Overview of fluorescence lifetime imaging and metrology

Daniel S. Elson; Laura Marcu; Paul M.W. French

doi:10.1201/b17018

Overview of fluorescence lifetime imaging and metrology

Daniel S. Elson, Laura Marcu, Paul M.W. French

Biomedical Engineering

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3 Scopus citations

Abstract

This chapter aims to present an overview of fluorescence lifetime imaging (FLIM) and metrology in the context of their biomedical applications, introducing the main approaches that are discussed in detail in subsequent chapters of this book. Before discussing fluorescence lifetime measurements, however, it is important to understand the phenomenon of fluorescence, of which a brief discussion is provided here, and the reader is directed to the classic textbook by Lakowicz (1999) for further details.

Original language	English (US)
Title of host publication	Fluorescence Lifetime Spectroscopy and Imaging
Subtitle of host publication	Principles and Applications in Biomedical Diagnostics
Publisher	CRC Press
Pages	3-17
Number of pages	15
ISBN (Electronic)	9781439861684
ISBN (Print)	9781439861677
DOIs	https://doi.org/10.1201/b17018
State	Published - Jan 1 2014

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)
Biochemistry, Genetics and Molecular Biology(all)

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Overview of fluorescence lifetime imaging and metrology

Abstract

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