Outer-capsid P8 proteins of phytoreoviruses mediate secretion of assembled virus-like particles from insect cells

Naoyuki Miyazaki, Kyoji Hagiwara, Taiyun Wei, Hongyan Chen, Atsushi Nakagawa, Li Xing, R. Holland Cheng, Toshihiro Omura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Phytoreoviruses are composed of two concentric capsid layers that surround a viral genome. The capsids are formed mainly by the inner-capsid P3 protein and the outer-capsid P8 protein. During the infection of insect-vector cells, these play important roles in packaging the viral genome and the enzymes required for its transcription. P3 and P8 proteins, when co-expressed in Spodoptera frugiperda cells, co-localized in cells and were released as spherical clusters. In contrast P3 proteins expressed in the absence of P8 protein were associated with the cells when they were examined by confocal microscopy. Cryo-electron microscopy revealed that the secreted clusters, composed of P3 and P8 proteins, were double-layered virus-like particles that were indistinguishable from intact viral particles. Our results indicate that P8 proteins mediate the secretion of assembled virus-like particles from S. frugiperda insect cells and, therefore, most probably from insect-vector cells also.

Original languageEnglish (US)
Pages (from-to)2857-2861
Number of pages5
JournalJournal of General Virology
Volume91
Issue number11
DOIs
StatePublished - Nov 2010

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Reoviridae
Capsid Proteins
Virion
Insects
Insect Vectors
Spodoptera
Viral Genome
Capsid
Proteins
Cryoelectron Microscopy
Product Packaging
Confocal Microscopy
Enzymes
Infection

ASJC Scopus subject areas

  • Virology

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Outer-capsid P8 proteins of phytoreoviruses mediate secretion of assembled virus-like particles from insect cells. / Miyazaki, Naoyuki; Hagiwara, Kyoji; Wei, Taiyun; Chen, Hongyan; Nakagawa, Atsushi; Xing, Li; Cheng, R. Holland; Omura, Toshihiro.

In: Journal of General Virology, Vol. 91, No. 11, 11.2010, p. 2857-2861.

Research output: Contribution to journalArticle

Miyazaki, N, Hagiwara, K, Wei, T, Chen, H, Nakagawa, A, Xing, L, Cheng, RH & Omura, T 2010, 'Outer-capsid P8 proteins of phytoreoviruses mediate secretion of assembled virus-like particles from insect cells', Journal of General Virology, vol. 91, no. 11, pp. 2857-2861. https://doi.org/10.1099/vir.0.022012-0
Miyazaki, Naoyuki ; Hagiwara, Kyoji ; Wei, Taiyun ; Chen, Hongyan ; Nakagawa, Atsushi ; Xing, Li ; Cheng, R. Holland ; Omura, Toshihiro. / Outer-capsid P8 proteins of phytoreoviruses mediate secretion of assembled virus-like particles from insect cells. In: Journal of General Virology. 2010 ; Vol. 91, No. 11. pp. 2857-2861.
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