Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia

Dai Jung Chung, Kei Hayashi, Chrisoula A. Toupadakis, Alice Wong, Clare E Yellowley-genetos

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

The aim of this study was to compare the osteogenic and proliferative potential of canine mesenchymal stromal cells (cMSCs) derived from bone marrow (BM-cMSCs) and adipose tissue (AT-cMSCs). Proliferation potential was determined under varying oxygen tensions (1%, 5%, and 21% O 2). Effects of reduced oxygen levels on the osteogenic differentiation of AT-cMSCs were also investigated. AT-cMSCs proliferated at a significantly faster rate than BM-cMSCs, although both cell types showed robust osteogenic differentiation. Culture in 5% and 1% O 2 impaired proliferation in cMSC from both sources and osteogenic differentiation in AT-cMSCs. Our data suggests that AT-cMSCs might be more suitable for use in a clinical situation, where large cell numbers are required for bone repair, due to their rapid proliferation combined with robust osteogenic potential. Our data also suggests that the inhibitory effects of hypoxia on both cell proliferation and differentiation should be considered when using MSCs in a potentially hypoxic environment such as a fracture site.

Original languageEnglish (US)
Pages (from-to)66-75
Number of pages10
JournalResearch in Veterinary Science
Volume92
Issue number1
DOIs
StatePublished - Feb 2012

Keywords

  • Adipose tissue
  • Bone marrow
  • Canine mesenchymal stromal cell
  • Hypoxia
  • Osteogenic differentiation
  • Proliferation

ASJC Scopus subject areas

  • veterinary(all)

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