Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex

Lee A. Shapiro, Kwan Ng, Richard Kinyamu, Patricia Whitaker-Azmitia, Eldon E. Geisert, Mathew Blurton-Jones, Qun Yong Zhou, Charles E. Ribak

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Newly generated neurons are continuously added to the olfactory epithelium and olfactory bulbs of adult mammals. Studies also report newly generated neurons in the piriform cortex, the primary cortical projection site of the olfactory bulbs. The current study used BrdU-injection paradigms, and in vivo and in vitro DiI tracing methods to address three fundamental issues of these cells: their origin, migratory route and fate. The results show that 1 day after a BrdU-injection, BrdU/DCX double-labeled cells appear deep to the ventricular subependyma, within the white matter. Such cells appear further ventral and caudal in the ensuing days, first appearing in the rostral piriform cortex of mice at 2 days after the BrdU-injection, and at 4 days in the rat. In the caudal piriform cortex, BrdU/DCX labeled cells first appear at 4 days after the injection in mice and 7 days in rats. The time it takes for these cells to appear in the piriform cortex and the temporal distribution pattern suggest that they migrate from outside this region. DiI tracing methods confirmed a migratory route to the piriform cortex from the ventricular subependyma. The presence of BrdU/NeuN labeled cells as early as 7 days after a BrdU injection in mice and 10 days in the rat and lasting as long as 41 days indicates that some of these cells have extended survival durations in the adult piriform cortex.

Original languageEnglish (US)
Pages (from-to)133-148
Number of pages16
JournalBrain Structure and Function
Volume212
Issue number2
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Bromodeoxyuridine
Rodentia
Neurons
Injections
Olfactory Bulb
Olfactory Mucosa
Piriform Cortex
Mammals

Keywords

  • Bromodeoxyuridine
  • DiI tracing
  • Doublecortin
  • Neurogenesis
  • Olfactory system

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience(all)
  • Histology

Cite this

Shapiro, L. A., Ng, K., Kinyamu, R., Whitaker-Azmitia, P., Geisert, E. E., Blurton-Jones, M., ... Ribak, C. E. (2007). Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex. Brain Structure and Function, 212(2), 133-148. https://doi.org/10.1007/s00429-007-0151-3

Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex. / Shapiro, Lee A.; Ng, Kwan; Kinyamu, Richard; Whitaker-Azmitia, Patricia; Geisert, Eldon E.; Blurton-Jones, Mathew; Zhou, Qun Yong; Ribak, Charles E.

In: Brain Structure and Function, Vol. 212, No. 2, 01.09.2007, p. 133-148.

Research output: Contribution to journalArticle

Shapiro, LA, Ng, K, Kinyamu, R, Whitaker-Azmitia, P, Geisert, EE, Blurton-Jones, M, Zhou, QY & Ribak, CE 2007, 'Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex', Brain Structure and Function, vol. 212, no. 2, pp. 133-148. https://doi.org/10.1007/s00429-007-0151-3
Shapiro, Lee A. ; Ng, Kwan ; Kinyamu, Richard ; Whitaker-Azmitia, Patricia ; Geisert, Eldon E. ; Blurton-Jones, Mathew ; Zhou, Qun Yong ; Ribak, Charles E. / Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex. In: Brain Structure and Function. 2007 ; Vol. 212, No. 2. pp. 133-148.
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