Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation

Shida Yousefi, Satish K. Sharma, Darko Stojkov, Nina Germic, Salome Aeschlimann, Moyar Q. Ge, Cameron H. Flayer, Erik D. Larson, Imre G. Redai, Suhong Zhang, Cynthia J. Koziol-White, Katalin Karikó, Hans Uwe Simon, Angela Franciska Haczku

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The asthmatic airways are highly susceptible to inflammatory injury by air pollutants such as ozone (O3), characterized by enhanced activation of eosinophilic granulocytes and a failure of immune protective mechanisms. Eosinophil activation during asthma exacerbation contributes to the proinflammatory oxidative stress by high levels of nitric oxide (NO) production and extracellular DNA release. Surfactant protein-D (SP-D), an epithelial cell product of the airways, is a critical immune regulatory molecule with a multimeric structure susceptible to oxidative modifications. Using recombinant proteins and confocal imaging, we demonstrate here that SP-D directly bound to the membrane and inhibited extracellular DNA trap formation by human and murine eosinophils in a concentration and carbohydrate-dependent manner. Combined allergic airway sensitization and O3 exposure heightened eosinophilia and nos2 mRNA (iNOS) activation in the lung tissue and S-nitrosylation related de-oligomerisation of SP-D in the airways. In vitro reproduction of the iNOS action led to similar effects on SP-D. Importantly, S-nitrosylation abolished the ability of SP-D to block extracellular DNA trap formation. Thus, the homeostatic negative regulatory feedback between SP-D and eosinophils is destroyed by the NO-rich oxidative lung tissue environment in asthma exacerbations.

Original languageEnglish (US)
JournalJournal of Leukocyte Biology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Pulmonary Surfactant-Associated Protein D
Eosinophils
Nitric Oxide
Asthma
Lung
Air Pollutants
Ozone
Eosinophilia
Recombinant Proteins
Granulocytes
Reproduction
Extracellular Traps
Oxidative Stress
Epithelial Cells
Carbohydrates
Messenger RNA
Membranes
DNA
Wounds and Injuries

Keywords

  • Eosinophil extracellular traps (EETs)
  • Eosinophils
  • Surfactant pulmonary-associated protein D (SP-D)

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation. / Yousefi, Shida; Sharma, Satish K.; Stojkov, Darko; Germic, Nina; Aeschlimann, Salome; Ge, Moyar Q.; Flayer, Cameron H.; Larson, Erik D.; Redai, Imre G.; Zhang, Suhong; Koziol-White, Cynthia J.; Karikó, Katalin; Simon, Hans Uwe; Haczku, Angela Franciska.

In: Journal of Leukocyte Biology, 01.01.2018.

Research output: Contribution to journalArticle

Yousefi, S, Sharma, SK, Stojkov, D, Germic, N, Aeschlimann, S, Ge, MQ, Flayer, CH, Larson, ED, Redai, IG, Zhang, S, Koziol-White, CJ, Karikó, K, Simon, HU & Haczku, AF 2018, 'Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation', Journal of Leukocyte Biology. https://doi.org/10.1002/JLB.3AB1117-455R
Yousefi, Shida ; Sharma, Satish K. ; Stojkov, Darko ; Germic, Nina ; Aeschlimann, Salome ; Ge, Moyar Q. ; Flayer, Cameron H. ; Larson, Erik D. ; Redai, Imre G. ; Zhang, Suhong ; Koziol-White, Cynthia J. ; Karikó, Katalin ; Simon, Hans Uwe ; Haczku, Angela Franciska. / Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation. In: Journal of Leukocyte Biology. 2018.
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