Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae

Margit Foss, Francis J. McNally, Patricia Laurenson, Jasper Rine

Research output: Contribution to journalArticle

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Abstract

In Saccharomyces cerevisiae, the HMR-E silencer blocks site-specific interactions between proteins and their recognition sequences in the vicinity of the silencer. Silencer function is correlated with the firing of an origin of replication at HMR-E. An essential gene with a role in transcriptional silencing was identified by means of a screen for mutations affecting expression of HMR. This gene, known as ORC2, was shown to encode a component of the origin recognition complex that binds yeast origins of replication. A temperature-sensitive mutation in ORC2 disrupted silencing in cells grown at the permissive temperature. At the restrictive temperature, the orc2-1 mutation caused cell cycle arrest at a point in the cell cycle indicative of blocks in DNA replication. The orc2-1 mutation also resulted in the enhanced mitotic loss of a plasmid, suggestive of a defect in replication. These results provide strong evidence for an in vivo role of ORC in both chromosomal replication and silencing, and provide a link between the mechanism of silencing and DNA replication.

Original languageEnglish (US)
Pages (from-to)1838-1844
Number of pages7
JournalScience
Volume262
Issue number5141
StatePublished - Dec 17 1993

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    Foss, M., McNally, F. J., Laurenson, P., & Rine, J. (1993). Origin recognition complex (ORC) in transcriptional silencing and DNA replication in S. cerevisiae. Science, 262(5141), 1838-1844.