Overview of fluorescence lifetime imaging and metrology

Daniel S. Elson, Laura Marcu, Paul M.W. French

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


This chapter aims to present an overview of fluorescence lifetime imaging (FLIM) and metrology in the context of their biomedical applications, introducing the main approaches that are discussed in detail in subsequent chapters of this book. Before discussing fluorescence lifetime measurements, however, it is important to understand the phenomenon of fluorescence, of which a brief discussion is provided here, and the reader is directed to the classic textbook by Lakowicz (1999) for further details.

Original languageEnglish (US)
Title of host publicationFluorescence Lifetime Spectroscopy and Imaging
Subtitle of host publicationPrinciples and Applications in Biomedical Diagnostics
PublisherCRC Press
Number of pages15
ISBN (Electronic)9781439861684
ISBN (Print)9781439861677
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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