Abstract
This chapter aims to present an overview of fluorescence lifetime imaging (FLIM) and metrology in the context of their biomedical applications, introducing the main approaches that are discussed in detail in subsequent chapters of this book. Before discussing fluorescence lifetime measurements, however, it is important to understand the phenomenon of fluorescence, of which a brief discussion is provided here, and the reader is directed to the classic textbook by Lakowicz (1999) for further details.
Original language | English (US) |
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Title of host publication | Fluorescence Lifetime Spectroscopy and Imaging |
Subtitle of host publication | Principles and Applications in Biomedical Diagnostics |
Publisher | CRC Press |
Pages | 3-17 |
Number of pages | 15 |
ISBN (Electronic) | 9781439861684 |
ISBN (Print) | 9781439861677 |
DOIs | |
State | Published - Jan 1 2014 |
ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy(all)
- Biochemistry, Genetics and Molecular Biology(all)