Oxidative stress decreases microtubule growth and stability in ventricular myocytes

Benjamin M L Drum, Can Yuan, Lei Li, Qinghang Liu, Linda Wordeman, Luis Fernando Santana

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Microtubules (MTs) have many roles in ventricular myocytes, including structural stability, morphological integrity, and protein trafficking. However, despite their functional importance, dynamic MTs had never been visualized in living adult myocytes. Using adeno-associated viral vectors expressing the MT-associated protein plus end binding protein 3 (EB3) tagged with EGFP, we were able to perform live imaging and thus capture and quantify MT dynamics in ventricular myocytes in real time under physiological conditions. Super-resolution nanoscopy revealed that EB1 associated in puncta along the length of MTs in ventricular myocytes. The vast (~80%) majority of MTs grew perpendicular to T-tubules at a rate of 0.06 μm * s-1 and growth was preferentially (82%) confined to a single sarcomere. Microtubule catastrophe rate was lower near the Z-line than M-line. Hydrogen peroxide increased the rate of catastrophe of MTs ~7-fold, suggesting that oxidative stress destabilizes these structures in ventricular myocytes. We also quantified MT dynamics after myocardial infarction (MI), a pathological condition associated with increased production of reactive oxygen species (ROS). Our data indicate that the catastrophe rate of MTs increases following MI. This contributed to decreased transient outward K+ currents by decreasing the surface expression of Kv4.2 and Kv4.3 channels after MI. On the basis of these data, we conclude that, under physiological conditions, MT growth is directionally biased and that increased ROS production during MI disrupts MT dynamics, decreasing K+ channel trafficking.

Original languageEnglish (US)
Pages (from-to)32-43
Number of pages12
JournalJournal of Molecular and Cellular Cardiology
Volume93
DOIs
StatePublished - Apr 1 2016

Fingerprint

Microtubules
Muscle Cells
Oxidative Stress
Growth
Myocardial Infarction
Reactive Oxygen Species
Sarcomeres
Microtubule-Associated Proteins
Protein Transport
Hydrogen Peroxide
Carrier Proteins

Keywords

  • Cardiomyocytes
  • Live imaging
  • Microtubule dynamics
  • Myocardial infarction
  • Oxidative stress
  • Transient outward current

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Oxidative stress decreases microtubule growth and stability in ventricular myocytes. / Drum, Benjamin M L; Yuan, Can; Li, Lei; Liu, Qinghang; Wordeman, Linda; Santana, Luis Fernando.

In: Journal of Molecular and Cellular Cardiology, Vol. 93, 01.04.2016, p. 32-43.

Research output: Contribution to journalArticle

Drum, Benjamin M L ; Yuan, Can ; Li, Lei ; Liu, Qinghang ; Wordeman, Linda ; Santana, Luis Fernando. / Oxidative stress decreases microtubule growth and stability in ventricular myocytes. In: Journal of Molecular and Cellular Cardiology. 2016 ; Vol. 93. pp. 32-43.
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