Organization and quantitative analysis of kainate receptor subunit GluR5-7 immunoreactivity in monkey hippocampus

Paul F. Good; George W. Huntley; Scott W. Rogers; Stephen F. Heinemann; John Morrison

doi:10.1016/0006-8993(93)90102-S

Organization and quantitative analysis of kainate receptor subunit GluR5-7 immunoreactivity in monkey hippocampus

Paul F. Good, George W. Huntley, Scott W. Rogers, Stephen F. Heinemann, John Morrison

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

A monoclonal antibody specific for GluR5-7 (mAb-4F5) has been used to characterize the distribution of kainate class glutamate receptor subunits in monkey hippocampus. Immunolabeled neurons were present in all subfields of the hippocampus as well as the dentate gyrus and subiculum. Quantitative immunofluorescence analysis by confocal microscopy demonstrated differential levels of immunoreactivity such that the highest intensities were in neurons within CA1 and subiculum as compared with those within CA3 or dentate gyrus. The regional differences in levels of subunit immunoreactivity correlate with the relative vulnerability of hippocampal neurons in several neurodegenerative disorders.

Original language	English (US)
Pages (from-to)	347-353
Number of pages	7
Journal	Brain Research
Volume	624
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(93)90102-S
State	Published - Oct 8 1993
Externally published	Yes

Keywords

Confocal microscopy
Excitatory amino acid
Glutamate receptor
Neurodegeneration
Primate
Quantitative neuroanatomy

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

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10.1016/0006-8993(93)90102-S

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abstract = "A monoclonal antibody specific for GluR5-7 (mAb-4F5) has been used to characterize the distribution of kainate class glutamate receptor subunits in monkey hippocampus. Immunolabeled neurons were present in all subfields of the hippocampus as well as the dentate gyrus and subiculum. Quantitative immunofluorescence analysis by confocal microscopy demonstrated differential levels of immunoreactivity such that the highest intensities were in neurons within CA1 and subiculum as compared with those within CA3 or dentate gyrus. The regional differences in levels of subunit immunoreactivity correlate with the relative vulnerability of hippocampal neurons in several neurodegenerative disorders.",

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T1 - Organization and quantitative analysis of kainate receptor subunit GluR5-7 immunoreactivity in monkey hippocampus

AU - Good, Paul F.

AU - Huntley, George W.

AU - Rogers, Scott W.

AU - Heinemann, Stephen F.

AU - Morrison, John

PY - 1993/10/8

Y1 - 1993/10/8

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AB - A monoclonal antibody specific for GluR5-7 (mAb-4F5) has been used to characterize the distribution of kainate class glutamate receptor subunits in monkey hippocampus. Immunolabeled neurons were present in all subfields of the hippocampus as well as the dentate gyrus and subiculum. Quantitative immunofluorescence analysis by confocal microscopy demonstrated differential levels of immunoreactivity such that the highest intensities were in neurons within CA1 and subiculum as compared with those within CA3 or dentate gyrus. The regional differences in levels of subunit immunoreactivity correlate with the relative vulnerability of hippocampal neurons in several neurodegenerative disorders.

KW - Confocal microscopy

KW - Excitatory amino acid

KW - Glutamate receptor

KW - Neurodegeneration

KW - Primate

KW - Quantitative neuroanatomy

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Organization and quantitative analysis of kainate receptor subunit GluR5-7 immunoreactivity in monkey hippocampus

Abstract

Keywords

ASJC Scopus subject areas

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