Abstract
The dioxin-responsive enhancer upstream of the CYP1A1 gene contains four copies of the recognition motif for the liganded Ah receptor. The results of deletion analyses, linker-scanning analyses, and the analysis of individual enhancer subdomains reveal that each copy of the motif contributes to the response of the enhancer to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In the context of the dioxin-responsive enhancer, a GC box, representing the DNA binding site for Sp1 (or a related transcription factor), has no detectable intrinsic activity but enhances gene expression when linked to a recognition motif for the liganded Ah receptor, thereby producing a synergistic effect on enhancer function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9676-9681 |
| Number of pages | 6 |
| Journal | Journal of Biological Chemistry |
| Volume | 265 |
| Issue number | 17 |
| State | Published - Jun 15 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Organization and function of a dioxin-responsive enhancer. / Fisher, Joan M.; Wu, Lena; Denison, Michael S.; Whitlock, James P.
In: Journal of Biological Chemistry, Vol. 265, No. 17, 15.06.1990, p. 9676-9681.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Organization and function of a dioxin-responsive enhancer
AU - Fisher, Joan M.
AU - Wu, Lena
AU - Denison, Michael S.
AU - Whitlock, James P.
PY - 1990/6/15
Y1 - 1990/6/15
N2 - The dioxin-responsive enhancer upstream of the CYP1A1 gene contains four copies of the recognition motif for the liganded Ah receptor. The results of deletion analyses, linker-scanning analyses, and the analysis of individual enhancer subdomains reveal that each copy of the motif contributes to the response of the enhancer to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In the context of the dioxin-responsive enhancer, a GC box, representing the DNA binding site for Sp1 (or a related transcription factor), has no detectable intrinsic activity but enhances gene expression when linked to a recognition motif for the liganded Ah receptor, thereby producing a synergistic effect on enhancer function.
AB - The dioxin-responsive enhancer upstream of the CYP1A1 gene contains four copies of the recognition motif for the liganded Ah receptor. The results of deletion analyses, linker-scanning analyses, and the analysis of individual enhancer subdomains reveal that each copy of the motif contributes to the response of the enhancer to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In the context of the dioxin-responsive enhancer, a GC box, representing the DNA binding site for Sp1 (or a related transcription factor), has no detectable intrinsic activity but enhances gene expression when linked to a recognition motif for the liganded Ah receptor, thereby producing a synergistic effect on enhancer function.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025324530&partnerID=8YFLogxK
M3 - Article
C2 - 2161840
AN - SCOPUS:0025324530
VL - 265
SP - 9676
EP - 9681
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 17
ER -