Organization and function of a dioxin-responsive enhancer

Joan M. Fisher, Lena Wu, Michael S. Denison, James P. Whitlock

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

The dioxin-responsive enhancer upstream of the CYP1A1 gene contains four copies of the recognition motif for the liganded Ah receptor. The results of deletion analyses, linker-scanning analyses, and the analysis of individual enhancer subdomains reveal that each copy of the motif contributes to the response of the enhancer to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In the context of the dioxin-responsive enhancer, a GC box, representing the DNA binding site for Sp1 (or a related transcription factor), has no detectable intrinsic activity but enhances gene expression when linked to a recognition motif for the liganded Ah receptor, thereby producing a synergistic effect on enhancer function.

Original languageEnglish (US)
Pages (from-to)9676-9681
Number of pages6
JournalJournal of Biological Chemistry
Volume265
Issue number17
StatePublished - Jun 15 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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