Oxidative DNA damage in human respiratory tract epithelial cells. Time course in relation to DNA strand breakage

Jeremy P E Spencer, Andrew Jenner, Okezie I. Aruoma, Carroll E Cross, Reen Wu, Barry Halliwell

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

When human respiratory tract epithelial cells were exposed to 100 μM H2O2, there was rapid induction of DNA strand breakage and chemical modifications to all 4 DNA bases suggestive of attack by OH(̇). The major products were FAPy-adenine, FAPy-guanine, and 8-OH-guanine. Some of the base modifications were removed very quickly from the DNA (e.g., 8-OH-guanine), whereas others persisted for longer (e.g., thymine glycol), probably due to differential activity of different repair enzymes. By contrast, strand breaks continued to increase over the rime course of the experiment, perhaps because strand breakage is also implicated in the repair process. One should therefore be cautious in using strand breakage as a sole measure of oxidative DNA damage, and when drawing conclusions about the pattern and biological significance of oxidative DNA damage in cells the relative persistence of different lesions must be considered.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume224
Issue number1
DOIs
StatePublished - Jul 5 1996

Fingerprint

Respiratory System
DNA Damage
Epithelial Cells
Guanine
DNA
Adenine
Repair
Chemical modification
Enzymes
hydroxide ion
Experiments
2,6-diamino-4-hydroxy-5-formamidopyrimidine
4,6-diamino-5-N-formamidopyrimidine
thymine glycol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Oxidative DNA damage in human respiratory tract epithelial cells. Time course in relation to DNA strand breakage. / Spencer, Jeremy P E; Jenner, Andrew; Aruoma, Okezie I.; Cross, Carroll E; Wu, Reen; Halliwell, Barry.

In: Biochemical and Biophysical Research Communications, Vol. 224, No. 1, 05.07.1996, p. 17-22.

Research output: Contribution to journalArticle

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