Oral administration of trans-resveratrol to guinea pigs increases cardiac DT-diaphorase and catalase activities, and protects isolated atria from menadione toxicity

Maura Floreani, Eleonora Napoli, Luigi Quintieri, Pietro Palatini

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Resveratrol (3,4′,5-trihydroxy-trans-stilbene) is a natural phytoalexin found in grapes and wine. It has antioxidant and antiproliferative activities, and has been shown to induce NAD(P)H:quinone oxidoreductase, also known as DT-diaphorase, in cultured mouse hepatoma cells. DT-diaphorase is a detoxifying enzyme for quinone-containing substances, due to its ability to prevent their one-electron reduction and the consequent generation of reactive oxygen species (ROS). The aim of the present study was to investigate whether oral administration of trans-resveratrol to guinea pigs (60 mg/l in tap water for 16 days, ad libitum) increases cardiac DT-diaphorase and, consequently, reduces the response of isolated atria to 2-methyl-1,4-naphthoquinone (menadione), the positive inotropic effect of which is related to the amount of ROS generated by its cardiac metabolism. In the cardiac tissue of resveratrol-treated animals, DT-diaphorase activity was significantly higher than that measured in control animals, the Vmax of the enzyme reaction being 75.47 ± 3.87 and 50.73 ± 0.63 nmoles/mg protein/min, respectively (p < 0.05). Resveratrol administration also significantly increased the activity of cardiac catalase (32.20 ± 2.39 vs. 25.14 ± 3.85 units/mg protein in treated and control animals, respectively; p < 0.001). As a consequence, menadione metabolism by the cardiac homogenate obtained from resveratrol-treated animals generated a smaller amount of ROS and, in electrically driven left atria, menadione produced a significantly lower increase in the force of contraction than in atria isolated from control animals. These results indicate that oral administration of resveratrol exerts cardioprotection against ROS-mediated menadione toxicity.

Original languageEnglish (US)
Pages (from-to)2741-2750
Number of pages10
JournalLife Sciences
Volume72
Issue number24
DOIs
StatePublished - May 2 2003
Externally publishedYes

Fingerprint

NAD(P)H Dehydrogenase (Quinone)
Vitamin K 3
Catalase
Oral Administration
Toxicity
Guinea Pigs
Animals
Reactive Oxygen Species
Metabolism
Stilbenes
Wine
Vitis
Enzymes
Heart Atria
NAD
resveratrol
Hepatocellular Carcinoma
Oxidoreductases
Proteins
Antioxidants

Keywords

  • Cardiotoxicity
  • Catalase
  • DT-diaphorase
  • Guinea pigs
  • Menadione
  • Resveratrol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Oral administration of trans-resveratrol to guinea pigs increases cardiac DT-diaphorase and catalase activities, and protects isolated atria from menadione toxicity. / Floreani, Maura; Napoli, Eleonora; Quintieri, Luigi; Palatini, Pietro.

In: Life Sciences, Vol. 72, No. 24, 02.05.2003, p. 2741-2750.

Research output: Contribution to journalArticle

@article{b643762e6efc47f8bfea3e23bdcffcd4,
title = "Oral administration of trans-resveratrol to guinea pigs increases cardiac DT-diaphorase and catalase activities, and protects isolated atria from menadione toxicity",
abstract = "Resveratrol (3,4′,5-trihydroxy-trans-stilbene) is a natural phytoalexin found in grapes and wine. It has antioxidant and antiproliferative activities, and has been shown to induce NAD(P)H:quinone oxidoreductase, also known as DT-diaphorase, in cultured mouse hepatoma cells. DT-diaphorase is a detoxifying enzyme for quinone-containing substances, due to its ability to prevent their one-electron reduction and the consequent generation of reactive oxygen species (ROS). The aim of the present study was to investigate whether oral administration of trans-resveratrol to guinea pigs (60 mg/l in tap water for 16 days, ad libitum) increases cardiac DT-diaphorase and, consequently, reduces the response of isolated atria to 2-methyl-1,4-naphthoquinone (menadione), the positive inotropic effect of which is related to the amount of ROS generated by its cardiac metabolism. In the cardiac tissue of resveratrol-treated animals, DT-diaphorase activity was significantly higher than that measured in control animals, the Vmax of the enzyme reaction being 75.47 ± 3.87 and 50.73 ± 0.63 nmoles/mg protein/min, respectively (p < 0.05). Resveratrol administration also significantly increased the activity of cardiac catalase (32.20 ± 2.39 vs. 25.14 ± 3.85 units/mg protein in treated and control animals, respectively; p < 0.001). As a consequence, menadione metabolism by the cardiac homogenate obtained from resveratrol-treated animals generated a smaller amount of ROS and, in electrically driven left atria, menadione produced a significantly lower increase in the force of contraction than in atria isolated from control animals. These results indicate that oral administration of resveratrol exerts cardioprotection against ROS-mediated menadione toxicity.",
keywords = "Cardiotoxicity, Catalase, DT-diaphorase, Guinea pigs, Menadione, Resveratrol",
author = "Maura Floreani and Eleonora Napoli and Luigi Quintieri and Pietro Palatini",
year = "2003",
month = "5",
day = "2",
doi = "10.1016/S0024-3205(03)00179-6",
language = "English (US)",
volume = "72",
pages = "2741--2750",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "24",

}

TY - JOUR

T1 - Oral administration of trans-resveratrol to guinea pigs increases cardiac DT-diaphorase and catalase activities, and protects isolated atria from menadione toxicity

AU - Floreani, Maura

AU - Napoli, Eleonora

AU - Quintieri, Luigi

AU - Palatini, Pietro

PY - 2003/5/2

Y1 - 2003/5/2

N2 - Resveratrol (3,4′,5-trihydroxy-trans-stilbene) is a natural phytoalexin found in grapes and wine. It has antioxidant and antiproliferative activities, and has been shown to induce NAD(P)H:quinone oxidoreductase, also known as DT-diaphorase, in cultured mouse hepatoma cells. DT-diaphorase is a detoxifying enzyme for quinone-containing substances, due to its ability to prevent their one-electron reduction and the consequent generation of reactive oxygen species (ROS). The aim of the present study was to investigate whether oral administration of trans-resveratrol to guinea pigs (60 mg/l in tap water for 16 days, ad libitum) increases cardiac DT-diaphorase and, consequently, reduces the response of isolated atria to 2-methyl-1,4-naphthoquinone (menadione), the positive inotropic effect of which is related to the amount of ROS generated by its cardiac metabolism. In the cardiac tissue of resveratrol-treated animals, DT-diaphorase activity was significantly higher than that measured in control animals, the Vmax of the enzyme reaction being 75.47 ± 3.87 and 50.73 ± 0.63 nmoles/mg protein/min, respectively (p < 0.05). Resveratrol administration also significantly increased the activity of cardiac catalase (32.20 ± 2.39 vs. 25.14 ± 3.85 units/mg protein in treated and control animals, respectively; p < 0.001). As a consequence, menadione metabolism by the cardiac homogenate obtained from resveratrol-treated animals generated a smaller amount of ROS and, in electrically driven left atria, menadione produced a significantly lower increase in the force of contraction than in atria isolated from control animals. These results indicate that oral administration of resveratrol exerts cardioprotection against ROS-mediated menadione toxicity.

AB - Resveratrol (3,4′,5-trihydroxy-trans-stilbene) is a natural phytoalexin found in grapes and wine. It has antioxidant and antiproliferative activities, and has been shown to induce NAD(P)H:quinone oxidoreductase, also known as DT-diaphorase, in cultured mouse hepatoma cells. DT-diaphorase is a detoxifying enzyme for quinone-containing substances, due to its ability to prevent their one-electron reduction and the consequent generation of reactive oxygen species (ROS). The aim of the present study was to investigate whether oral administration of trans-resveratrol to guinea pigs (60 mg/l in tap water for 16 days, ad libitum) increases cardiac DT-diaphorase and, consequently, reduces the response of isolated atria to 2-methyl-1,4-naphthoquinone (menadione), the positive inotropic effect of which is related to the amount of ROS generated by its cardiac metabolism. In the cardiac tissue of resveratrol-treated animals, DT-diaphorase activity was significantly higher than that measured in control animals, the Vmax of the enzyme reaction being 75.47 ± 3.87 and 50.73 ± 0.63 nmoles/mg protein/min, respectively (p < 0.05). Resveratrol administration also significantly increased the activity of cardiac catalase (32.20 ± 2.39 vs. 25.14 ± 3.85 units/mg protein in treated and control animals, respectively; p < 0.001). As a consequence, menadione metabolism by the cardiac homogenate obtained from resveratrol-treated animals generated a smaller amount of ROS and, in electrically driven left atria, menadione produced a significantly lower increase in the force of contraction than in atria isolated from control animals. These results indicate that oral administration of resveratrol exerts cardioprotection against ROS-mediated menadione toxicity.

KW - Cardiotoxicity

KW - Catalase

KW - DT-diaphorase

KW - Guinea pigs

KW - Menadione

KW - Resveratrol

UR - http://www.scopus.com/inward/record.url?scp=0037414403&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037414403&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(03)00179-6

DO - 10.1016/S0024-3205(03)00179-6

M3 - Article

C2 - 12679191

AN - SCOPUS:0037414403

VL - 72

SP - 2741

EP - 2750

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 24

ER -