A cell line derived from sensory neurons was transfected with high efficiency using cationic liposomes, formulated from 3β[N-(N',N'- dimethylaminoethane)carbamoyl]cholesterol (DC-Chol) and dioleoyl L-α- phosphatidylethanolamine (DOPE). This is the first time that cationic liposomes of this type have been reported to transfect a neuronal cell line. We used a reporter gene construct expressing β-galactosidase under the control of the cytomegalovirus immediate early promoter and routinely observed transfection efficiencies >40%. Parameters affecting transfection efficiency were examined and the ratio of DNA to liposome proved to be crucial. Liposome formulation procedures and cell transfection protocols devised here will be used as a basis for further in vivo and in vitro work.
|Original language||English (US)|
|Number of pages||4|
|State||Published - 1997|
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