Oxidation of biologic molecules by ozone

The effect of pH

Charles A. O'Neill, Albert van der Vliet, Miao Lin Hu, Harparkash Kaur, Carroll E Cross, Samuel Louie, Barry Halliwell

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Ozone (O3) is a powerfully oxidizing pollutant gas. Its toxic effects to animals appear to be worsened by coexposures to acid-generating compounds such as oxides of nitrogen and sulfur. Ozone (16 ppm) oxidizes ascorbic acid and uric acid (two important antioxidants in lung llning fluids) at equal rates at pH 5.0 or pH 7.4. Loss of intrinsic fluorescence and formation of carbonyls in albumin exposed to O3 are similar at both pH values. However, albumin-SH groups are lost much faster on exposure to O3 at pH 7.4 than at acidic pH values. A similar slower rate of SH group disappearance at acidic pH is seen when cysteine or reduced glutathione are exposed to O3. We suggest that the ability of reduced glutathione, albumin, and other proteins containing SH groups to scavenge O3 in the respiratory tract is impaired at low pH and that this effect could contribute to the aggravation of O3 toxicity.

Original languageEnglish (US)
Pages (from-to)497-505
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume122
Issue number5
StatePublished - 1993

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Ozone
Albumins
Oxidation
Molecules
Glutathione
Sulfur Oxides
Poisons
Uric Acid
Ascorbic Acid
Cysteine
Toxicity
Animals
Antioxidants
Gases
Fluorescence
Nitrogen
Acids
Fluids
Respiratory System
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

O'Neill, C. A., van der Vliet, A., Hu, M. L., Kaur, H., Cross, C. E., Louie, S., & Halliwell, B. (1993). Oxidation of biologic molecules by ozone: The effect of pH. The Journal of Laboratory and Clinical Medicine, 122(5), 497-505.

Oxidation of biologic molecules by ozone : The effect of pH. / O'Neill, Charles A.; van der Vliet, Albert; Hu, Miao Lin; Kaur, Harparkash; Cross, Carroll E; Louie, Samuel; Halliwell, Barry.

In: The Journal of Laboratory and Clinical Medicine, Vol. 122, No. 5, 1993, p. 497-505.

Research output: Contribution to journalArticle

O'Neill, CA, van der Vliet, A, Hu, ML, Kaur, H, Cross, CE, Louie, S & Halliwell, B 1993, 'Oxidation of biologic molecules by ozone: The effect of pH', The Journal of Laboratory and Clinical Medicine, vol. 122, no. 5, pp. 497-505.
O'Neill, Charles A. ; van der Vliet, Albert ; Hu, Miao Lin ; Kaur, Harparkash ; Cross, Carroll E ; Louie, Samuel ; Halliwell, Barry. / Oxidation of biologic molecules by ozone : The effect of pH. In: The Journal of Laboratory and Clinical Medicine. 1993 ; Vol. 122, No. 5. pp. 497-505.
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