Osteoclast differentiation and bone resorption in multicentric reticulohistiocytosis

Iannis Adamopoulos, Paul B. Wordsworth, James R. Edwards, David J. Ferguson, Nicholas A. Athanasou

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Multicentric reticulohistiocytosis (MR) is a systemic disease of unknown cause characterized by the presence of a heavy macrophage infiltrate in skin and synovial tissues and the development of an erosive polyarthritis. The synovial fluid in MR is known to contain numerous mononuclear cells. In this study, we have determined whether these cells contribute to joint destruction in MR by differentiating them into osteoclasts. Synovial fluid mononuclear cells were isolated from the knee joint of a 44-year-old male with MR. These cells were cultured with various combinations of macrophage-colony stimulating factor, receptor activator for nuclear factor κB ligand (RANKL), tumor necrosis factor α, interleukin-1α, osteoprotegerin, and zoledronate. Osteoclast differentiation was assessed by expression of tartrate-resistant acid phosphatase, vitronectin receptor, and the extent of lacunar resorption. Most MR synovial fluid mononuclear cells expressed a macrophage phenotype (CD14+, CD68+, HLA-DR+, CD11b+). Extensive osteoclast formation and lacunar resorption were noted in macrophage-colony stimulating factor/RANKL-treated cell cultures. MR synovial fluid contained increased tumor necrosis factor α and decreased osteoprotegerin compared with osteoarthritis synovial fluid. Lacunar resorption was inhibited in cultures containing zoledronate. Pamidronate treatment of the patient also reduced the number of synovial fluid macrophages and resulted in less osteoclast formation and lacunar resorption. MR synovial fluid contains numerous macrophages that are capable of differentiating into osteoclasts by the RANKL signaling pathway. Inhibitors of osteoclast formation and resorption activity may be of use in preventing the severe joint destruction that commonly occurs in MR.

Original languageEnglish (US)
Pages (from-to)1176-1185
Number of pages10
JournalHuman Pathology
Volume37
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Synovial Fluid
Osteoclasts
Bone Resorption
zoledronic acid
Macrophages
Osteoprotegerin
pamidronate
Tumor Necrosis Factor-alpha
Joints
Integrin alphaVbeta3
Macrophage Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor
HLA-DR Antigens
Knee Joint
Interleukin-1
Osteoarthritis
Arthritis
Cultured Cells
Cell Culture Techniques
Ligands

Keywords

  • Bone resorption
  • Differentiation
  • Multicentric reticulohistiocytosis
  • Osteoclasts

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Osteoclast differentiation and bone resorption in multicentric reticulohistiocytosis. / Adamopoulos, Iannis; Wordsworth, Paul B.; Edwards, James R.; Ferguson, David J.; Athanasou, Nicholas A.

In: Human Pathology, Vol. 37, No. 9, 09.2006, p. 1176-1185.

Research output: Contribution to journalArticle

Adamopoulos, Iannis ; Wordsworth, Paul B. ; Edwards, James R. ; Ferguson, David J. ; Athanasou, Nicholas A. / Osteoclast differentiation and bone resorption in multicentric reticulohistiocytosis. In: Human Pathology. 2006 ; Vol. 37, No. 9. pp. 1176-1185.
@article{3d4ba08651ca4e1aa60ccdb7b3c0ada1,
title = "Osteoclast differentiation and bone resorption in multicentric reticulohistiocytosis",
abstract = "Multicentric reticulohistiocytosis (MR) is a systemic disease of unknown cause characterized by the presence of a heavy macrophage infiltrate in skin and synovial tissues and the development of an erosive polyarthritis. The synovial fluid in MR is known to contain numerous mononuclear cells. In this study, we have determined whether these cells contribute to joint destruction in MR by differentiating them into osteoclasts. Synovial fluid mononuclear cells were isolated from the knee joint of a 44-year-old male with MR. These cells were cultured with various combinations of macrophage-colony stimulating factor, receptor activator for nuclear factor κB ligand (RANKL), tumor necrosis factor α, interleukin-1α, osteoprotegerin, and zoledronate. Osteoclast differentiation was assessed by expression of tartrate-resistant acid phosphatase, vitronectin receptor, and the extent of lacunar resorption. Most MR synovial fluid mononuclear cells expressed a macrophage phenotype (CD14+, CD68+, HLA-DR+, CD11b+). Extensive osteoclast formation and lacunar resorption were noted in macrophage-colony stimulating factor/RANKL-treated cell cultures. MR synovial fluid contained increased tumor necrosis factor α and decreased osteoprotegerin compared with osteoarthritis synovial fluid. Lacunar resorption was inhibited in cultures containing zoledronate. Pamidronate treatment of the patient also reduced the number of synovial fluid macrophages and resulted in less osteoclast formation and lacunar resorption. MR synovial fluid contains numerous macrophages that are capable of differentiating into osteoclasts by the RANKL signaling pathway. Inhibitors of osteoclast formation and resorption activity may be of use in preventing the severe joint destruction that commonly occurs in MR.",
keywords = "Bone resorption, Differentiation, Multicentric reticulohistiocytosis, Osteoclasts",
author = "Iannis Adamopoulos and Wordsworth, {Paul B.} and Edwards, {James R.} and Ferguson, {David J.} and Athanasou, {Nicholas A.}",
year = "2006",
month = "9",
doi = "10.1016/j.humpath.2006.04.007",
language = "English (US)",
volume = "37",
pages = "1176--1185",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "9",

}

TY - JOUR

T1 - Osteoclast differentiation and bone resorption in multicentric reticulohistiocytosis

AU - Adamopoulos, Iannis

AU - Wordsworth, Paul B.

AU - Edwards, James R.

AU - Ferguson, David J.

AU - Athanasou, Nicholas A.

PY - 2006/9

Y1 - 2006/9

N2 - Multicentric reticulohistiocytosis (MR) is a systemic disease of unknown cause characterized by the presence of a heavy macrophage infiltrate in skin and synovial tissues and the development of an erosive polyarthritis. The synovial fluid in MR is known to contain numerous mononuclear cells. In this study, we have determined whether these cells contribute to joint destruction in MR by differentiating them into osteoclasts. Synovial fluid mononuclear cells were isolated from the knee joint of a 44-year-old male with MR. These cells were cultured with various combinations of macrophage-colony stimulating factor, receptor activator for nuclear factor κB ligand (RANKL), tumor necrosis factor α, interleukin-1α, osteoprotegerin, and zoledronate. Osteoclast differentiation was assessed by expression of tartrate-resistant acid phosphatase, vitronectin receptor, and the extent of lacunar resorption. Most MR synovial fluid mononuclear cells expressed a macrophage phenotype (CD14+, CD68+, HLA-DR+, CD11b+). Extensive osteoclast formation and lacunar resorption were noted in macrophage-colony stimulating factor/RANKL-treated cell cultures. MR synovial fluid contained increased tumor necrosis factor α and decreased osteoprotegerin compared with osteoarthritis synovial fluid. Lacunar resorption was inhibited in cultures containing zoledronate. Pamidronate treatment of the patient also reduced the number of synovial fluid macrophages and resulted in less osteoclast formation and lacunar resorption. MR synovial fluid contains numerous macrophages that are capable of differentiating into osteoclasts by the RANKL signaling pathway. Inhibitors of osteoclast formation and resorption activity may be of use in preventing the severe joint destruction that commonly occurs in MR.

AB - Multicentric reticulohistiocytosis (MR) is a systemic disease of unknown cause characterized by the presence of a heavy macrophage infiltrate in skin and synovial tissues and the development of an erosive polyarthritis. The synovial fluid in MR is known to contain numerous mononuclear cells. In this study, we have determined whether these cells contribute to joint destruction in MR by differentiating them into osteoclasts. Synovial fluid mononuclear cells were isolated from the knee joint of a 44-year-old male with MR. These cells were cultured with various combinations of macrophage-colony stimulating factor, receptor activator for nuclear factor κB ligand (RANKL), tumor necrosis factor α, interleukin-1α, osteoprotegerin, and zoledronate. Osteoclast differentiation was assessed by expression of tartrate-resistant acid phosphatase, vitronectin receptor, and the extent of lacunar resorption. Most MR synovial fluid mononuclear cells expressed a macrophage phenotype (CD14+, CD68+, HLA-DR+, CD11b+). Extensive osteoclast formation and lacunar resorption were noted in macrophage-colony stimulating factor/RANKL-treated cell cultures. MR synovial fluid contained increased tumor necrosis factor α and decreased osteoprotegerin compared with osteoarthritis synovial fluid. Lacunar resorption was inhibited in cultures containing zoledronate. Pamidronate treatment of the patient also reduced the number of synovial fluid macrophages and resulted in less osteoclast formation and lacunar resorption. MR synovial fluid contains numerous macrophages that are capable of differentiating into osteoclasts by the RANKL signaling pathway. Inhibitors of osteoclast formation and resorption activity may be of use in preventing the severe joint destruction that commonly occurs in MR.

KW - Bone resorption

KW - Differentiation

KW - Multicentric reticulohistiocytosis

KW - Osteoclasts

UR - http://www.scopus.com/inward/record.url?scp=33747826655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747826655&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2006.04.007

DO - 10.1016/j.humpath.2006.04.007

M3 - Article

C2 - 16938523

AN - SCOPUS:33747826655

VL - 37

SP - 1176

EP - 1185

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 9

ER -