Abstract
Sympathetic neurons from perinatal rat pups extend only a single axon when maintained in culture in the absence of glia and serum. Exposure to recombinant osteogenic protein-1 (OP-1) selectively induces the formation of dendrites that correctly segregate and modify cytoskeletal and membrane proteins and form synaptic contacts of appropriate polarity. OP-1 requires nerve growth factor (NGF) as a cofactor, and, in the presence of optimal concentrations of NGF, OP1-induced dendritic growth from cultured perinatal neurons is comparable to that observed in situ. Sympathetic neuroblasts that had not formed dendrites in situ also responded to OP-1 in culture, indicating that OP-1 can cause de novo formation as well as regeneration of dendrites. These data imply that specific signals can regulate the development of neuronal shape and polarity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 597-605 |
| Number of pages | 9 |
| Journal | Neuron |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Osteogenic protein-1 induces dendritic growth in rat sympathetic neurons. / Lein, Pamela J; Johnson, Mary; Guo, Xin; Rueger, David; Higgins, Dennis.
In: Neuron, Vol. 15, No. 3, 1995, p. 597-605.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osteogenic protein-1 induces dendritic growth in rat sympathetic neurons
AU - Lein, Pamela J
AU - Johnson, Mary
AU - Guo, Xin
AU - Rueger, David
AU - Higgins, Dennis
PY - 1995
Y1 - 1995
N2 - Sympathetic neurons from perinatal rat pups extend only a single axon when maintained in culture in the absence of glia and serum. Exposure to recombinant osteogenic protein-1 (OP-1) selectively induces the formation of dendrites that correctly segregate and modify cytoskeletal and membrane proteins and form synaptic contacts of appropriate polarity. OP-1 requires nerve growth factor (NGF) as a cofactor, and, in the presence of optimal concentrations of NGF, OP1-induced dendritic growth from cultured perinatal neurons is comparable to that observed in situ. Sympathetic neuroblasts that had not formed dendrites in situ also responded to OP-1 in culture, indicating that OP-1 can cause de novo formation as well as regeneration of dendrites. These data imply that specific signals can regulate the development of neuronal shape and polarity.
AB - Sympathetic neurons from perinatal rat pups extend only a single axon when maintained in culture in the absence of glia and serum. Exposure to recombinant osteogenic protein-1 (OP-1) selectively induces the formation of dendrites that correctly segregate and modify cytoskeletal and membrane proteins and form synaptic contacts of appropriate polarity. OP-1 requires nerve growth factor (NGF) as a cofactor, and, in the presence of optimal concentrations of NGF, OP1-induced dendritic growth from cultured perinatal neurons is comparable to that observed in situ. Sympathetic neuroblasts that had not formed dendrites in situ also responded to OP-1 in culture, indicating that OP-1 can cause de novo formation as well as regeneration of dendrites. These data imply that specific signals can regulate the development of neuronal shape and polarity.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029082799&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(95)90148-5
DO - 10.1016/0896-6273(95)90148-5
M3 - Article
C2 - 7546739
AN - SCOPUS:0029082799
VL - 15
SP - 597
EP - 605
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 3
ER -