Oxidant stress inhibits the endogenous production of lipoxygenease metabolites in rat lungs and fish gills

J. Bruce German, Miao Lin Hu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Hydroperoxides are potent initiators of lipid peroxidation in vivo. Acyl hydroperoxides may also regulate various aspects of lipid metabolism. In this study we investigated the regulation of the endogenous 12 lipoxygenase in trout gill and rat lung, a prominant acyl hydroperoxide catalyst in these tissues. Initial experiments revealed that the enzyme from trout gill was activated by hydroperoxides at low levels and inactivated by the same hydroperoxides at high levels. Homogenization of these tissues resulted in the production of a predominant metabolite class from released endogenous polyunsaturated fatty acids, the 12 lipoxygenase products. In rat lung, arachidonic acid was the major polyunsaturated fatty acid released and 12 (S) HETE was the major metabolite. In trout gill 20:4, 20:5 n3, and 22:6 n3 were released and the 12(S), 12, and 14 hydroxy derivatives the corresponding metabolites. Computer simulations of the sensitivity of these enzymes to hydroperoxides predicted that exogenous oxidant stress would reduce significantly the production of HETEs. Tertiary butyl hydroperoxide was added to tissue homogenates and resulted in elimination of >95% of the lipoxygenase activity. These results suggest that the lipoxygenase enzyme in lung and gill tissue is a major potential source for acyl hydroperoxides in vivo, but is also very sensitive to oxidant including the acyl hydroperoxides themselves. This enzyme could thus be an important focus for oxidant injury in lungs.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalFree Radical Biology and Medicine
Volume8
Issue number5
DOIs
StatePublished - 1990

Fingerprint

Metabolites
Oxidants
Fish
Hydrogen Peroxide
Rats
Fishes
Lung
Trout
Arachidonate 12-Lipoxygenase
Tissue
Lipoxygenase
Enzymes
Unsaturated Fatty Acids
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Hydroxyeicosatetraenoic Acids
Lung Injury
Lipid Metabolism
Arachidonic Acid
Computer Simulation
Lipid Peroxidation

Keywords

  • 12-Lipoxygenase, 12 (S) Hydroxyeicosatetraenoic acids
  • Computer simulation
  • Fish gills
  • Free radicals
  • Hydroperoxides
  • Lipid peroxidation
  • Rat lung

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

Cite this

Oxidant stress inhibits the endogenous production of lipoxygenease metabolites in rat lungs and fish gills. / German, J. Bruce; Hu, Miao Lin.

In: Free Radical Biology and Medicine, Vol. 8, No. 5, 1990, p. 441-448.

Research output: Contribution to journalArticle

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