Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes

Olga G. Shcherbakova, Carl M. Hurt, Yang Kevin Xiang, Mark L. Dell'Acqua, Qi Zhang, Richard W. Tsien, Brian K. Kobilka

Research output: Contribution to journalArticle

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Abstract

The sympathetic nervous system regulates cardiac function through the activation of adrenergic receptors (ARs). β1 and β2ARs are the primary sympathetic receptors in the heart and play different roles in regulating cardiac contractile function and remodeling in response to injury. In this study, we examine the targeting and trafficking of β1 and β2ARs at cardiac sympathetic synapses in vitro. Sympathetic neurons form functional synapses with neonatal cardiac myocytes in culture. The myocyte membrane develops into specialized zones that surround contacting axons and contain accumulations of the scaffold proteins SAP97 and AKAP79/150 but are deficient in caveolin-3. The β1ARs are enriched within these zones, whereas β2ARs are excluded from them after stimulation of neuronal activity. The results indicate that specialized signaling domains are organized in cardiac myocytes at sites of contact with sympathetic neurons and that these domains are likely to play a role in the subtype-specific regulation of cardiac function by β1 and β2ARs in vivo.

Original languageEnglish (US)
Pages (from-to)521-533
Number of pages13
JournalJournal of Cell Biology
Volume176
Issue number4
DOIs
StatePublished - Feb 12 2007
Externally publishedYes

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Cardiac Myocytes
Synapses
Adrenergic Receptors
Caveolin 3
Neurons
Sympathetic Nervous System
Muscle Cells
Axons
Membranes
Wounds and Injuries
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Cell Biology

Cite this

Shcherbakova, O. G., Hurt, C. M., Xiang, Y. K., Dell'Acqua, M. L., Zhang, Q., Tsien, R. W., & Kobilka, B. K. (2007). Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes. Journal of Cell Biology, 176(4), 521-533. https://doi.org/10.1083/jcb.200604167

Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes. / Shcherbakova, Olga G.; Hurt, Carl M.; Xiang, Yang Kevin; Dell'Acqua, Mark L.; Zhang, Qi; Tsien, Richard W.; Kobilka, Brian K.

In: Journal of Cell Biology, Vol. 176, No. 4, 12.02.2007, p. 521-533.

Research output: Contribution to journalArticle

Shcherbakova, OG, Hurt, CM, Xiang, YK, Dell'Acqua, ML, Zhang, Q, Tsien, RW & Kobilka, BK 2007, 'Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes', Journal of Cell Biology, vol. 176, no. 4, pp. 521-533. https://doi.org/10.1083/jcb.200604167
Shcherbakova, Olga G. ; Hurt, Carl M. ; Xiang, Yang Kevin ; Dell'Acqua, Mark L. ; Zhang, Qi ; Tsien, Richard W. ; Kobilka, Brian K. / Organization of β-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes. In: Journal of Cell Biology. 2007 ; Vol. 176, No. 4. pp. 521-533.
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