Abstract
Ozone (O3) is a powerfully oxidizing pollutant gas. Its toxic effects to animals appear to be worsened by coexposures to acid-generating compounds such as oxides of nitrogen and sulfur. Ozone (16 ppm) oxidizes ascorbic acid and uric acid (two important antioxidants in lung llning fluids) at equal rates at pH 5.0 or pH 7.4. Loss of intrinsic fluorescence and formation of carbonyls in albumin exposed to O3 are similar at both pH values. However, albumin-SH groups are lost much faster on exposure to O3 at pH 7.4 than at acidic pH values. A similar slower rate of SH group disappearance at acidic pH is seen when cysteine or reduced glutathione are exposed to O3. We suggest that the ability of reduced glutathione, albumin, and other proteins containing SH groups to scavenge O3 in the respiratory tract is impaired at low pH and that this effect could contribute to the aggravation of O3 toxicity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 497-505 |
| Number of pages | 9 |
| Journal | The Journal of Laboratory and Clinical Medicine |
| Volume | 122 |
| Issue number | 5 |
| State | Published - 1993 |
Fingerprint
ASJC Scopus subject areas
- Medicine(all)
- Pathology and Forensic Medicine
Cite this
Oxidation of biologic molecules by ozone : The effect of pH. / O'Neill, Charles A.; van der Vliet, Albert; Hu, Miao Lin; Kaur, Harparkash; Cross, Carroll E; Louie, Samuel; Halliwell, Barry.
In: The Journal of Laboratory and Clinical Medicine, Vol. 122, No. 5, 1993, p. 497-505.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidation of biologic molecules by ozone
T2 - The effect of pH
AU - O'Neill, Charles A.
AU - van der Vliet, Albert
AU - Hu, Miao Lin
AU - Kaur, Harparkash
AU - Cross, Carroll E
AU - Louie, Samuel
AU - Halliwell, Barry
PY - 1993
Y1 - 1993
N2 - Ozone (O3) is a powerfully oxidizing pollutant gas. Its toxic effects to animals appear to be worsened by coexposures to acid-generating compounds such as oxides of nitrogen and sulfur. Ozone (16 ppm) oxidizes ascorbic acid and uric acid (two important antioxidants in lung llning fluids) at equal rates at pH 5.0 or pH 7.4. Loss of intrinsic fluorescence and formation of carbonyls in albumin exposed to O3 are similar at both pH values. However, albumin-SH groups are lost much faster on exposure to O3 at pH 7.4 than at acidic pH values. A similar slower rate of SH group disappearance at acidic pH is seen when cysteine or reduced glutathione are exposed to O3. We suggest that the ability of reduced glutathione, albumin, and other proteins containing SH groups to scavenge O3 in the respiratory tract is impaired at low pH and that this effect could contribute to the aggravation of O3 toxicity.
AB - Ozone (O3) is a powerfully oxidizing pollutant gas. Its toxic effects to animals appear to be worsened by coexposures to acid-generating compounds such as oxides of nitrogen and sulfur. Ozone (16 ppm) oxidizes ascorbic acid and uric acid (two important antioxidants in lung llning fluids) at equal rates at pH 5.0 or pH 7.4. Loss of intrinsic fluorescence and formation of carbonyls in albumin exposed to O3 are similar at both pH values. However, albumin-SH groups are lost much faster on exposure to O3 at pH 7.4 than at acidic pH values. A similar slower rate of SH group disappearance at acidic pH is seen when cysteine or reduced glutathione are exposed to O3. We suggest that the ability of reduced glutathione, albumin, and other proteins containing SH groups to scavenge O3 in the respiratory tract is impaired at low pH and that this effect could contribute to the aggravation of O3 toxicity.
UR - http://www.scopus.com/inward/record.url?scp=0027383768&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027383768&partnerID=8YFLogxK
M3 - Article
VL - 122
SP - 497
EP - 505
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 5
ER -