Optimization of liposome mediated transfection of a neuronal cell line

Andrew McQuillin, Karl D Murray, Christopher J. Etheridge, Luisa Stewart, Robert G. Cooper, Peter M. Brett, Andrew D. Miller, Hugh M D Gurling

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A cell line derived from sensory neurons was transfected with high efficiency using cationic liposomes, formulated from 3β[N-(N',N'- dimethylaminoethane)carbamoyl]cholesterol (DC-Chol) and dioleoyl L-α- phosphatidylethanolamine (DOPE). This is the first time that cationic liposomes of this type have been reported to transfect a neuronal cell line. We used a reporter gene construct expressing β-galactosidase under the control of the cytomegalovirus immediate early promoter and routinely observed transfection efficiencies >40%. Parameters affecting transfection efficiency were examined and the ratio of DNA to liposome proved to be crucial. Liposome formulation procedures and cell transfection protocols devised here will be used as a basis for further in vivo and in vitro work.

Original languageEnglish (US)
Pages (from-to)1481-1484
Number of pages4
JournalNeuroReport
Volume8
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

Liposomes
Transfection
Cell Line
Galactosidases
Sensory Receptor Cells
Cytomegalovirus
Reporter Genes
DNA

Keywords

  • DC-Chol/DOPE
  • Liposome
  • Nerve
  • Transfection

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

McQuillin, A., Murray, K. D., Etheridge, C. J., Stewart, L., Cooper, R. G., Brett, P. M., ... Gurling, H. M. D. (1997). Optimization of liposome mediated transfection of a neuronal cell line. NeuroReport, 8(6), 1481-1484.

Optimization of liposome mediated transfection of a neuronal cell line. / McQuillin, Andrew; Murray, Karl D; Etheridge, Christopher J.; Stewart, Luisa; Cooper, Robert G.; Brett, Peter M.; Miller, Andrew D.; Gurling, Hugh M D.

In: NeuroReport, Vol. 8, No. 6, 1997, p. 1481-1484.

Research output: Contribution to journalArticle

McQuillin, A, Murray, KD, Etheridge, CJ, Stewart, L, Cooper, RG, Brett, PM, Miller, AD & Gurling, HMD 1997, 'Optimization of liposome mediated transfection of a neuronal cell line', NeuroReport, vol. 8, no. 6, pp. 1481-1484.
McQuillin A, Murray KD, Etheridge CJ, Stewart L, Cooper RG, Brett PM et al. Optimization of liposome mediated transfection of a neuronal cell line. NeuroReport. 1997;8(6):1481-1484.
McQuillin, Andrew ; Murray, Karl D ; Etheridge, Christopher J. ; Stewart, Luisa ; Cooper, Robert G. ; Brett, Peter M. ; Miller, Andrew D. ; Gurling, Hugh M D. / Optimization of liposome mediated transfection of a neuronal cell line. In: NeuroReport. 1997 ; Vol. 8, No. 6. pp. 1481-1484.
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