Ordered duplex RNA controls capsid architecture in an icosahedral animal virus

Andrew J Fisher, John E. Johnson

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

SMALL spherical viruses are among the simplest replicating systems in biology, yet the factors affecting their assembly, stability and disassembly are still poorly understood. A molecular switch is required for the assembly of icosahedral virus particles containing more than 60 identical subunits because strict symmetry cannot be maintained in subunit packing1. All previously reported viruses with this type of structure use a portion of the capsid protein to regulate interactions between chemically equivalent but structurally distinct interfaces2-4. We have investigated the T = 3 quasiequivalent5 nodaviruses, which are small non-enveloped viruses with a single-stranded RNA genome that infect insects6, mice7 and fish8. They undergo a well-characterized series of steps in assembly and maturation9,10, which in some respects are similar to the picornaviruses11, despite their different capsid architecture. Here we report the X-ray structure of Flock House virus at 3.0 Å resolution, which reveals an ordered RNA duplex of 20 nucleotides and a protein segment that control the subunit interactions in this animal virus. The RNA interacts with a helical protein domain of the subunit that lies inside the capsid shell. One of the helices that binds the RNA is part of a 44-amino-acid polypeptide which is autocatalytically cleaved from the initial subunit translation product after virion assembly. The structure indicates that RNA associated with the cleaved polypeptide may be important in the infection process.

Original languageEnglish (US)
Pages (from-to)176-179
Number of pages4
JournalNature
Volume361
Issue number6408
StatePublished - Jan 14 1993
Externally publishedYes

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Capsid
RNA
Viruses
Virion
Viral Structures
Peptides
Systems Biology
Protein Subunits
Capsid Proteins
Nucleotides
X-Rays
Genome
Amino Acids
Infection
Proteins

ASJC Scopus subject areas

  • General

Cite this

Ordered duplex RNA controls capsid architecture in an icosahedral animal virus. / Fisher, Andrew J; Johnson, John E.

In: Nature, Vol. 361, No. 6408, 14.01.1993, p. 176-179.

Research output: Contribution to journalArticle

Fisher, Andrew J ; Johnson, John E. / Ordered duplex RNA controls capsid architecture in an icosahedral animal virus. In: Nature. 1993 ; Vol. 361, No. 6408. pp. 176-179.
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