Abstract
When human respiratory tract epithelial cells were exposed to 100 μM H2O2, there was rapid induction of DNA strand breakage and chemical modifications to all 4 DNA bases suggestive of attack by OH(̇). The major products were FAPy-adenine, FAPy-guanine, and 8-OH-guanine. Some of the base modifications were removed very quickly from the DNA (e.g., 8-OH-guanine), whereas others persisted for longer (e.g., thymine glycol), probably due to differential activity of different repair enzymes. By contrast, strand breaks continued to increase over the rime course of the experiment, perhaps because strand breakage is also implicated in the repair process. One should therefore be cautious in using strand breakage as a sole measure of oxidative DNA damage, and when drawing conclusions about the pattern and biological significance of oxidative DNA damage in cells the relative persistence of different lesions must be considered.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17-22 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 224 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 5 1996 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Oxidative DNA damage in human respiratory tract epithelial cells. Time course in relation to DNA strand breakage. / Spencer, Jeremy P E; Jenner, Andrew; Aruoma, Okezie I.; Cross, Carroll E; Wu, Reen; Halliwell, Barry.
In: Biochemical and Biophysical Research Communications, Vol. 224, No. 1, 05.07.1996, p. 17-22.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Oxidative DNA damage in human respiratory tract epithelial cells. Time course in relation to DNA strand breakage
AU - Spencer, Jeremy P E
AU - Jenner, Andrew
AU - Aruoma, Okezie I.
AU - Cross, Carroll E
AU - Wu, Reen
AU - Halliwell, Barry
PY - 1996/7/5
Y1 - 1996/7/5
N2 - When human respiratory tract epithelial cells were exposed to 100 μM H2O2, there was rapid induction of DNA strand breakage and chemical modifications to all 4 DNA bases suggestive of attack by OH(̇). The major products were FAPy-adenine, FAPy-guanine, and 8-OH-guanine. Some of the base modifications were removed very quickly from the DNA (e.g., 8-OH-guanine), whereas others persisted for longer (e.g., thymine glycol), probably due to differential activity of different repair enzymes. By contrast, strand breaks continued to increase over the rime course of the experiment, perhaps because strand breakage is also implicated in the repair process. One should therefore be cautious in using strand breakage as a sole measure of oxidative DNA damage, and when drawing conclusions about the pattern and biological significance of oxidative DNA damage in cells the relative persistence of different lesions must be considered.
AB - When human respiratory tract epithelial cells were exposed to 100 μM H2O2, there was rapid induction of DNA strand breakage and chemical modifications to all 4 DNA bases suggestive of attack by OH(̇). The major products were FAPy-adenine, FAPy-guanine, and 8-OH-guanine. Some of the base modifications were removed very quickly from the DNA (e.g., 8-OH-guanine), whereas others persisted for longer (e.g., thymine glycol), probably due to differential activity of different repair enzymes. By contrast, strand breaks continued to increase over the rime course of the experiment, perhaps because strand breakage is also implicated in the repair process. One should therefore be cautious in using strand breakage as a sole measure of oxidative DNA damage, and when drawing conclusions about the pattern and biological significance of oxidative DNA damage in cells the relative persistence of different lesions must be considered.
UR - http://www.scopus.com/inward/record.url?scp=0030570517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030570517&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0977
DO - 10.1006/bbrc.1996.0977
M3 - Article
C2 - 8694807
AN - SCOPUS:0030570517
VL - 224
SP - 17
EP - 22
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -