O2 consumption, lipolysis, & cAMP in brown fat cells from aged F344 rats spontaneously losing body weight

A. M. Gabaldón, R. B. McDonald, Barbara A Horwitz

Research output: Contribution to journalArticle

Abstract

Rapid deterioration of cold-induced thermoregulatory ability in aged male F344 rats is correlated with the spontaneous loss of body weight occurring near the end of the life span. This is accompanied by reduced brown fat thermogenic capacity [uncoupling protein (UCP) levels] and reduced NE (norepinephrine)-induced oxygen uptake (thermogenesis) of isolated brown fat cells (Fig. A). We tested if the latter reflects blunted cAMP production and/or fat mobilization. cAMP levels (Fig. B) and lipolysis (glycerol release; Fig. C) increased dose-dependently in response to NE, with no significant differences in weight stable (+) vs weight loss (O) 23-26 mo rats. Forskolin (50 μM)-stimulated cAMP and lipolysis were also comparable between groups. Thus, the reduced thermogenesis of brown fat cells from old F344 rats losing body weight likely reflects lower UCP levels per cell, rather than blunted ability to generate cAMP or mobilize fat (AG06665, AG05577).

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

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Brown Adipocytes
brown adipose tissue
Thermogenesis
Lipolysis
Inbred F344 Rats
lipolysis
adipocytes
Rats
Norepinephrine
Fats
Body Weight
heat production
norepinephrine
Brown Adipose Tissue
body weight
rats
Colforsin
Glycerol
Weight Loss
forskolin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

O2 consumption, lipolysis, & cAMP in brown fat cells from aged F344 rats spontaneously losing body weight. / Gabaldón, A. M.; McDonald, R. B.; Horwitz, Barbara A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Gabaldón, A. M. ; McDonald, R. B. ; Horwitz, Barbara A. / O2 consumption, lipolysis, & cAMP in brown fat cells from aged F344 rats spontaneously losing body weight. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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