Optimized methodology for sequential extraction of RNA and protein from small human skin biopsies

Susanne R. Berglund, Chad W. Schwietert, Angela A. Jones, Robin L Stern, Joerg Lehmann, Zelanna Goldberg

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Current translational human studies are moving in the direction of concurrent genomic and proteomic analysis using small clinical samples. Skin tissue, although easily accessible, is difficult to process owing to its natural resistance to mechanical shearing and high levels of RNases and proteases. Currently, these complications result in degraded RNA samples with variable yield. We have developed a method of sequential extraction of high quality RNA and protein from a single 3mm full thickness skin punch biopsy. This method yields 1-2μg of RNA and 150μg of protein, which is usable in many sensitive downstream applications including microarray, quantitative real-time PCR, two-dimensional gel electrophoresis and Western blot analysis.

Original languageEnglish (US)
Pages (from-to)349-353
Number of pages5
JournalJournal of Investigative Dermatology
Volume127
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Biopsy
Skin
RNA
Proteins
Electrophoresis, Gel, Two-Dimensional
Microarrays
Ribonucleases
Electrophoresis
Shearing
Innate Immunity
Proteomics
Real-Time Polymerase Chain Reaction
Peptide Hydrolases
Western Blotting
Gels
Tissue

ASJC Scopus subject areas

  • Dermatology

Cite this

Optimized methodology for sequential extraction of RNA and protein from small human skin biopsies. / Berglund, Susanne R.; Schwietert, Chad W.; Jones, Angela A.; Stern, Robin L; Lehmann, Joerg; Goldberg, Zelanna.

In: Journal of Investigative Dermatology, Vol. 127, No. 2, 02.2007, p. 349-353.

Research output: Contribution to journalArticle

Berglund, Susanne R. ; Schwietert, Chad W. ; Jones, Angela A. ; Stern, Robin L ; Lehmann, Joerg ; Goldberg, Zelanna. / Optimized methodology for sequential extraction of RNA and protein from small human skin biopsies. In: Journal of Investigative Dermatology. 2007 ; Vol. 127, No. 2. pp. 349-353.
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