Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures

Doris Lam, Heather A. Enright, Sandra K.G. Peters, Monica L. Moya, David A. Soscia, Jose Cadena, Javier A. Alvarado, Kristen S. Kulp, Elizabeth K. Wheeler, Nicholas O. Fischer

Research output: Contribution to journalArticle

Abstract

Background: The emergence of three-dimensional (3D) cell culture in neural tissue engineering has significantly elevated the complexity and relevance of in vitro systems. This is due in large part to the incorporation of biomaterials to impart structural dimensionality on the neuronal cultures. However, a comprehensive understanding of how key seeding parameters affect changes in cell distribution and viability remain unreported. New method: In this study, we systematically evaluated permutations in seeding conditions (i.e., cell concentration and atmospheric CO2 levels) to understand how these affect key parameters in 3D culture characterization (i.e., cell health and distribution). Primary rat cortical neurons (i.e., 2 × 106, 4 × 106, and 1 × 107 cells/mL) were entrapped in collagen blended with ECM proteins (ECM-Collagen) and exposed to atmospheric CO2 (i.e., 0 vs 5% CO2) during fibrillogenesis. Results: At 14 days in vitro (DIV), cell distribution within the hydrogel was dependent on cell concentration and atmospheric CO2 during fibrillogenesis. A uniform distribution of cells was observed in cultures with 2 × 106 and 4 × 106 cells/mL in the presence of 5% CO2, while a heterogeneous distribution was observed in cultures with 1 × 107 cells/mL or in the absence of CO2. Furthermore, increased cell concentration was proportional to the rise in cell death at 14 DIV, although cells remain viable >30 DIV. Comparison with existing methods: ECM-Collagen gels have been shown to increase cell viability of neurons long-term. Conclusion: In using ECM-collagen gels, we highlight the importance of optimizing seeding parameters and thorough 3D culture characterization to understand the neurophysiological responses of these 3D systems.

Original languageEnglish (US)
Article number108460
JournalJournal of Neuroscience Methods
Volume329
DOIs
StatePublished - Jan 1 2020
Externally publishedYes

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Hydrogels
Collagen
Cell Survival
Gels
Neurons
Hydrogel
Biocompatible Materials
Tissue Engineering
Cell Death
Cell Culture Techniques

Keywords

  • 3D Neuronal culture
  • Cortical neurons
  • ECM-collagen hydrogel
  • Entrapment
  • Hydrogel

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures. / Lam, Doris; Enright, Heather A.; Peters, Sandra K.G.; Moya, Monica L.; Soscia, David A.; Cadena, Jose; Alvarado, Javier A.; Kulp, Kristen S.; Wheeler, Elizabeth K.; Fischer, Nicholas O.

In: Journal of Neuroscience Methods, Vol. 329, 108460, 01.01.2020.

Research output: Contribution to journalArticle

Lam, D, Enright, HA, Peters, SKG, Moya, ML, Soscia, DA, Cadena, J, Alvarado, JA, Kulp, KS, Wheeler, EK & Fischer, NO 2020, 'Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures', Journal of Neuroscience Methods, vol. 329, 108460. https://doi.org/10.1016/j.jneumeth.2019.108460
Lam, Doris ; Enright, Heather A. ; Peters, Sandra K.G. ; Moya, Monica L. ; Soscia, David A. ; Cadena, Jose ; Alvarado, Javier A. ; Kulp, Kristen S. ; Wheeler, Elizabeth K. ; Fischer, Nicholas O. / Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures. In: Journal of Neuroscience Methods. 2020 ; Vol. 329.
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abstract = "Background: The emergence of three-dimensional (3D) cell culture in neural tissue engineering has significantly elevated the complexity and relevance of in vitro systems. This is due in large part to the incorporation of biomaterials to impart structural dimensionality on the neuronal cultures. However, a comprehensive understanding of how key seeding parameters affect changes in cell distribution and viability remain unreported. New method: In this study, we systematically evaluated permutations in seeding conditions (i.e., cell concentration and atmospheric CO2 levels) to understand how these affect key parameters in 3D culture characterization (i.e., cell health and distribution). Primary rat cortical neurons (i.e., 2 × 106, 4 × 106, and 1 × 107 cells/mL) were entrapped in collagen blended with ECM proteins (ECM-Collagen) and exposed to atmospheric CO2 (i.e., 0 vs 5{\%} CO2) during fibrillogenesis. Results: At 14 days in vitro (DIV), cell distribution within the hydrogel was dependent on cell concentration and atmospheric CO2 during fibrillogenesis. A uniform distribution of cells was observed in cultures with 2 × 106 and 4 × 106 cells/mL in the presence of 5{\%} CO2, while a heterogeneous distribution was observed in cultures with 1 × 107 cells/mL or in the absence of CO2. Furthermore, increased cell concentration was proportional to the rise in cell death at 14 DIV, although cells remain viable >30 DIV. Comparison with existing methods: ECM-Collagen gels have been shown to increase cell viability of neurons long-term. Conclusion: In using ECM-collagen gels, we highlight the importance of optimizing seeding parameters and thorough 3D culture characterization to understand the neurophysiological responses of these 3D systems.",
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T1 - Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures

AU - Lam, Doris

AU - Enright, Heather A.

AU - Peters, Sandra K.G.

AU - Moya, Monica L.

AU - Soscia, David A.

AU - Cadena, Jose

AU - Alvarado, Javier A.

AU - Kulp, Kristen S.

AU - Wheeler, Elizabeth K.

AU - Fischer, Nicholas O.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Background: The emergence of three-dimensional (3D) cell culture in neural tissue engineering has significantly elevated the complexity and relevance of in vitro systems. This is due in large part to the incorporation of biomaterials to impart structural dimensionality on the neuronal cultures. However, a comprehensive understanding of how key seeding parameters affect changes in cell distribution and viability remain unreported. New method: In this study, we systematically evaluated permutations in seeding conditions (i.e., cell concentration and atmospheric CO2 levels) to understand how these affect key parameters in 3D culture characterization (i.e., cell health and distribution). Primary rat cortical neurons (i.e., 2 × 106, 4 × 106, and 1 × 107 cells/mL) were entrapped in collagen blended with ECM proteins (ECM-Collagen) and exposed to atmospheric CO2 (i.e., 0 vs 5% CO2) during fibrillogenesis. Results: At 14 days in vitro (DIV), cell distribution within the hydrogel was dependent on cell concentration and atmospheric CO2 during fibrillogenesis. A uniform distribution of cells was observed in cultures with 2 × 106 and 4 × 106 cells/mL in the presence of 5% CO2, while a heterogeneous distribution was observed in cultures with 1 × 107 cells/mL or in the absence of CO2. Furthermore, increased cell concentration was proportional to the rise in cell death at 14 DIV, although cells remain viable >30 DIV. Comparison with existing methods: ECM-Collagen gels have been shown to increase cell viability of neurons long-term. Conclusion: In using ECM-collagen gels, we highlight the importance of optimizing seeding parameters and thorough 3D culture characterization to understand the neurophysiological responses of these 3D systems.

AB - Background: The emergence of three-dimensional (3D) cell culture in neural tissue engineering has significantly elevated the complexity and relevance of in vitro systems. This is due in large part to the incorporation of biomaterials to impart structural dimensionality on the neuronal cultures. However, a comprehensive understanding of how key seeding parameters affect changes in cell distribution and viability remain unreported. New method: In this study, we systematically evaluated permutations in seeding conditions (i.e., cell concentration and atmospheric CO2 levels) to understand how these affect key parameters in 3D culture characterization (i.e., cell health and distribution). Primary rat cortical neurons (i.e., 2 × 106, 4 × 106, and 1 × 107 cells/mL) were entrapped in collagen blended with ECM proteins (ECM-Collagen) and exposed to atmospheric CO2 (i.e., 0 vs 5% CO2) during fibrillogenesis. Results: At 14 days in vitro (DIV), cell distribution within the hydrogel was dependent on cell concentration and atmospheric CO2 during fibrillogenesis. A uniform distribution of cells was observed in cultures with 2 × 106 and 4 × 106 cells/mL in the presence of 5% CO2, while a heterogeneous distribution was observed in cultures with 1 × 107 cells/mL or in the absence of CO2. Furthermore, increased cell concentration was proportional to the rise in cell death at 14 DIV, although cells remain viable >30 DIV. Comparison with existing methods: ECM-Collagen gels have been shown to increase cell viability of neurons long-term. Conclusion: In using ECM-collagen gels, we highlight the importance of optimizing seeding parameters and thorough 3D culture characterization to understand the neurophysiological responses of these 3D systems.

KW - 3D Neuronal culture

KW - Cortical neurons

KW - ECM-collagen hydrogel

KW - Entrapment

KW - Hydrogel

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