Ortho-substituted 2,2',3,5',6-pentachlorobiphenyl (PCB 95) alters rat hippocampal ryanodine receptors and neuroplasticity in vitro: Evidence for altered hippocampal function

P. W. Wong, R. M. Joy, Timothy E Albertson, S. L. Schantz, Isaac N Pessah

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Abstract

The effects of PCBs on hippocampal function were studied in vitro, by radioligand-receptor binding analysis and electrophysiological measurements of the hippocampal slice preparation. [3H]Ryanodine, a conformation- sensitive probe for ryanodine receptors, was employed to determine how PCBs influence specific high-affinity occupancy to receptors found in microsomes isolated from rat hippocampus. PCB 95 (2,2',3,5',6-pentachlorobiphenyl) exhibited a dose-dependent enhancement of [3H]ryanodine receptor occupancy with an EC50 of 12 μM. In contrast, PCB 66 (2,3',4,4'-tetrachlorobiphenyl) showed no activity toward ryanodine receptors, up to its solubility limit (≤200 μM). Population spike (PS) and excitatory postsynaptic potential (EPSP) responses were recorded from striatum pyramidale of the CA1 region, which were generated from single pulse orthodromic stimulation of Schaffer collateral/commissural (SC/C) fibers at striatum radiatum of the hippocampal slice preparation. After the introduction of PCB 95 to the perfusion medium, PCB 95 depressed PS amplitude, especially at high stimulus intensities. Significant reductions in PS and EPSP maxima were seen, even after induction of long term potentiation, a model of neuroplasticity. However, these actions were not observed with PCB 66 which lacks ryanodine receptor activity, implicating a ryanodine receptor-mediated mechanism in the general depression of pyramidal cell excitability seen with PCB 95. Taken together, these results reveal a novel, arylhydrocarbon (Ah) receptor-independent, mechanism by which PCB 95 alters neuronal Ca2+ signaling and neuroplasticity in adult brain.

Original languageEnglish (US)
Pages (from-to)443-456
Number of pages14
JournalNeuroToxicology
Volume18
Issue number2
StatePublished - 1997

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Ryanodine Receptor Calcium Release Channel
Neuronal Plasticity
Rats
Polychlorinated Biphenyls
Excitatory Postsynaptic Potentials
Hippocampus
Population
Ryanodine
Long-Term Potentiation
Pyramidal Cells
Microsomes
Solubility
Conformations
In Vitro Techniques
2,2',3,5',6-pentachlorobiphenyl
Brain
Perfusion
Fibers

Keywords

  • Long term potentiation
  • Polychlorinated biphenyl
  • Ryanodine receptor

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Toxicology

Cite this

@article{477b70fae4f643f09e31b4a14e6f6c17,
title = "Ortho-substituted 2,2',3,5',6-pentachlorobiphenyl (PCB 95) alters rat hippocampal ryanodine receptors and neuroplasticity in vitro: Evidence for altered hippocampal function",
abstract = "The effects of PCBs on hippocampal function were studied in vitro, by radioligand-receptor binding analysis and electrophysiological measurements of the hippocampal slice preparation. [3H]Ryanodine, a conformation- sensitive probe for ryanodine receptors, was employed to determine how PCBs influence specific high-affinity occupancy to receptors found in microsomes isolated from rat hippocampus. PCB 95 (2,2',3,5',6-pentachlorobiphenyl) exhibited a dose-dependent enhancement of [3H]ryanodine receptor occupancy with an EC50 of 12 μM. In contrast, PCB 66 (2,3',4,4'-tetrachlorobiphenyl) showed no activity toward ryanodine receptors, up to its solubility limit (≤200 μM). Population spike (PS) and excitatory postsynaptic potential (EPSP) responses were recorded from striatum pyramidale of the CA1 region, which were generated from single pulse orthodromic stimulation of Schaffer collateral/commissural (SC/C) fibers at striatum radiatum of the hippocampal slice preparation. After the introduction of PCB 95 to the perfusion medium, PCB 95 depressed PS amplitude, especially at high stimulus intensities. Significant reductions in PS and EPSP maxima were seen, even after induction of long term potentiation, a model of neuroplasticity. However, these actions were not observed with PCB 66 which lacks ryanodine receptor activity, implicating a ryanodine receptor-mediated mechanism in the general depression of pyramidal cell excitability seen with PCB 95. Taken together, these results reveal a novel, arylhydrocarbon (Ah) receptor-independent, mechanism by which PCB 95 alters neuronal Ca2+ signaling and neuroplasticity in adult brain.",
keywords = "Long term potentiation, Polychlorinated biphenyl, Ryanodine receptor",
author = "Wong, {P. W.} and Joy, {R. M.} and Albertson, {Timothy E} and Schantz, {S. L.} and Pessah, {Isaac N}",
year = "1997",
language = "English (US)",
volume = "18",
pages = "443--456",
journal = "NeuroToxicology",
issn = "0161-813X",
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TY - JOUR

T1 - Ortho-substituted 2,2',3,5',6-pentachlorobiphenyl (PCB 95) alters rat hippocampal ryanodine receptors and neuroplasticity in vitro

T2 - Evidence for altered hippocampal function

AU - Wong, P. W.

AU - Joy, R. M.

AU - Albertson, Timothy E

AU - Schantz, S. L.

AU - Pessah, Isaac N

PY - 1997

Y1 - 1997

N2 - The effects of PCBs on hippocampal function were studied in vitro, by radioligand-receptor binding analysis and electrophysiological measurements of the hippocampal slice preparation. [3H]Ryanodine, a conformation- sensitive probe for ryanodine receptors, was employed to determine how PCBs influence specific high-affinity occupancy to receptors found in microsomes isolated from rat hippocampus. PCB 95 (2,2',3,5',6-pentachlorobiphenyl) exhibited a dose-dependent enhancement of [3H]ryanodine receptor occupancy with an EC50 of 12 μM. In contrast, PCB 66 (2,3',4,4'-tetrachlorobiphenyl) showed no activity toward ryanodine receptors, up to its solubility limit (≤200 μM). Population spike (PS) and excitatory postsynaptic potential (EPSP) responses were recorded from striatum pyramidale of the CA1 region, which were generated from single pulse orthodromic stimulation of Schaffer collateral/commissural (SC/C) fibers at striatum radiatum of the hippocampal slice preparation. After the introduction of PCB 95 to the perfusion medium, PCB 95 depressed PS amplitude, especially at high stimulus intensities. Significant reductions in PS and EPSP maxima were seen, even after induction of long term potentiation, a model of neuroplasticity. However, these actions were not observed with PCB 66 which lacks ryanodine receptor activity, implicating a ryanodine receptor-mediated mechanism in the general depression of pyramidal cell excitability seen with PCB 95. Taken together, these results reveal a novel, arylhydrocarbon (Ah) receptor-independent, mechanism by which PCB 95 alters neuronal Ca2+ signaling and neuroplasticity in adult brain.

AB - The effects of PCBs on hippocampal function were studied in vitro, by radioligand-receptor binding analysis and electrophysiological measurements of the hippocampal slice preparation. [3H]Ryanodine, a conformation- sensitive probe for ryanodine receptors, was employed to determine how PCBs influence specific high-affinity occupancy to receptors found in microsomes isolated from rat hippocampus. PCB 95 (2,2',3,5',6-pentachlorobiphenyl) exhibited a dose-dependent enhancement of [3H]ryanodine receptor occupancy with an EC50 of 12 μM. In contrast, PCB 66 (2,3',4,4'-tetrachlorobiphenyl) showed no activity toward ryanodine receptors, up to its solubility limit (≤200 μM). Population spike (PS) and excitatory postsynaptic potential (EPSP) responses were recorded from striatum pyramidale of the CA1 region, which were generated from single pulse orthodromic stimulation of Schaffer collateral/commissural (SC/C) fibers at striatum radiatum of the hippocampal slice preparation. After the introduction of PCB 95 to the perfusion medium, PCB 95 depressed PS amplitude, especially at high stimulus intensities. Significant reductions in PS and EPSP maxima were seen, even after induction of long term potentiation, a model of neuroplasticity. However, these actions were not observed with PCB 66 which lacks ryanodine receptor activity, implicating a ryanodine receptor-mediated mechanism in the general depression of pyramidal cell excitability seen with PCB 95. Taken together, these results reveal a novel, arylhydrocarbon (Ah) receptor-independent, mechanism by which PCB 95 alters neuronal Ca2+ signaling and neuroplasticity in adult brain.

KW - Long term potentiation

KW - Polychlorinated biphenyl

KW - Ryanodine receptor

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M3 - Article

C2 - 9291493

AN - SCOPUS:0030854174

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SP - 443

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JF - NeuroToxicology

SN - 0161-813X

IS - 2

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