, especially in contracting muscle tissue. Some concerns, however, have arisen about the validity of using the Val E11 signal as an indicator of PO2, since its intensity depends on tertiary structural changes, in contrast to the quaternary structure changes associated with relaxed (R) and tense (T) transition during O2 binding. We have examined the Val E11 and Tyr C7 signal intensity as a function of Hb saturation by developing an oximetry system, which permits the comparative analysis of the NMR and spectrophotometric measurements. The spectrophotometric assay defines the Hb saturation level at a given PO2 and yields standard oxygen-binding curves. Under defined PO2 and Hb saturation values, the NMR measurements have determined that the Val E11 signal, as well as the Tyr C7 signal, tracks closely Hb saturation and can therefore serve as a vascular oxygen biomarker.

Original languageEnglish (US)
Pages (from-to)325-333
Number of pages9
JournalEuropean Journal of Applied Physiology
Volume107
Issue number3
DOIs
StatePublished - 2009

Fingerprint

Oximetry
Hemoglobins
Oxygen
Blood Vessels
Partial Pressure
Erythrocytes
Biomarkers
Muscles

Keywords

  • Bioenergetics
  • Muscle
  • Myoglobin
  • Oxygen
  • Oxygen transport

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Orthopedics and Sports Medicine
  • Physiology (medical)

Cite this

Oximetry with the NMR signals of hemoglobin Val E11 and Tyr C7. / Xie, Hongtao; Kreutzer, Ulrike; Jue, Thomas.

In: European Journal of Applied Physiology, Vol. 107, No. 3, 2009, p. 325-333.

Research output: Contribution to journalArticle

Xie, Hongtao ; Kreutzer, Ulrike ; Jue, Thomas. / Oximetry with the NMR signals of hemoglobin Val E11 and Tyr C7. In: European Journal of Applied Physiology. 2009 ; Vol. 107, No. 3. pp. 325-333.
@article{2932cad21f7d4165969c510d8e2428ba,
title = "Oximetry with the NMR signals of hemoglobin Val E11 and Tyr C7",
abstract = "The NMR visibility of the signals from erythrocyte hemoglobin (Hb) presents an opportunity to assess the vascular PO2 (partial pressure of oxygen) in vivo to gather insight into the regulation of O2 transport, especially in contracting muscle tissue. Some concerns, however, have arisen about the validity of using the Val E11 signal as an indicator of PO2, since its intensity depends on tertiary structural changes, in contrast to the quaternary structure changes associated with relaxed (R) and tense (T) transition during O2 binding. We have examined the Val E11 and Tyr C7 signal intensity as a function of Hb saturation by developing an oximetry system, which permits the comparative analysis of the NMR and spectrophotometric measurements. The spectrophotometric assay defines the Hb saturation level at a given PO2 and yields standard oxygen-binding curves. Under defined PO2 and Hb saturation values, the NMR measurements have determined that the Val E11 signal, as well as the Tyr C7 signal, tracks closely Hb saturation and can therefore serve as a vascular oxygen biomarker.",
keywords = "Bioenergetics, Muscle, Myoglobin, Oxygen, Oxygen transport",
author = "Hongtao Xie and Ulrike Kreutzer and Thomas Jue",
year = "2009",
doi = "10.1007/s00421-009-1125-3",
language = "English (US)",
volume = "107",
pages = "325--333",
journal = "European Journal of Applied Physiology",
issn = "1439-6319",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Oximetry with the NMR signals of hemoglobin Val E11 and Tyr C7

AU - Xie, Hongtao

AU - Kreutzer, Ulrike

AU - Jue, Thomas

PY - 2009

Y1 - 2009

N2 - The NMR visibility of the signals from erythrocyte hemoglobin (Hb) presents an opportunity to assess the vascular PO2 (partial pressure of oxygen) in vivo to gather insight into the regulation of O2 transport, especially in contracting muscle tissue. Some concerns, however, have arisen about the validity of using the Val E11 signal as an indicator of PO2, since its intensity depends on tertiary structural changes, in contrast to the quaternary structure changes associated with relaxed (R) and tense (T) transition during O2 binding. We have examined the Val E11 and Tyr C7 signal intensity as a function of Hb saturation by developing an oximetry system, which permits the comparative analysis of the NMR and spectrophotometric measurements. The spectrophotometric assay defines the Hb saturation level at a given PO2 and yields standard oxygen-binding curves. Under defined PO2 and Hb saturation values, the NMR measurements have determined that the Val E11 signal, as well as the Tyr C7 signal, tracks closely Hb saturation and can therefore serve as a vascular oxygen biomarker.

AB - The NMR visibility of the signals from erythrocyte hemoglobin (Hb) presents an opportunity to assess the vascular PO2 (partial pressure of oxygen) in vivo to gather insight into the regulation of O2 transport, especially in contracting muscle tissue. Some concerns, however, have arisen about the validity of using the Val E11 signal as an indicator of PO2, since its intensity depends on tertiary structural changes, in contrast to the quaternary structure changes associated with relaxed (R) and tense (T) transition during O2 binding. We have examined the Val E11 and Tyr C7 signal intensity as a function of Hb saturation by developing an oximetry system, which permits the comparative analysis of the NMR and spectrophotometric measurements. The spectrophotometric assay defines the Hb saturation level at a given PO2 and yields standard oxygen-binding curves. Under defined PO2 and Hb saturation values, the NMR measurements have determined that the Val E11 signal, as well as the Tyr C7 signal, tracks closely Hb saturation and can therefore serve as a vascular oxygen biomarker.

KW - Bioenergetics

KW - Muscle

KW - Myoglobin

KW - Oxygen

KW - Oxygen transport

UR - http://www.scopus.com/inward/record.url?scp=70349742374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349742374&partnerID=8YFLogxK

U2 - 10.1007/s00421-009-1125-3

DO - 10.1007/s00421-009-1125-3

M3 - Article

C2 - 19621237

AN - SCOPUS:70349742374

VL - 107

SP - 325

EP - 333

JO - European Journal of Applied Physiology

JF - European Journal of Applied Physiology

SN - 1439-6319

IS - 3

ER -