Organization and function of a dioxin-responsive enhancer

Joan M. Fisher, Lena Wu, Michael S. Denison, James P. Whitlock

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The dioxin-responsive enhancer upstream of the CYP1A1 gene contains four copies of the recognition motif for the liganded Ah receptor. The results of deletion analyses, linker-scanning analyses, and the analysis of individual enhancer subdomains reveal that each copy of the motif contributes to the response of the enhancer to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In the context of the dioxin-responsive enhancer, a GC box, representing the DNA binding site for Sp1 (or a related transcription factor), has no detectable intrinsic activity but enhances gene expression when linked to a recognition motif for the liganded Ah receptor, thereby producing a synergistic effect on enhancer function.

Original languageEnglish (US)
Pages (from-to)9676-9681
Number of pages6
JournalJournal of Biological Chemistry
Volume265
Issue number17
StatePublished - Jun 15 1990
Externally publishedYes

Fingerprint

Dioxins
Cytochrome P-450 CYP1A1
Gene expression
Transcription Factors
Genes
Binding Sites
Scanning
Gene Expression
DNA
1,4-dioxin
Polychlorinated Dibenzodioxins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Fisher, J. M., Wu, L., Denison, M. S., & Whitlock, J. P. (1990). Organization and function of a dioxin-responsive enhancer. Journal of Biological Chemistry, 265(17), 9676-9681.

Organization and function of a dioxin-responsive enhancer. / Fisher, Joan M.; Wu, Lena; Denison, Michael S.; Whitlock, James P.

In: Journal of Biological Chemistry, Vol. 265, No. 17, 15.06.1990, p. 9676-9681.

Research output: Contribution to journalArticle

Fisher, JM, Wu, L, Denison, MS & Whitlock, JP 1990, 'Organization and function of a dioxin-responsive enhancer', Journal of Biological Chemistry, vol. 265, no. 17, pp. 9676-9681.
Fisher JM, Wu L, Denison MS, Whitlock JP. Organization and function of a dioxin-responsive enhancer. Journal of Biological Chemistry. 1990 Jun 15;265(17):9676-9681.
Fisher, Joan M. ; Wu, Lena ; Denison, Michael S. ; Whitlock, James P. / Organization and function of a dioxin-responsive enhancer. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 17. pp. 9676-9681.
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