Abstract
An activity that recruits osteoclasts has been identified and partially characterized from bone matrix. Bone-derived osteoclast recruiting activity (BORA) was co-purified with osteogenin, a bone inductive protein. Osteogenin was extracted from bovine bone with 6 M urea and purified by chromatography on hydroxyapatite, heparin-Sepharose and Sephacryl S-200 gel filtration. The biologically active osteoclast formation-stimulating material was further purified by C18 reverse phase HPLC. BORA is obviously distinct from osteogenin and transforming growth factor β (TGF-β), since further purified osteogenin and pure TGF-β did not stimulate the formation of osteoclast-like cells. BORA (0.1-10 μg/ml) stimulated the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNC) in a dose-dependent manner. These multinucleated cells resorbed bone when cultured on bovine bone slices. The effect of BORA is primarily directed to differentiate osteoclast precursors, since it did not stimulate osteoclast function in in vitro resorption assay where disaggregated rat osteoclasts were cultured on bovine bone slices. However, after 24 h preincubation with 50 nM PTH in the mouse calvaria assay, BORA at 10 μg/ml significantly stimulated bone resorption.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-198 |
| Number of pages | 16 |
| Journal | Bone and Mineral |
| Volume | 25 |
| Issue number | 3 |
| State | Published - 1994 |
| Externally published | Yes |
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Keywords
- Bone matrix
- Osteoclast recruitment
- Osteogenin
- TGF-β
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Surgery
Cite this
Osteoclast recruiting activity in bone matrix. / Hentunen, T. A.; Cunningham, N. S.; Vuolteenaho, O.; Reddi, A Hari; Vaananen, H. K.
In: Bone and Mineral, Vol. 25, No. 3, 1994, p. 183-198.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osteoclast recruiting activity in bone matrix
AU - Hentunen, T. A.
AU - Cunningham, N. S.
AU - Vuolteenaho, O.
AU - Reddi, A Hari
AU - Vaananen, H. K.
PY - 1994
Y1 - 1994
N2 - An activity that recruits osteoclasts has been identified and partially characterized from bone matrix. Bone-derived osteoclast recruiting activity (BORA) was co-purified with osteogenin, a bone inductive protein. Osteogenin was extracted from bovine bone with 6 M urea and purified by chromatography on hydroxyapatite, heparin-Sepharose and Sephacryl S-200 gel filtration. The biologically active osteoclast formation-stimulating material was further purified by C18 reverse phase HPLC. BORA is obviously distinct from osteogenin and transforming growth factor β (TGF-β), since further purified osteogenin and pure TGF-β did not stimulate the formation of osteoclast-like cells. BORA (0.1-10 μg/ml) stimulated the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNC) in a dose-dependent manner. These multinucleated cells resorbed bone when cultured on bovine bone slices. The effect of BORA is primarily directed to differentiate osteoclast precursors, since it did not stimulate osteoclast function in in vitro resorption assay where disaggregated rat osteoclasts were cultured on bovine bone slices. However, after 24 h preincubation with 50 nM PTH in the mouse calvaria assay, BORA at 10 μg/ml significantly stimulated bone resorption.
AB - An activity that recruits osteoclasts has been identified and partially characterized from bone matrix. Bone-derived osteoclast recruiting activity (BORA) was co-purified with osteogenin, a bone inductive protein. Osteogenin was extracted from bovine bone with 6 M urea and purified by chromatography on hydroxyapatite, heparin-Sepharose and Sephacryl S-200 gel filtration. The biologically active osteoclast formation-stimulating material was further purified by C18 reverse phase HPLC. BORA is obviously distinct from osteogenin and transforming growth factor β (TGF-β), since further purified osteogenin and pure TGF-β did not stimulate the formation of osteoclast-like cells. BORA (0.1-10 μg/ml) stimulated the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNC) in a dose-dependent manner. These multinucleated cells resorbed bone when cultured on bovine bone slices. The effect of BORA is primarily directed to differentiate osteoclast precursors, since it did not stimulate osteoclast function in in vitro resorption assay where disaggregated rat osteoclasts were cultured on bovine bone slices. However, after 24 h preincubation with 50 nM PTH in the mouse calvaria assay, BORA at 10 μg/ml significantly stimulated bone resorption.
KW - Bone matrix
KW - Osteoclast recruitment
KW - Osteogenin
KW - TGF-β
UR - http://www.scopus.com/inward/record.url?scp=0028178118&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028178118&partnerID=8YFLogxK
M3 - Article
VL - 25
SP - 183
EP - 198
JO - Bone and Mineral
JF - Bone and Mineral
SN - 0169-6009
IS - 3
ER -