Oral administration of the nitric oxide biosynthesis inhibitor, N-nitro-l-arginine methyl ester (L-NAME), causes hypertension, but not glucose intolerance or insulin resistance, in rats

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Abstract

While essential hypertension may be characterized by insulin resistance, it is unclear which defect is primary. We therefore compared normotensive Sprague-Dawley male rats who drank N-nitro-l-arginine methyl ester (L-NAME, 1 mg/mL in distilled water), with control rats who drank distilled water. Blood pressure was measured noninvasively, weight was controlled by dietary restriction, and glucose tolerance was assessed via oral glucose tolerance tests (OGTT). Blood pressure rose by the second day of L-NAME treatment, and remained elevated throughout the study, in contrast to the rats drinking water (P < .001). Weight rose similarly in both groups. OGTT were performed after 2 weeks of L-NAME. Serum glucose and insulin responses, assessed by two-way ANOVA, were similar in the two groups (P = NS). In summary, L-NAME administration resulted in hypertension, but not a deterioration in glucose tolerance in diet-controlled Sprague-Dawley rats. We conclude that the insulin resistance of some hypertensive states is not the result of hypertension per se, or increased vasoconstriction, such as might result from inhibition of endogenous nitric oxide synthesis, but rather indicates a fundamental metabolic disorder.

Original languageEnglish (US)
Pages (from-to)1009-1014
Number of pages6
JournalAmerican Journal of Hypertension
Volume8
Issue number10
DOIs
StatePublished - 1995

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Glucose Intolerance
NG-Nitroarginine Methyl Ester
Oral Administration
Insulin Resistance
Nitric Oxide
Hypertension
Glucose Tolerance Test
Glucose
Sprague Dawley Rats
Blood Pressure
Weights and Measures
Water
Vasoconstriction
Drinking Water
Analysis of Variance
Insulin
Diet
arginine methyl ester
Serum
Therapeutics

Keywords

  • hypertension
  • insulin resistance
  • N-nitro-l-arginine methyl ester (L-NAME)
  • Nitric oxide
  • Sprague-Dawley rat

ASJC Scopus subject areas

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Oral administration of the nitric oxide biosynthesis inhibitor, N-nitro-l-arginine methyl ester (L-NAME), causes hypertension, but not glucose intolerance or insulin resistance, in rats",
abstract = "While essential hypertension may be characterized by insulin resistance, it is unclear which defect is primary. We therefore compared normotensive Sprague-Dawley male rats who drank N-nitro-l-arginine methyl ester (L-NAME, 1 mg/mL in distilled water), with control rats who drank distilled water. Blood pressure was measured noninvasively, weight was controlled by dietary restriction, and glucose tolerance was assessed via oral glucose tolerance tests (OGTT). Blood pressure rose by the second day of L-NAME treatment, and remained elevated throughout the study, in contrast to the rats drinking water (P < .001). Weight rose similarly in both groups. OGTT were performed after 2 weeks of L-NAME. Serum glucose and insulin responses, assessed by two-way ANOVA, were similar in the two groups (P = NS). In summary, L-NAME administration resulted in hypertension, but not a deterioration in glucose tolerance in diet-controlled Sprague-Dawley rats. We conclude that the insulin resistance of some hypertensive states is not the result of hypertension per se, or increased vasoconstriction, such as might result from inhibition of endogenous nitric oxide synthesis, but rather indicates a fundamental metabolic disorder.",
keywords = "hypertension, insulin resistance, N-nitro-l-arginine methyl ester (L-NAME), Nitric oxide, Sprague-Dawley rat",
author = "Swislocki, {Arthur L} and Teresa Eason and George Kaysen",
year = "1995",
doi = "10.1016/0895-7061(95)00161-1",
language = "English (US)",
volume = "8",
pages = "1009--1014",
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T1 - Oral administration of the nitric oxide biosynthesis inhibitor, N-nitro-l-arginine methyl ester (L-NAME), causes hypertension, but not glucose intolerance or insulin resistance, in rats

AU - Swislocki, Arthur L

AU - Eason, Teresa

AU - Kaysen, George

PY - 1995

Y1 - 1995

N2 - While essential hypertension may be characterized by insulin resistance, it is unclear which defect is primary. We therefore compared normotensive Sprague-Dawley male rats who drank N-nitro-l-arginine methyl ester (L-NAME, 1 mg/mL in distilled water), with control rats who drank distilled water. Blood pressure was measured noninvasively, weight was controlled by dietary restriction, and glucose tolerance was assessed via oral glucose tolerance tests (OGTT). Blood pressure rose by the second day of L-NAME treatment, and remained elevated throughout the study, in contrast to the rats drinking water (P < .001). Weight rose similarly in both groups. OGTT were performed after 2 weeks of L-NAME. Serum glucose and insulin responses, assessed by two-way ANOVA, were similar in the two groups (P = NS). In summary, L-NAME administration resulted in hypertension, but not a deterioration in glucose tolerance in diet-controlled Sprague-Dawley rats. We conclude that the insulin resistance of some hypertensive states is not the result of hypertension per se, or increased vasoconstriction, such as might result from inhibition of endogenous nitric oxide synthesis, but rather indicates a fundamental metabolic disorder.

AB - While essential hypertension may be characterized by insulin resistance, it is unclear which defect is primary. We therefore compared normotensive Sprague-Dawley male rats who drank N-nitro-l-arginine methyl ester (L-NAME, 1 mg/mL in distilled water), with control rats who drank distilled water. Blood pressure was measured noninvasively, weight was controlled by dietary restriction, and glucose tolerance was assessed via oral glucose tolerance tests (OGTT). Blood pressure rose by the second day of L-NAME treatment, and remained elevated throughout the study, in contrast to the rats drinking water (P < .001). Weight rose similarly in both groups. OGTT were performed after 2 weeks of L-NAME. Serum glucose and insulin responses, assessed by two-way ANOVA, were similar in the two groups (P = NS). In summary, L-NAME administration resulted in hypertension, but not a deterioration in glucose tolerance in diet-controlled Sprague-Dawley rats. We conclude that the insulin resistance of some hypertensive states is not the result of hypertension per se, or increased vasoconstriction, such as might result from inhibition of endogenous nitric oxide synthesis, but rather indicates a fundamental metabolic disorder.

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KW - Nitric oxide

KW - Sprague-Dawley rat

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