Abstract
Rapid deterioration of cold-induced thermoregulatory ability in aged male F344 rats is correlated with the spontaneous loss of body weight occurring near the end of the life span. This is accompanied by reduced brown fat thermogenic capacity [uncoupling protein (UCP) levels] and reduced NE (norepinephrine)-induced oxygen uptake (thermogenesis) of isolated brown fat cells (Fig. A). We tested if the latter reflects blunted cAMP production and/or fat mobilization. cAMP levels (Fig. B) and lipolysis (glycerol release; Fig. C) increased dose-dependently in response to NE, with no significant differences in weight stable (+) vs weight loss (O) 23-26 mo rats. Forskolin (50 μM)-stimulated cAMP and lipolysis were also comparable between groups. Thus, the reduced thermogenesis of brown fat cells from old F344 rats losing body weight likely reflects lower UCP levels per cell, rather than blunted ability to generate cAMP or mobilize fat (AG06665, AG05577).
| Original language | English (US) |
|---|---|
| Journal | FASEB Journal |
| Volume | 11 |
| Issue number | 3 |
| State | Published - 1997 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
O2 consumption, lipolysis, & cAMP in brown fat cells from aged F344 rats spontaneously losing body weight. / Gabaldón, A. M.; McDonald, R. B.; Horwitz, Barbara A.
In: FASEB Journal, Vol. 11, No. 3, 1997.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - O2 consumption, lipolysis, & cAMP in brown fat cells from aged F344 rats spontaneously losing body weight
AU - Gabaldón, A. M.
AU - McDonald, R. B.
AU - Horwitz, Barbara A
PY - 1997
Y1 - 1997
N2 - Rapid deterioration of cold-induced thermoregulatory ability in aged male F344 rats is correlated with the spontaneous loss of body weight occurring near the end of the life span. This is accompanied by reduced brown fat thermogenic capacity [uncoupling protein (UCP) levels] and reduced NE (norepinephrine)-induced oxygen uptake (thermogenesis) of isolated brown fat cells (Fig. A). We tested if the latter reflects blunted cAMP production and/or fat mobilization. cAMP levels (Fig. B) and lipolysis (glycerol release; Fig. C) increased dose-dependently in response to NE, with no significant differences in weight stable (+) vs weight loss (O) 23-26 mo rats. Forskolin (50 μM)-stimulated cAMP and lipolysis were also comparable between groups. Thus, the reduced thermogenesis of brown fat cells from old F344 rats losing body weight likely reflects lower UCP levels per cell, rather than blunted ability to generate cAMP or mobilize fat (AG06665, AG05577).
AB - Rapid deterioration of cold-induced thermoregulatory ability in aged male F344 rats is correlated with the spontaneous loss of body weight occurring near the end of the life span. This is accompanied by reduced brown fat thermogenic capacity [uncoupling protein (UCP) levels] and reduced NE (norepinephrine)-induced oxygen uptake (thermogenesis) of isolated brown fat cells (Fig. A). We tested if the latter reflects blunted cAMP production and/or fat mobilization. cAMP levels (Fig. B) and lipolysis (glycerol release; Fig. C) increased dose-dependently in response to NE, with no significant differences in weight stable (+) vs weight loss (O) 23-26 mo rats. Forskolin (50 μM)-stimulated cAMP and lipolysis were also comparable between groups. Thus, the reduced thermogenesis of brown fat cells from old F344 rats losing body weight likely reflects lower UCP levels per cell, rather than blunted ability to generate cAMP or mobilize fat (AG06665, AG05577).
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M3 - Article
VL - 11
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 3
ER -