Optimization of liposome mediated transfection of a neuronal cell line

Andrew McQuillin, Karl D Murray, Christopher J. Etheridge, Luisa Stewart, Robert G. Cooper, Peter M. Brett, Andrew D. Miller, Hugh M D Gurling

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A cell line derived from sensory neurons was transfected with high efficiency using cationic liposomes, formulated from 3β[N-(N',N'- dimethylaminoethane)carbamoyl]cholesterol (DC-Chol) and dioleoyl L-α- phosphatidylethanolamine (DOPE). This is the first time that cationic liposomes of this type have been reported to transfect a neuronal cell line. We used a reporter gene construct expressing β-galactosidase under the control of the cytomegalovirus immediate early promoter and routinely observed transfection efficiencies >40%. Parameters affecting transfection efficiency were examined and the ratio of DNA to liposome proved to be crucial. Liposome formulation procedures and cell transfection protocols devised here will be used as a basis for further in vivo and in vitro work.

Original languageEnglish (US)
Pages (from-to)1481-1484
Number of pages4
JournalNeuroReport
Volume8
Issue number6
StatePublished - 1997
Externally publishedYes

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Keywords

  • DC-Chol/DOPE
  • Liposome
  • Nerve
  • Transfection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

McQuillin, A., Murray, K. D., Etheridge, C. J., Stewart, L., Cooper, R. G., Brett, P. M., Miller, A. D., & Gurling, H. M. D. (1997). Optimization of liposome mediated transfection of a neuronal cell line. NeuroReport, 8(6), 1481-1484.