Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model

Mark Alvis, Peggy Lalor, Melissa K C Brown, Malahki R. Thorn, Jon E. Block, Samantha Hornby, Richard Berg, A Hari Reddi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In a subcutaneous implant rat model, Collagraft alone (n=8), Collagraft plus isologous bone marrow (n=8), and marrow alone (N=8) were evaluated. Twelve rats were euthanized at 11 days and 12 at 21 days. Explants were evaluated histologically for evidence of bone formation, and osteogenic activity was determined by an assay for alkaline phosphatase. Histological bone formation was absent in all groups at 11 days. At 21 days, in the Collagraft alone and bone marrow alone groups, no bone induction was noted. In contrast, 21-day specimens from the Collagraft plus bone marrow group showed newly formed bone. Alkaline phosphatase activity was negligible (0.05 units/mg) in all 11-day specimens. Activity in Collagraft plus bone marrow specimens at 21 days (0.38 units/mg) was significantly higher than any other group (P<.001 for all comparisons). This study demonstrates that Collagraft plus bone marrow is an osteoinductive matrix.

Original languageEnglish (US)
Pages (from-to)77-80
Number of pages4
JournalOrthopedics
Volume26
Issue number1
StatePublished - Jan 1 2003

Fingerprint

Minerals
Collagen
Bone Marrow
Osteogenesis
Alkaline Phosphatase
Bone and Bones
Collagraft

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Alvis, M., Lalor, P., Brown, M. K. C., Thorn, M. R., Block, J. E., Hornby, S., ... Reddi, A. H. (2003). Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model. Orthopedics, 26(1), 77-80.

Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model. / Alvis, Mark; Lalor, Peggy; Brown, Melissa K C; Thorn, Malahki R.; Block, Jon E.; Hornby, Samantha; Berg, Richard; Reddi, A Hari.

In: Orthopedics, Vol. 26, No. 1, 01.01.2003, p. 77-80.

Research output: Contribution to journalArticle

Alvis, M, Lalor, P, Brown, MKC, Thorn, MR, Block, JE, Hornby, S, Berg, R & Reddi, AH 2003, 'Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model', Orthopedics, vol. 26, no. 1, pp. 77-80.
Alvis M, Lalor P, Brown MKC, Thorn MR, Block JE, Hornby S et al. Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model. Orthopedics. 2003 Jan 1;26(1):77-80.
Alvis, Mark ; Lalor, Peggy ; Brown, Melissa K C ; Thorn, Malahki R. ; Block, Jon E. ; Hornby, Samantha ; Berg, Richard ; Reddi, A Hari. / Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model. In: Orthopedics. 2003 ; Vol. 26, No. 1. pp. 77-80.
@article{78e2e46be4ff42b58ee0387cc5c431d4,
title = "Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model",
abstract = "In a subcutaneous implant rat model, Collagraft alone (n=8), Collagraft plus isologous bone marrow (n=8), and marrow alone (N=8) were evaluated. Twelve rats were euthanized at 11 days and 12 at 21 days. Explants were evaluated histologically for evidence of bone formation, and osteogenic activity was determined by an assay for alkaline phosphatase. Histological bone formation was absent in all groups at 11 days. At 21 days, in the Collagraft alone and bone marrow alone groups, no bone induction was noted. In contrast, 21-day specimens from the Collagraft plus bone marrow group showed newly formed bone. Alkaline phosphatase activity was negligible (0.05 units/mg) in all 11-day specimens. Activity in Collagraft plus bone marrow specimens at 21 days (0.38 units/mg) was significantly higher than any other group (P<.001 for all comparisons). This study demonstrates that Collagraft plus bone marrow is an osteoinductive matrix.",
author = "Mark Alvis and Peggy Lalor and Brown, {Melissa K C} and Thorn, {Malahki R.} and Block, {Jon E.} and Samantha Hornby and Richard Berg and Reddi, {A Hari}",
year = "2003",
month = "1",
day = "1",
language = "English (US)",
volume = "26",
pages = "77--80",
journal = "Orthopedics",
issn = "0147-7447",
publisher = "Slack Incorporated",
number = "1",

}

TY - JOUR

T1 - Osteoinduction by a collagen mineral composite combined with isologous bone marrow in a subcutaneous rat model

AU - Alvis, Mark

AU - Lalor, Peggy

AU - Brown, Melissa K C

AU - Thorn, Malahki R.

AU - Block, Jon E.

AU - Hornby, Samantha

AU - Berg, Richard

AU - Reddi, A Hari

PY - 2003/1/1

Y1 - 2003/1/1

N2 - In a subcutaneous implant rat model, Collagraft alone (n=8), Collagraft plus isologous bone marrow (n=8), and marrow alone (N=8) were evaluated. Twelve rats were euthanized at 11 days and 12 at 21 days. Explants were evaluated histologically for evidence of bone formation, and osteogenic activity was determined by an assay for alkaline phosphatase. Histological bone formation was absent in all groups at 11 days. At 21 days, in the Collagraft alone and bone marrow alone groups, no bone induction was noted. In contrast, 21-day specimens from the Collagraft plus bone marrow group showed newly formed bone. Alkaline phosphatase activity was negligible (0.05 units/mg) in all 11-day specimens. Activity in Collagraft plus bone marrow specimens at 21 days (0.38 units/mg) was significantly higher than any other group (P<.001 for all comparisons). This study demonstrates that Collagraft plus bone marrow is an osteoinductive matrix.

AB - In a subcutaneous implant rat model, Collagraft alone (n=8), Collagraft plus isologous bone marrow (n=8), and marrow alone (N=8) were evaluated. Twelve rats were euthanized at 11 days and 12 at 21 days. Explants were evaluated histologically for evidence of bone formation, and osteogenic activity was determined by an assay for alkaline phosphatase. Histological bone formation was absent in all groups at 11 days. At 21 days, in the Collagraft alone and bone marrow alone groups, no bone induction was noted. In contrast, 21-day specimens from the Collagraft plus bone marrow group showed newly formed bone. Alkaline phosphatase activity was negligible (0.05 units/mg) in all 11-day specimens. Activity in Collagraft plus bone marrow specimens at 21 days (0.38 units/mg) was significantly higher than any other group (P<.001 for all comparisons). This study demonstrates that Collagraft plus bone marrow is an osteoinductive matrix.

UR - http://www.scopus.com/inward/record.url?scp=12244277377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12244277377&partnerID=8YFLogxK

M3 - Article

VL - 26

SP - 77

EP - 80

JO - Orthopedics

JF - Orthopedics

SN - 0147-7447

IS - 1

ER -