Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation

Caitlin Kozel, Brytteny Thompson, Samantha Hustak, Chelsea Moore, Akio Nakashima, Chingakham Ranjit Singh, Megan Reid, Christian Cox, Evangelos Papadopoulos, Rafael E. Luna, Abbey Anderson, Hideaki Tagami, Hiroyuki Hiraishi, Emily Archer Slone, Ken Ichi Yoshino, Masayo Asano, Sarah Gillaspie, Jerome Nietfeld, Jean Pierre Perchellet, Stefan RothenburgHisao Masai, Gerhard Wagner, Alexander Beeser, Ushio Kikkawa, Sherry D. Fleming, Katsura Asano

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

ATF4 is a pro-oncogenic transcription factor whose translation is activated by eIF2 phosphorylation through delayed re-initiation involving two uORFs in the mRNA leader. However, in yeast, the effect of eIF2 phosphorylation can be mimicked by eIF5 overexpression, which turns eIF5 into translational inhibitor, thereby promoting translation of GCN4, the yeast ATF4 equivalent. Furthermore, regulatory protein termed eIF5-mimic protein (5MP) can bind eIF2 and inhibit general translation. Here, we show that 5MP1 overexpression in human cells leads to strong formation of 5MP1:eIF2 complex, nearly comparable to that of eIF5:eIF2 complex produced by eIF5 overexpression. Overexpression of eIF5, 5MP1 and 5MP2, the second human paralog, promotes ATF4 expression in certain types of human cells including fibrosarcoma. 5MP overexpression also induces ATF4 expression in Drosophila. The knockdown of 5MP1 in fibrosarcoma attenuates ATF4 expression and its tumor formation on nude mice. Since 5MP2 is overproduced in salivary mucoepidermoid carcinoma, we propose that overexpression of eIF5 and 5MP induces translation of ATF4 and potentially other genes with uORFs in their mRNA leaders through delayed re-initiation, thereby enhancing the survival of normal and cancer cells under stress conditions.

Original languageEnglish (US)
Pages (from-to)8704-8713
Number of pages10
JournalNucleic Acids Research
Volume44
Issue number18
DOIs
StatePublished - Oct 14 2016
Externally publishedYes

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Fibrosarcoma
Yeasts
Phosphorylation
Mucoepidermoid Carcinoma
Messenger RNA
Proteins
Nude Mice
Drosophila
Neoplasms
Transcription Factors
Survival
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation. / Kozel, Caitlin; Thompson, Brytteny; Hustak, Samantha; Moore, Chelsea; Nakashima, Akio; Singh, Chingakham Ranjit; Reid, Megan; Cox, Christian; Papadopoulos, Evangelos; Luna, Rafael E.; Anderson, Abbey; Tagami, Hideaki; Hiraishi, Hiroyuki; Slone, Emily Archer; Yoshino, Ken Ichi; Asano, Masayo; Gillaspie, Sarah; Nietfeld, Jerome; Perchellet, Jean Pierre; Rothenburg, Stefan; Masai, Hisao; Wagner, Gerhard; Beeser, Alexander; Kikkawa, Ushio; Fleming, Sherry D.; Asano, Katsura.

In: Nucleic Acids Research, Vol. 44, No. 18, 14.10.2016, p. 8704-8713.

Research output: Contribution to journalArticle

Kozel, C, Thompson, B, Hustak, S, Moore, C, Nakashima, A, Singh, CR, Reid, M, Cox, C, Papadopoulos, E, Luna, RE, Anderson, A, Tagami, H, Hiraishi, H, Slone, EA, Yoshino, KI, Asano, M, Gillaspie, S, Nietfeld, J, Perchellet, JP, Rothenburg, S, Masai, H, Wagner, G, Beeser, A, Kikkawa, U, Fleming, SD & Asano, K 2016, 'Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation', Nucleic Acids Research, vol. 44, no. 18, pp. 8704-8713. https://doi.org/10.1093/nar/gkw559
Kozel, Caitlin ; Thompson, Brytteny ; Hustak, Samantha ; Moore, Chelsea ; Nakashima, Akio ; Singh, Chingakham Ranjit ; Reid, Megan ; Cox, Christian ; Papadopoulos, Evangelos ; Luna, Rafael E. ; Anderson, Abbey ; Tagami, Hideaki ; Hiraishi, Hiroyuki ; Slone, Emily Archer ; Yoshino, Ken Ichi ; Asano, Masayo ; Gillaspie, Sarah ; Nietfeld, Jerome ; Perchellet, Jean Pierre ; Rothenburg, Stefan ; Masai, Hisao ; Wagner, Gerhard ; Beeser, Alexander ; Kikkawa, Ushio ; Fleming, Sherry D. ; Asano, Katsura. / Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation. In: Nucleic Acids Research. 2016 ; Vol. 44, No. 18. pp. 8704-8713.
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AU - Hustak, Samantha

AU - Moore, Chelsea

AU - Nakashima, Akio

AU - Singh, Chingakham Ranjit

AU - Reid, Megan

AU - Cox, Christian

AU - Papadopoulos, Evangelos

AU - Luna, Rafael E.

AU - Anderson, Abbey

AU - Tagami, Hideaki

AU - Hiraishi, Hiroyuki

AU - Slone, Emily Archer

AU - Yoshino, Ken Ichi

AU - Asano, Masayo

AU - Gillaspie, Sarah

AU - Nietfeld, Jerome

AU - Perchellet, Jean Pierre

AU - Rothenburg, Stefan

AU - Masai, Hisao

AU - Wagner, Gerhard

AU - Beeser, Alexander

AU - Kikkawa, Ushio

AU - Fleming, Sherry D.

AU - Asano, Katsura

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