Overexpression of cyclin DI is rare in human prostate carcinoma

Laura M. Gumbiner, Paul H. Gumerlock, Philip Mack, Sung Gil Chi, Ralph W deVere White, James L. Mohler, Thomas G. Pretlow, James V. Tricoli

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

BACKGROUND. Overexpression of cyclin D1 has been documented in a number of human cancers. Increased expression of cyclin D1 can contribute to cellular transformation and abnormal proliferation. METHODS. Quantitative RT- PCR and/or Western blot analysis were used to determine the level of cyclin D1 expression in 96 human prostate tumors, 15 benign prostate hyperplasias, 4 prostate cancer cell lines, and 3 xenografts. RESULTS. Our results demonstrate that 4.2% of the prostate tumors examined overexpressed cyclin D1 transcripts. In the cell lines, expression was normal, with the exception that reduced levels of cyclin D1 transcript and protein were observed in the DU145 cell line, as expected from cells with mutant RB. Normal levels of cyclin D1 were found in all xenograft tumors and BPH specimens examined. CONCLUSIONS. These data show that overexpression of cyclin D1 occurs rarely in human prostate tumors. However, when overexpression of cyclin D1 does occur, it may identify a subset of tumors with a different molecular biology.

Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalProstate
Volume38
Issue number1
DOIs
StatePublished - 1999

Keywords

  • Cyclin D1
  • Expression
  • Prostate cancer
  • Retinoblastoma
  • RT-PCR

ASJC Scopus subject areas

  • Urology

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